Volume 78, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


To date, no immune correlates for blood stage–specific immunity against malaria parasites have been identified. Growth and/or invasion inhibition assays using sera from Phase 2a/b trials will aid in determining whether correlations with protective immunity can be established for these assays. A major constraint in the ability to evaluate functional antibody activities from populations in endemic areas is the relatively limited availability of sufficient sample quantity. For this reason, we developed a miniaturized and high-throughput method to measure growth inhibitory activity by quantification of parasite lactate dehydrogenase (pLDH) in a 384-microtiter plate format. This culture method can be extended beyond the pLDH-based readout to other techniques commonly used to determine growth/invasion inhibition.


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  • Received : 01 Nov 2007
  • Accepted : 15 Dec 2007

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