Volume 78, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Both malaria treatment and prophylaxis target the parasite dihydropteroate synthase (DHPS) and dihydrofolate reductase (DHFR) enzymes. Specific point mutations in these genes confer resistance to sulfadoxine-pyrimethamine (SP) in both and . We used direct sequencing to examine the prevalence of point mutations in and in 160 isolates collected from areas along the international borders of Thailand. Results show that the majority of the isolates harbored a quadruple mutant allele of and a double mutant allele of , but the distribution was not uniform. The highly mutant allele combination was especially prevalent along the Thai-Myanmar border, whereas the majority of the isolates from areas along the Thai-Cambodian and Thai-Malaysian borders carried double mutant alleles of and single mutant alleles of Novel mutations that have not been identified previously at codon 512 of (K512M, K512E, K512T) were also found.


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  • Received : 27 Aug 2007
  • Accepted : 21 Nov 2007

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