Volume 78, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


American cutaneous leishmaniasis (ACL) has been characterized as a zoonotic disease. However, peridomestic and domestic transmission have been recorded in at least nine countries in Central and South America. The present study was undertaken to identify the etiologic agent of a peridomestic epidemic of ACL in the Department of Tolima, Colombia. isolates were obtained during the diagnosis of 56 patients with ACL who consulted the local leishmaniasis control program in three municipalities in Tolima. Species were identified using monoclonal antibodies and isoenzyme electrophoresis. A total of 53 (94.6%) of 56 isolates were identified as () . Three isolates (5.4%) were identified as () () is the probable etiologic agent of the largest epidemic of cutaneous leishmaniasis recorded in Colombia. This species has not previously been reported outside the Amazon and southeastern regions of Colombia, and has not been described in the peridomestic setting or linked with an epidemic.


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  1. Salomon O, Sosa Estani S, Gomez A, Segura E, 1992. Sandflies associated with a tegumentary leishmaniasis focus in Salta, Argentina. Mem Inst Oswaldo Cruz 87 : S223. [Google Scholar]
  2. Le Pont F, Mouchet J, Desjeux P, 1989. Leishmaniasis in Bolivia–VI. Observations on Lutzomyia nuneztovari anglesi Le Pont & Desjeux, 1984 the presumed vector of tegumentary leishmaniasis in the Yungas focus. Mem Inst Oswaldo Cruz 84 : 277–278. [Google Scholar]
  3. Rangel EF, de Souza NA, Wermelinger ED, Barbosa AF, 1984. Natural infection of Lutzomyia intermedia Lutz & Neiva, 1912, in an endemic area of visceral leishmaniasis of Rio de Janeiro. Mem Inst Oswaldo Cruz 79 : 395–396. [Google Scholar]
  4. de Queiroz RG, Vasconcelos Ide A, Vasconcelos AW, Pessoa FA, de Sousa RN, David JR, 1994. Cutaneous leishmaniasis in Ceara state in northeastern Brazil: incrimination of Lutzomyia whitmani (Diptera: Psychodidae) as a vector of Leishmania braziliensis in baturite municipality. Am J Trop Med Hyg 50 : 693–698. [Google Scholar]
  5. Zeledon R, Murillo J, Gutierrez H, 1985. Flebótomos antropófilos y leishmaniasis cutánea en Costa Rica. Bol Oficina Sanit Panam 99 : 163–171. [Google Scholar]
  6. Herrero M, Rojas J, Jimenez A, Zeledon R, Pereira R, Gutierrez H, 1992. Phlebotomine sandflies associated with human houses in an endemic area for cutaneous leishmaniasis in Costa Rica. Wijeyaratne P, Goodman T, Espinal C, eds. Leishmaniasis Control Strategies: A Critical Evaluation of International Development Research Centre (IDRC) Supported Research. Proceedings of an International Workshop. Ottawa, Ontario, Canada: International Development Research Centre Press, 229–241.
  7. Mouchet J, Le Pont F, Leon R, Echeverria R, Guderian RH, 1994. Leishmaniasis in Ecuador. 5. Leishmaniasis and anthropization on the Pacific coast. Ann Soc Belg Med Trop 74 : 35–41. [Google Scholar]
  8. Christensen HA, Fairchild GB, Herrer A, Johnson CM, Young DG, de Vasquez AM, 1983. The ecology of cutaneous leishmaniasis in the Republic of Panama. J Med Entomol 20 : 463–484. [Google Scholar]
  9. Lainson R, 1989. Demographic changes and their influence on the epidemiology of the American leishmaniasis. Service M, ed. Demography of Vector-Borne Disease. Boca Raton, FL: CRC Press, 85–106.
  10. Reithinger R, Davies CR, 1999. Is the domestic dog (Canis familiaris) a reservoir host of American cutaneous leishmaniasis? A critical review of the current evidence. Am J Trop Med Hyg 61 : 530–541. [Google Scholar]
  11. Montoya J, Jaramillo C, Palma G, Gomez T, Segura I, Travi B, 1990. Report of an epidemic outbreak of tegumentary leishmaniasis in a coffee-growing area of Colombia. Mem Inst Oswaldo Cruz 85 : 119–121. [Google Scholar]
  12. Desjeux P, 2002. Urbanization: an increasing risk factor for leishmaniasis. Wkly Epidemiol Rec 77 : 365–370. [Google Scholar]
  13. Gutierrez de Oliveira A, Bianchi Galati E, de Oliveira O, de Oliveira G, Cabello Espindola I, Cavalheiros Dorval M, Peç anha R, 2006. Abundance of Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae) and urban transmission of visceral leishmaniasis in Campo Grande, state of Mato Grosso do Sul, Brazil. Mem Inst Oswaldo Cruz 101 : 869–874. [Google Scholar]
  14. Martinez E, Le Pont F, Torrez M, Telleria J, Vargas F, Munoz M, de Doncker S, Dujardin JC, Dujardin JP, 1998. A new focus of cutaneous leishmaniasis due to Leishmania amazonensis in a Sub Andean region of Bolivia. Acta Trop 71 : 97–106. [Google Scholar]
  15. Martinez E, Le Pont F, Torrez M, Telleria J, Vargas F, Dujardin JC, Dujardin JP, 1999. Lutzomyia nuneztovari anglesi (Le Pont & Desjeux, 1984) as a vector of Leishmania amazonensis in a sub-Andean leishmaniasis focus of Bolivia. Am J Trop Med Hyg 61 : 846–849. [Google Scholar]
  16. Bonfante-Garrido R, Morillo N, Torres R, 1981. Leishmaniasis cutánea canina en Venezuela. Bol Of Sanit Panam 91 : 161–165. [Google Scholar]
  17. Falqueto A, Coura J, Barros G, Grimaldi G, Sessa P, Jesús A, 1986. Participação do cão no ciclo de transmissão da leishmaniose tegumentar no município de Viana, Estado o Espírito Santo, Brasil. Mem Inst Oswaldo Cruz 81 : 155–163. [Google Scholar]
  18. Falqueto A, Sessa P, Varejao J, Barros G, Momen H, Grimaldi JG, 1991. Leishmaniasis due to Leishmania braziliensis in Espírito Santo State, Brazil. Further evidence on the role of dogs as a reservoir of infection for humans. Mem Inst Oswaldo Cruz 86 : 499–500. [Google Scholar]
  19. Aguilar CM, Rangel EF, Garcia L, Fernandez E, Momen H, Grimaldi Filho G, De Vargas Z, 1989. Zoonotic cutaneous leishmaniasis due to Leishmania (Viannia) braziliensis associated with domestic animals in Venezuela and Brazil. Mem Inst Oswaldo Cruz 84 : 19–28. [Google Scholar]
  20. Desjeux P, 1991. Information on the Epidemiology and Control of the Leishmaniases by Country and Territory. Geneva: World Health Organization. WHO/LEISH/91.30: 35–47.
  21. Reithinger R, Espinoza JC, Courtenay O, Davies CR, 2003. Evaluation of PCR as a diagnostic mass-screening tool to detect Leishmania (Viannia) spp. in domestic dogs (Canis familiaris). J Clin Microbiol 41 : 1486–1493. [Google Scholar]
  22. Reithinger R, Espinoza JC, Davies CR, 2003. The transmission dynamics of canine American cutaneous leishmaniasis in Huanuco, Peru. Am J Trop Med Hyg 69 : 473–480. [Google Scholar]
  23. Padilla AM, Marco JD, Diosque P, Segura MA, Mora MC, Fernandez MM, Malchiodi EL, Basombrio MA, 2002. Canine infection and the possible role of dogs in the transmission of American tegumentary leishmaniosis in Salta, Argentina. Vet Parasitol 110 : 1–10. [Google Scholar]
  24. Hernández D, Rojas E, Scorza J, Jorquera A, 2006. Infectividad del perro (Canis familiaris) para Lutzomyia youngi en Trujillo, Venezuela. Biomedica (Bogota) 26 : 242–248. [Google Scholar]
  25. Travi B, 2006. Leishmania (Viannia) braziliensis infection in two Colombian dogs: a note on infectivity for sand flies and response to treatment. Biomedica (Bogota) 26 : 249–253. [Google Scholar]
  26. Corredor A, Kreutzer RD, Tesh RB, Boshell J, Palau MT, Caceres E, Duque S, Pelaez D, Rodriguez G, Nichols S, 1990. Distribution and etiology of leishmaniasis in Colombia. Am J Trop Med Hyg 42 : 206–214. [Google Scholar]
  27. Saravia NG, Weigle K, Navas C, Segura I, Valderrama L, Valencia AZ, Escorcia B, McMahon-Pratt D, 2002. Heterogeneity, geographic distribution, and pathogenicity of serodemes of Leishmania viannia in Colombia. Am J Trop Med Hyg 66 : 738–744. [Google Scholar]
  28. Ovalle CE, Porras L, Rey M, Rios M, Camargo YC, 2006. Geographic distribution of Leishmania species isolated from patients at the National Institute of Dermatology Federico Lleras Acosta E.S.E., 1995–2005. Biomedica (Bogota) 26 (Suppl 1): 145–151. [Google Scholar]
  29. Rodríguez G, Corredor A, Cáceres E, Cassiano G, Arroyo C, Palau M, 1985. Leishmaniasis difusa. Biomedica (Bogota) 5 : 95–111. [Google Scholar]
  30. Sierra D, Ochoa M, Calle JI, Garcia G, Colorado D, Velez ID, 2006. Leishmania (Leishmania) mexicana in the village of San Matias, municipality of Gomez Plata, North West of Antioquia, Colombia. Biomedica (Bogota) 26 (Suppl 1): 232–235. [Google Scholar]
  31. Morales A, Corredor A, Caceres E, Ibagos A, Rodriguez C, 1981. Aislamiento de tres cepas de Leishmania a partir de Lu. trapidoi en Colombia. Biomedica (Bogota) 1 : 198–207. [Google Scholar]
  32. Alexander B, Usma MC, Cadena H, Quesada BL, Solarte Y, Roa W, Montoya J, Jaramillo C, Travi BL, 1995. Phlebotomine sandflies associated with a focus of cutaneous leishmaniasis in Valle del Cauca, Colombia. Med Vet Entomol 9 : 273–278. [Google Scholar]
  33. Munoz G, Davies CR, 2006. Leishmania panamensis transmission in the domestic environment: the results of a prospective epidemiological survey in Santander, Colombia. Biomedica (Bogota) 26 (Suppl 1): 131–144. [Google Scholar]
  34. Santamaria E, Ponce N, Zipa Y, Ferro C, 2006. Presence of infected vectors of Leishmania (V.) panamensis within dwellings in two endemic foci in the foothill of the middle Magdalena valley, western Boyaca, Colombia. Biomedica (Bogota) 26 (Suppl 1): 82–94. [Google Scholar]
  35. Lainson R, Shaw JJ, Silveira FT, de Souza AA, Braga RR, Ishikawa EA, 1994. The dermal leishmaniases of Brazil, with special reference to the eco-epidemiology of the disease in Amazonia. Mem Inst Oswaldo Cruz 89 : 435–443. [Google Scholar]
  36. Ready P, Lainson R, Shaw J, Ward R, 1986. The ecology of Lutzomyia umbratilis (Diptera, Psychodidae), the major vector to man of Leishmania braziliensis guyanensis in northeastern Amazonian Brazil. Bull Ent Res 76 : 21–40. [Google Scholar]
  37. Pardo RH, Cabrera OL, Becerra J, Fuya P, Ferro C, 2006. Lutzomyia longiflocosa as suspected vector of cutaneous leishmaniasis in a focus of cutaneous leishmaniasis on the sub-andean region of Tolima department, Colombia, and the knowledge on sandflies by the inhabitants. Biomedica (Bogota) 26 (Suppl 1): 95–108. [Google Scholar]
  38. Saravia NG, Holguin AF, McMahon-Pratt D, D’Alessandro A, 1985. Mucocutaneous leishmaniasis in Colombia: Leishmania braziliensis subspecies diversity. Am J Trop Med Hyg 34 : 714–720. [Google Scholar]
  39. McMahon-Pratt D, David J, 1981. Monoclonal antibodies that distinguish between New World species of Leishmania. Nature 291 : 581–593. [Google Scholar]
  40. McMahon-Pratt D, Bennett E, David JR, 1982. Monoclonal antibodies that distinguish subspecies of Leishmania braziliensis. J Immunol 129 : 926–927. [Google Scholar]
  41. Organización Mundial de la Salud, 1993. Uso de anticuerpos monoclonales en la identificación de Leishmania de importancia médica en Latinoamérica. Manual de Procedimientos, Programa Especial para Investigación y Entrenamiento en Enfermedades Tropicales. Ginebra, Colombia: Organización Mundial de la Salud, 1–20.
  42. Lanham SM, Grendon JM, Miles MA, Povoa MM, Almeida de Souza AA, 1981. A comparison of electrophoretic methods for isoenzyme characterization of trypanosomatids. I: Standard stocks of Trypanosoma cruzi zymodemes from northeast Brazil. Trans R Soc Trop Med Hyg 75 : 742–750. [Google Scholar]
  43. Cupolillo E, Grimaldi G Jr, Momen H, 1994. A general classification of New World Leishmania using numerical zymotaxonomy. Am J Trop Med Hyg 50 : 296–311. [Google Scholar]
  44. Saravia NG, Segura I, Holguin AF, Santrich C, Valderrama L, Ocampo C, 1998. Epidemiologic, genetic, and clinical associations among phenotypically distinct populations of Leishmania (Viannia ) in Colombia. Am J Trop Med Hyg 59 : 86–94. [Google Scholar]
  45. Romero GA, Guerra MV, Paes MG, Macêdo VO, 2001. Comparison of cutaneous leishmaniasis due to Leishmania (Viannia) braziliensis and L. (V.) guyanensis in Brazil: therapeutic response to meglumine antimoniate. Am J Trop Med Hyg 65 : 456–465. [Google Scholar]
  46. Garcia AL, Tellez T, Parrado R, Rojas E, Bermudez H, Dujardin JC, 2007. Epidemiological monitoring of American tegumentary leishmaniasis: molecular characterization of a peridomestic transmission cycle in the Amazonian lowlands of Bolivia. Trans R Soc Trop Med Hyg 101 : 1208–1213. [Google Scholar]
  47. Marco JD, Barroso PA, Calvopina M, Kumazawa H, Furuya M, Korenaga M, Cajal SP, Mora MC, Rea MM, Borda CE, Basombrio MA, Taranto NJ, Hashiguchi Y, 2005. Species assignation of Leishmania from human and canine American tegumentary leishmaniasis cases by multilocus enzyme electrophoresis in North Argentina. Am J Trop Med Hyg 72 : 606–611. [Google Scholar]
  48. Balbino VQ, Marcondes CB, Alexander B, Luna LK, Lucena MM, Mendes AC, Andrade PP, 2001. First report of Lutzomyia (Nyssomyia) umbratilis Ward & Frahia, 1977 outside of Amazonian Region, in Recife, State of Pernambuco, Brazil (Diptera: Psychodidae: Phlebotominae). Mem Inst Oswaldo Cruz 96 : 315–317. [Google Scholar]
  49. Young D, Duncan M, 1994. Guide to the identification and geographic distribution of Lutzomyia sand flies in Mexico, the West Indies, Central and South America (Diptera: Psychodidae). Mem Entomol Inst 54 : 1–881. [Google Scholar]
  50. Cardenas R, Pabón E, Anaya H, Sandoval C, 2005. Presencia de Lutzomyia longiflocosa (Diptera: Psychodidae) en el foco de leishmaniasis tegumentaria americana del municipio de Abrego, Norte de Santander. Primer registro para el departamento. Rev Inst Facultad Salud Universidad de Pamplona 3 : 7–14. [Google Scholar]
  51. Cardenas R, Romo G, Santamaria E, Ferro C, 1999. Lutzomyia longiflocosa (Diptera:Psychodidae) posible vector en el foco de leishmaniasis cutánea del municipio de Planadas, zona cafetera del Tolima. Biomedica (Bogota) 19 : 239–244. [Google Scholar]
  52. Santamaria E, Castillo M, Cardenas E, Bello F, Ayala M, Ferro C, 1998. Transmisión experimental de Leishmania braziliensis a hamster por picadura de Lutzomyia longiflocosa (Dipetera: Psychodidae) provenientes de un foco endémico en la zona cafetera colombiana. Médicas UIS 12 : 279–284. [Google Scholar]
  53. Arevalo J, Ramirez L, Adaui V, Zimic M, Tulliano G, Miranda-Verastegui C, Lazo M, Loayza-Muro R, de Doncker S, Maurer A, Chappuis F, Dujardin JC, Llanos-Cuentas A, 2007. Influence of Leishmania (Viannia) species on the response to antimonial treatment in patients with American tegumentary leishmaniasis. J Infect Dis 195 : 1846–1851. [Google Scholar]
  54. Soto J, Arana BA, Toledo J, Rizzo N, Vega JC, Diaz A, Luz M, Gutierrez P, Arboleda M, Berman JD, Junge K, Engel J, Sindermann H, 2004. Miltefosine for new world cutaneous leishmaniasis. Clin Infect Dis 38 : 1266–1272. [Google Scholar]
  55. Navin TR, Arana BA, Arana FE, Berman JD, Chajón JF, 1992. Placebo-controlled clinical trial of sodium stibogluconate (Pentostam) versus ketoconazole for treating cutaneous leishmaniasis in Guatemala. J Infect Dis 165 : 528–534. [Google Scholar]
  56. Tojal da Silva AC, Cupolillo E, Volpini AC, Almeida R, Sierra Romero GA, 2006. Species diversity causing human cutaneous leishmaniasis in Rio Branco, state of Acre, Brazil. Trop Med Int Health 11 : 1388–1398. [Google Scholar]
  57. Lucas CM, Franke ED, Cachay MI, Tejada A, Cruz ME, Kreutzer RD, Barker DC, McCann SH, Watts DM, 1998. Geographic distribution and clinical description of leishmaniasis cases in Peru. Am J Trop Med Hyg 59 : 312–317. [Google Scholar]
  58. Calvopina M, Armijos RX, Hashiguchi Y, 2004. Epidemiology of leishmaniasis in Ecuador: current status of knowledge–a review. Mem Inst Oswaldo Cruz 99 : 663–672. [Google Scholar]
  59. Calvopina M, Armijos RX, Marco JD, Uezato H, Kato H, Gomez EA, Korenaga M, Barroso PA, Mimori T, Cooper PJ, Nonaka S, Hashiguchi Y, 2006. Leishmania isoenzyme polymorphisms in Ecuador: relationships with geographic distribution and clinical presentation. BMC Infect Dis 6 : 139. [Google Scholar]
  60. Rotureau B, 2006. Ecology of the Leishmania species in the Guianan ecoregion complex. Am J Trop Med Hyg 74 : 81–96. [Google Scholar]
  61. Rotureau B, Ravel C, Nacher M, Couppie P, Curtet I, Dedet JP, Carme B, 2006. Molecular epidemiology of Leishmania (Viannia) guyanensis in French Guiana. J Clin Microbiol 44 : 468–473. [Google Scholar]
  62. Bonfante-Garrido R, Melendez E, Barroeta S, de Alejos MA, Momen H, Cupolillo E, McMahon-Pratt D, Grimaldi G Jr, 1992. Cutaneous leishmaniasis in western Venezuela caused by infection with Leishmania venezuelensis and L. braziliensis variants. Trans R Soc Trop Med Hyg 86 : 141–148. [Google Scholar]
  63. Desjeux P, Dedet JP, 1989. Isoenzyme characterization of 112 Leishmania isolates from French Guiana. Trans R Soc Trop Med Hyg 83 : 610–612. [Google Scholar]
  64. Grimaldi G Jr, David JR, McMahon-Pratt D, 1987. Identification and distribution of New World Leishmania species characterized by serodeme analysis using monoclonal antibodies. Am J Trop Med Hyg 36 : 270–287. [Google Scholar]

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  • Received : 31 Jul 2007
  • Accepted : 21 Oct 2007

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