Volume 78, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


With the objective of developing a simpler diagnostic alternative, a rapid immunochromatographic test (BoiICT) was constructed for the simultaneous detection of - and -specific antibodies using recombinant merozoite surface antigen-2c and recombinant rhoptry-associated protein-1. The BoiICT selectively detected specific antibodies to and . All sera from cattle infected with other protozoan parasites (i.e., and ) showed negative results in the BoiICT. The relative sensitivity and specificity for detecting antibody to were 96.7% (29 of 30) and 91.3% (73 of 80), respectively. The relative sensitivity and specificity for detecting antibody to were 96.7% (29 of 30) and 92.5% (74 of 80), respectively. These findings indicate that the BoiICT is useful for fast field diagnostic assessment of bovine babesioses without any laboratory equipment.


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  1. McCosker PJ, 1981. The global importance of babesiosis. Ristic M, Kreier JP, eds. Babesiosis. New York: Academic Press, 1–24.
  2. Bock R, Jackson L, de Vos A, Jorgensen W, 2004. Babesiosis of cattle. Parasitology 129 (Suppl): S247–S269. [Google Scholar]
  3. Boonchit S, Alhassan A, Chan B, Xuan X, Yokoyama N, Ooshiro M, Goff WL, Waghela SD, Wagner G, Igarashi I, 2006. Expression of C-terminal truncated and full-length Babesia bigemina rhoptry-associated protein 1 and their potential use in enzyme-linked immunosorbent assay. Vet Parasitol 137 : 28–35. [Google Scholar]
  4. Boonchit S, Xuan X, Yokoyama N, Goff WL, Wagner G, Igarashi I, 2002. Evaluation of an enzyme-linked immunosorbent assay with recombinant rhoptry-associated protein 1 antigen against Babesia bovis for the detection of specific antibodies in cattle. J Clin Microbiol 40 : 3771–3775. [Google Scholar]
  5. Boonchit S, Xuan X, Yokoyama N, Goff WL, Waghela SD, Wagner D, Igarashi I, 2004. Improved enzyme-linked immunosorbent assay using C-terminal truncated recombinant antigens of Babesia bovis rhoptry-associated protein-1 for detection of specific antibodies. J Clin Microbiol 42 : 1601–1604. [Google Scholar]
  6. Bose R, Jorgensen WK, Dalgliesh RJ, Friedhoff KT, de Vos AJ, 1995. Current state and future trends in the diagnosis of babesiosis. Vet Parasitol 57 : 61–74. [Google Scholar]
  7. Ferrer D, Castella J, Gutierez JF, Lavin S, Marco I, 1998. Seroprevalence of Babesia ovis in mouflon sheep in Spain. J Wildl Dis 34 : 637–639. [Google Scholar]
  8. Kim CM, Alhassan A, Verdida RA, Yokoyama N, Xuan X, Fujisaki K, Kawazu S, Igarashi I, 2007. Development of two immunochromatographic tests for the serodiagnosis of bovine babesiosis. Vet Parasitol 148 : 137–143. [Google Scholar]
  9. Molloy JB, Bowles PM, Bock RE, Turton JA, Katsande TC, Katende JM, Mabikacheche LG, Waldron SJ, Blight JW, Dalgliesh RJ, 1998. Evaluation of an ELISA for detection of antibodies to Babesia bovis in cattle in Australia and Zimbabwe. Prev Vet Med 33 : 59–67. [Google Scholar]
  10. Reiter I, Weiland G, 1989. Recently developed methods for the detection of babesial infections. Trans R Soc Trop Med Hyg 83 : 21–23. [Google Scholar]
  11. Wright IG, 1990. Immunodiagnosis of and immunoprophylaxis against the hemoparasites Babesia sp. and Anaplasma sp. in domestic animals. Rev Sci Tech Off Int Epizoot 9 : 345–356. [Google Scholar]
  12. de Saeger S, van Peteghem C, 1996. Dipstick enzyme immunoassay to detect Fusarium T-2 toxin in wheat. Appl Environ Microbiol 62 : 1880–1884. [Google Scholar]
  13. Shiff CJ, Premji Z, Minjas JN, 1993. The rapid manual Para-Sight-F test. A new diagnostic tool for Plasmodium falciparum infection. Trans R Soc Trop Med Hyg 87 : 646–648. [Google Scholar]
  14. Sumathy S, Thyagarajan SP, Latif R, Madanagopalan N, Raguram K, Rajasambandam P, Gowans E, 1992. A dipstick immunobinding enzyme-linked immunosorbent assay for serodiagnosis of hepatitis B and delta virus infections. J Virol Methods 38 : 145–152. [Google Scholar]
  15. Huang X, Xuan X, Verdida RA, Zhang S, Yokoyama N, Xu L, Igarashi I, 2006. Immunochromatographic test for simultaneous serodiagnosis of Babesia caballi and B. equi infections in horses. Clin Vaccine Immunol 13 : 553–555. [Google Scholar]
  16. Huang X, Xuan X, Hirata H, Yokoyama N, Xu L, Suzuki N, Igarashi I, 2004. Rapid immunochromatographic test using recombinant SAG2 for detection of antibodies against Toxoplasma gondii in cats. J Clin Microbiol 42 : 351–353. [Google Scholar]
  17. Mills CD, Burgess DCH, Taylor HJ, Kain KC, 1999. Evaluation of a rapid and inexpensive dipstick assay for the diagnosis of Plasmodium falciparum malaria. Bull World Health Organ 77 : 553–558. [Google Scholar]
  18. Wright IG, Goodger BV, 1988. Pathogenesis of babesiosis. Ristic M, ed. Babesiosis of Domestic Animals and Man. Boca Raton, FL: CRC Press, 99–118.
  19. Barros SL, Madruga CR, Araujo FR, Menk CF, de Almeida MA, Melo EP, Kessler RH, 2005. Serological survey of Babesia bovis, Babesia bigemina, and Anaplasma marginale antibodies in cattle from the semi-arid region of the state of Bahia, Brazil, by enzyme-linked immunosorbent assays. Mem Inst Oswaldo Cruz 100 : 513–517. [Google Scholar]
  20. Wilkowsky SE, Farber M, Echaide I, Torioni de Echaide S, Zamorano PI, Dominguez M, Suarez CE, Florin-Christensen M, 2003. Babesia bovis merozoite surface protein-2c (MSA-2c) contains highly immunogenic, conserved B-cell epitopes that elicit neutralization-sensitive antibodies in cattle. Mol Biochem Parasitol 127 : 133–141. [Google Scholar]
  21. Suarez CE, Florin-Christensen M, Hines SA, Palmer GH, Brown WC, McElwain TF, 2000. Characterization of allelic variation in the Babesia bovis merozoite surface antigen 1 (MSA-1) locus and identification of a cross-reactive inhibition-sensitive MSA-1 epitope. Infect Immun 68 : 6865–6870. [Google Scholar]
  22. Hotzel I, Suarez CE, McElwain TF, Palmer GH, 1997. Genetic variation in the dimorphic regions of RAP-1 genes and rap-1 loci of Babesia bigemina. Mol Biochem Parasitol 2 : 479–489. [Google Scholar]
  23. Levi MG, Ristic M, 1980. Babesia bovis: continuous cultivation in a microaerophilus stationary phase culture. Science 207 : 1218–1220. [Google Scholar]
  24. Chahan B, Gaturaga I, Huang X, Liao M, Fukumoto S, Hirata H, Nishikawa Y, Suzuki H, Sugimoto C, Nagasawa H, Fujisaki K, Igarashi I, Mikami T, Xuan X, 2003. Serodiagnosis of Neospora caninum infection in cattle by enzyme-linked immunosorbent assay with recombinant truncated NcSAG1. Vet Parasitol 118 : 177–185. [Google Scholar]
  25. Takashima Y, Xuan X, Kimata I, Iseki M, Kodama Y, Nagane N, Nagasawa H, Matsumoto Y, Mikami T, Otsuka H, 2003. Recombinant bovine herpesvirus-1 expressing p23 protein of Cryptosporidium parvum induces neutralizing antibodies in rabbits. J Parasitol 89 : 276–282. [Google Scholar]
  26. Pottumarthy S, Morris AJ, Harrison AC, Wells VC, 1999. Evaluation of the tuberculin gamma interferon assay; potential to replace the Mantoux skin test. J Clin Microbiol 37 : 3229–3232. [Google Scholar]
  27. Chitnis CE, Blackman MJ, 2000. Host cell invasion by malaria parasites. Parasitol Today 16 : 411–415. [Google Scholar]
  28. Cowman AF, Baldi DL, Duraisingh M, Healer J, Mills KE, O’Donnell RA, Thompson J, Triglia T, Wickham ME, Crabb BS, 2002. Functional analysis of Plasmodium falciparum merozoite antigens: implications for erythrocyte invasion and vaccine development. Philos Trans R Soc Lond B Biol Sci 357 : 25–33. [Google Scholar]
  29. Egan AF, Burghaus P, Druilhe P, Holder AA, Riley EM, 1999. Human antibodies to the 19kDa C-terminal fragment of Plasmodium falciparum merozoite surface protein 1 inhibit parasite growth in vitro. Parasite Immunol 21 : 133–139. [Google Scholar]
  30. Cowman AF, Bernard O, Stewart N, Kemp D, 1984. Genes of the protozoan parasite Babesia bovis that rearrange to produce RNA species with different sequences. Cell 37 : 653–660. [Google Scholar]
  31. Chan R, Chen J, York MK, Setijono N, Kaplan RL, Graham F, Tanowitz HB, 2000. Evaluation of a combination rapid immunoassay for detection of Giardia and Cryptosporidium antigens. J Clin Microbiol 38 : 393–394. [Google Scholar]
  32. Mohebali M, Taran M, Zarei Z, 2004. Rapid detection of Leishmania infantum infection in dogs: comparative study using an immunochromatographic dipstick rk39 test and direct agglutination. Vet Parasitol 121 : 239–245. [Google Scholar]
  33. Reithinger R, Quinnell RJ, Alexander B, Davies CR, 2002. Rapid detection of Leishmania infantum infection in dogs: comparative study using an immunochromatographic dipstick test, enzyme-linked immunosorbent assay, and PCR. J Clin Microbiol 40 : 2352–2356. [Google Scholar]

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  • Received : 14 Jul 2007
  • Accepted : 04 Oct 2007

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