1921
Volume 77, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

A recently described loop-mediated isothermal polymerase chain reaction (LAMP) for molecular detection of was compared with microscopy, PfHRP2-based rapid diagnostic test (RDT), and nested polymerase chain reaction (PCR) as the “gold standard” in 115 Bangladeshi in-patients with fever. DNA extraction for LAMP was conducted by conventional methods or simple heating of the sample; test results were either assessed visually or by gel electrophoresis. Conventional DNA extraction followed by gel electrophoresis had the highest agreement with the reference method (81.7%, κ = 0.64), with a sensitivity (95% CI) of 76.1% (68.3–83.9%), comparable to RDT and microscopy, but a specificity of 89.6% (84.0–95.2%) compared with 100% for RDT and microscopy. DNA extraction by heat treatment deteriorated specificity to unacceptable levels. LAMP enables molecular diagnosis of falciparum malaria in settings with limited technical resources but will need further optimization. The results are in contrast with a higher accuracy reported in an earlier study comparing LAMP with a non-validated PCR method.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.2007.77.972
2007-11-01
2017-09-22
Loading full text...

Full text loading...

/deliver/fulltext/14761645/77/5/0770972.html?itemId=/content/journals/10.4269/ajtmh.2007.77.972&mimeType=html&fmt=ahah

References

  1. Reyburn H, Mbatia R, Drakeley C, Carneiro I, Mwakasungula E, Mwerinde O, Saganda K, Shao J, Kitua A, Olomi R, Greenwood BM, Whitty CJ, 2004. Overdiagnosis of malaria in patients with severe febrile illness in Tanzania: a prospective study. BMJ 329 : 1212.
  2. White NJ, Silamut K, 1989. Rapid diagnosis of malaria. Lancet 1 : 435.
  3. Warhurst DC, Williams JE, 1996. ACP Broadsheet no 148. July 1996. Laboratory diagnosis of malaria. J Clin Pathol 49 : 533–538.
  4. Milne LM, Kyi MS, Chiodini PL, Warhurst DC, 1994. Accuracy of routine laboratory diagnosis of malaria in the United Kingdom. J Clin Pathol 47 : 740–742.
  5. Proux S, Hkirijareon L, Ngamngonkiri C, McConnell S, Nosten F, 2001. Paracheck-Pf: a new, inexpensive and reliable rapid test for P. falciparum malaria. Trop Med Int Health 6 : 99–101.
  6. Moody A, 2002. Rapid diagnostic tests for malaria parasites. Clin Microbiol Rev 15 : 66–78.
  7. Mayxay M, Pukrittayakamee S, Chotivanich K, Looareesuwan S, White NJ, 2001. Persistence of Plasmodium falciparum HRP-2 in successfully treated acute falciparum malaria. Trans R Soc Trop Med Hyg 95 : 179–182.
  8. Tjitra E, Suprianto S, McBroom J, Currie BJ, Anstey NM, 2001. Persistent ICT malaria P.f/P.v panmalarial and HRP2 antigen reactivity after treatment of Plasmodium falciparum malaria is associated with gametocytemia and results in false-positive diagnoses of Plasmodium vivax in convalescence. J Clin Microbiol 39 : 1025–1031.
  9. Snounou G, Viriyakosol S, Zhu XP, Jarra W, Pinheiro L, do Rosario VE, Thaithong S, Brown KN, 1993. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol 61 : 315–320.
  10. Singh B, Bobogare A, Cox-Singh J, Snounou G, Abdullah MS, Rahman HA, 1999. A genus- and species-specific nested polymerase chain reaction malaria detection assay for epidemiologic studies. Am J Trop Med Hyg 60 : 687–692.
  11. Banoo S, Bell D, Bossuyt P, Herring A, Mabey D, Poole F, Smith PG, Sriram N, Wongsrichanalai C, Linke R, O’Brien R, Perkins M, Cunningham J, Matsoso P, Nathanson CM, Olliaro P, Peeling RW, Ramsay A, TDR Diagnostics Evaluation Expert Panel (WHO/TDR), 2006. Evaluation of diagnostic tests for infectious diseases: general principles. Nat Rev Microbiol 4 : S21–S31.
  12. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T, 2000. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28 : E63.
  13. Poon LL, Wong BW, Ma EH, Chan KH, Chow LM, Abeyewickreme W, Tangpukdee N, Yuen KY, Guan Y, Looareesuwan S, Peiris JS, 2006. Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification. Clin Chem 52 : 303–306.
  14. Parida MM, Santhosh SR, Dash PK, Tripathi NK, Lakshmi V, Mamidi N, Shrivastva A, Gupta N, Saxena P, Babu JP, Rao PV, Morita K, 2007. Rapid and real-time detection of Chikungunya virus by reverse transcription loop-mediated isothermal amplification assay. J Clin Microbiol 45 : 351–357.
  15. Poon LL, Leung CS, Chan KH, Lee JH, Yuen KY, Guan Y, Peiris JS, 2005. Detection of human influenza A viruses by loop-mediated isothermal amplification. J Clin Microbiol 43 : 427–430.
  16. Hagiwara M, Sasaki H, Matsuo K, Honda M, Kawase M, Nakagawa H, 2007. Loop-mediated isothermal amplification method for detection of human papillomavirus type 6, 11, 16, and 18. J Med Virol 79 : 605–615.
  17. Parida M, Horioke K, Ishida H, Dash PK, Saxena P, Jana AM, Islam MA, Inoue S, Hosaka N, Morita K, 2005. Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay. J Clin Microbiol 43 : 2895–2903.
  18. Mori Y, Nagamine K, Tomita N, Notomi T, 2001. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun 289 : 150–154.
  19. Landis K, 1977. The measurement of observer agreement for categorical data. Biometrics 33 : 159–174.
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2007.77.972
Loading
/content/journals/10.4269/ajtmh.2007.77.972
Loading

Data & Media loading...

Supplementary Data

Erratum

  • Received : 02 Apr 2007
  • Accepted : 20 Jul 2007

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error