1921
Volume 77, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

We undertook a prospective study to quantitate in blood, pus, respiratory secretions, and urine obtained from 414 patients with melioidosis. The median was count 1.1, 1.5 × 10, 1.1 × 10, and 1.1 × 10 CFU/mL in these sample types, respectively. This provides important insights into the likely feasibility of future studies such as expression microarray analysis using clinical material.

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2007-11-01
2017-09-25
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References

  1. Cheng AC, Currie BJ, 2005. Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev 18 : 383–416.
  2. Wiersinga WJ, van der Poll T, White NJ, Day NP, Peacock SJ, 2006. Melioidosis: insights into the pathogenicity of Burkholderia pseudomallei. Nat Rev Microbiol 4 : 272–282.
  3. Chaowagul W, White NJ, Dance DA, Wattanagoon Y, Naigowit P, Davis TM, Looareesuwan S, Pitakwatchara N, 1989. Melioidosis: a major cause of community-acquired septicemia in northeastern Thailand. J Infect Dis 159 : 890–899.
  4. White NJ, 2003. Melioidosis. Lancet 361 : 1715–1722.
  5. Tuanyok A, Tom M, Dunbar J, Woods DE, 2006. Genome-wide expression analysis of Burkholderia pseudomallei infection in a hamster model of acute melioidosis. Infect Immun 74 : 5465–5476.
  6. Simpson AJ, Howe PA, Wuthiekanun V, White NJ, 1999. A comparison of lysis centrifugation, pour plate, and conventional blood culture methods in the diagnosis of septicaemic melioidosis. J Clin Pathol 52 : 616–619.
  7. Wuthiekanun V, Desakorn V, Wongsuvan G, Amornchai P, Cheng AC, Maharjan B, Limmathurotsakul D, Chierakul W, White NJ, Day NP, Peacock SJ, 2005. Rapid immunofluorescence microscopy for diagnosis of melioidosis. Clin Diagn Lab Immunol 12 : 555–556.
  8. Wuthiekanun V, Dance DA, Wattanagoon Y, Supputtamongkol Y, Chaowagul W, White NJ, 1990. The use of selective media for the isolation of Pseudomonas pseudomallei in clinical practice. J Med Microbiol 33 : 121–126.
  9. Chantratita N, Wuthiekanun V, Boonbumrung K, Tiyawisutsri R, Vesaratchavest M, Limmathurosakul D, Chierakul W, Wongratanacheewin S, Pukritiyakamee S, White NJ, Day NP, Peacock SJ, 2007. Biological relevance of colony morphology and phenotypic switching by Burkholderia pseudomallei. J Bacteriol 189 : 807–812.
  10. Anuntagool N, Naigowit P, Petkanchanapong V, Aramsri P, Panichakul T, Sirisinha S, 2000. Monoclonal antibody-based rapid identification of Burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia. J Med Microbiol 49 : 1075–1078.
  11. Wuthiekanun V, Anuntagool N, White NJ, Sirisinha S, 2002. Short report: a rapid method for the differentiation of Burkholderia pseudomallei and Burkholderia thailandensis. Am J Trop Med Hyg 66 : 759–761.
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Supplementary Data

Erratum

  • Received : 18 May 2007
  • Accepted : 19 Jul 2007

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