Volume 77, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


A multiplex real-time PCR was developed and evaluated for the simultaneous detection of , , and in fecal samples. Using well-defined control samples ( = 150), known positive fecal samples ( = 50), and fecal samples from an area in Ghana where human infections with all 3 nematode species are endemic ( = 339), the method proved to be highly specific and sensitive. Cycle threshold (Ct) values, reflecting parasite-specific DNA load, showed significant correlation with the intensity of infection as measured by microscopy using Kato–Katz fecal smears or by species specific third-stage larval count after coproculture. The multiplex real-time PCR described combined with the simple fecal sample collection procedure and the potential for high throughput makes this approach a powerful diagnostic tool to study species-specific transmission patterns of human hookworm-like infections. Moreover, this procedure facilitates monitoring of intervention programs and allows species-specific detection of treatment failure following rounds of mass treatment.


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  • Received : 21 Dec 2006
  • Accepted : 03 Jun 2007

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