Volume 77, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


In North America, West Nile and St. Louis encephalitis viruses have been detected in a wide range of vector species, but the majority of isolations continue to be from pools of mixed mosquitoes in the subgenus . Unfortunately, the morphologic identification of these important disease vectors is often difficult, particularly in regions of sympatry. We developed a sensitive real-time TaqMan polymerase chain reaction assay that allows reliable identification of mosquitoes including , , , and . Primers and fluorogenic probes specific to each species were designed based on sequences of the gene (). Both immature and adult mosquitoes were successfully identified as individuals and as mixed species pools. This identification technique provides the basis for a rapid, sensitive, and high-throughput method for expounding the species-specific contribution of vectors to various phases of arbovirus transmission.


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  • Received : 31 Oct 2006
  • Accepted : 25 Mar 2007

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