1921
Volume 77, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

A total of 271 stool specimens were collected from children (diarrheagenic, n = 115 and control, n = 54) and adults (diarrheagenic, n = 73 and control, n = 29) from Tunis, Tunisia, and processed to detect bacterial enteropathogens, parasites, and viruses. Diarrheagenic (DEC) were identified by their virulence genes (polymerase chain reaction) and adherence patterns (tissue culture assays). The most frequently isolated enteric pathogens from diarrheagenic children were enterotoxigenic (ETEC, 32.3%), enteroaggregative (EAEC, 11.3%), enteroinvasive (EIEC, (11.3%), adenovirus (10.4%), enterohemorrhagic (EHEC, 10.4%), and spp. (9.5%). For children in the control group, ETEC (37%), EAEC (15%), EHEC (11.1%), and typical enteropathogenic (EPEC, 11.1%) were the most common enteric pathogens. In adults in the diarrheagenic group, spp. (34.2%), ETEC (12.3%), adenovirus (7%), and spp. (4%) were the most common enteric pathogens. In adults in the control group, ETEC (31%) was the most common enteric pathogen. Multiple pathogens were recovered from 22% of the diarrheagenic children and 7% of the diarrheagenic adults. strains showed high resistance rates to tetracycline, streptomycin, and β-lactams. The most frequent combinations were ETEC-rotavirus and ETEC-adenovirus. Pulsed-field gel electrophoresis for DEC indicated a large number of DEC clones (five major clones) persistent in the community reservoir for a considerable period of time that caused diarrhea in the population. This suggests the confluence of small epidemics by clonally related DEC strains circulating in this region.

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  • Received : 04 Aug 2006
  • Accepted : 08 Jan 2007

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