1921
Volume 77, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

The principal malaria vector in Africa, contains two pairs of autosomes and one pair of sex chromosomes. The Y chromosome is only associated with males and other Y chromosome–specific DNA sequences, which are transferred to women during mating. A reliable tool to determine the mating status of dried wild females is currently lacking. DNA was extracted from dried virgin and mated females and used to test whether Y chromosome–specific polymerase chain reaction (PCR) markers can be successfully amplified and used as a predictor of mating. Here we report a new PCR-based method to determine the mating status among successfully inseminated and virgin wild females, using three male-specific primers. This dissection-free method has the potential to facilitate studies of both population demographics and gene flow from dried mosquito samples routinely collected in epidemiologic monitoring and aid existing and new malaria-vector control approaches.

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2007-09-01
2017-11-25
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References

  1. Muller HJ, 1932. Some genetic aspects of sex. Am Nat 66 : 118–138.
  2. Charlesworth B, Charlesworth D, 2000. The degeneration of Y chromosomes. Philos Trans R Soc Lond B Biol Sci 355 : 1563– 1572.
  3. White MJD, 1973. Animal Cytology and Evolution. Cambridge: University Press.
  4. Skaletsky H, Kuroda-Kawaguchi T, Minx PJ, Cordum HS, Hillier L, 2003. The male-specific region of the human Y chromosome is a mosaic of discrete sequencie classes. Nature 423 : 825–837.
  5. Bachtrog D, 2003. Accumulation of spock and worf, two novel non-LTR retrotransposons on the neo Y-chromosome of Drosophila miranda. Mol Biol Evol 20 : 173–181.
  6. Bachtrog D, 2003. Adaptation shapes pattern of genome evolution on sexual and asexual chromosomes in Drosophila. Nat Genet 34 : 215–219.
  7. Steinemann S, Steinemann M, 2000. Y chromosomes: born to be destroyed. Bioessays 27 : 1076–1083.
  8. Carvalho AB, Lazzaro BP, Clack AG, 2000. Y-chromosomal fertility factors kl-2 and kl-3 of Drosophila melanogaster encode dynein havy chain polypeptides. Proc Natl Acad Sci USA 98 : 13225–13230.
  9. Carvalho AB, Dobo BA, Vibranovski MD, Clack AG, 2001. Identification of five new genes on the Y-chromosome of Drosophila melanogaster genome: how far can we go? Genetica 117 : 227–237.
  10. Steinemann M, Steinemann S, 1998. Enigma of Y-chromosome degeneration: neo-Y and neo-X chromosomes of Drosophila miranda a model for sex chromosome evolution. Genetica 102/103 : 409–420.
  11. Krzywinski J, Nusskern DR, Kern MK, Besansky NJ, 2004. Isolation and Characterization of Y chromosome sequences from the African malaria mosquito Anopheles gambiae. Genetics 166 : 1291–1302.
  12. Krzywinski J, Sangare D, Besansky NJ, 2005. Satellite DNA from the Y-chromosome of the malaria vector Anopheles gambiae. Genetics 169 : 185–196.
  13. Clements AN, 1992. The Biology of Mosquitoes, Vol 1: Development, Nutrition and Reproduction. London: Chapmann and Hall.
  14. Chomczynski P, Mackey K, Drews R, Wilfinger W, 1997. DNA-zol: a reagent for the rapid isolation of genomic DNA. Bio-techniques 22 : 550–553.
  15. Tabachnick WJ, 2003. Reflections on the Anopheles gambiae genome sequence, transgenic mosquitoes and the prospect for controlling malaria and other vector borne diseases. J Med Entomol 40 : 597–606.
  16. Catteruccia F, Godfray HCJ, Crisanti A, 2003. Impact of genetic manipulation on the fitness of Anopheles stephensi mosquitoes. Science 299 : 1225–1227.
  17. Knols BGJ, Njiru BNN, Mathenge EM, Mukabana WR, Beier JC, Killeen GF, 2002. Malariasphere: a greenhouse-enclosed simulation of a natural Anopheles gambiae (Diptera: Culi-cidae) ecosystem in Western Kenya. Malar J 1 : 19.
  18. Ferguson FM, John B, Ng’habi KR, Knols BGJ, 2005. Addressing the sex imbalance in knowledge of vector biology. Trends Ecol Evol 20 : 202–209.
  19. Knols BGJ, Njiru BNN, Mukabana RW, Mathenge EM, Killeen GF, 2003. Contained semi-field environments for ecological studies on transgenic African malaria vectors. Scott TW, Takken W, eds. Ecology of Transgenic Mosquitoes. Wageningen: Wageningen University and Research Centre, 99–106.
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  • Received : 31 Jan 2007
  • Accepted : 30 Apr 2007

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