Volume 77, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


We developed a molecular assay to detect predation on (s.l.) mosquitoes. This intergenic spacer ribosomal DNA polymerase chain reaction assay and restriction enzyme analysis uses -specific primers to detect mosquito DNA in the DNA extracts from whole invertebrate predators, which enables identification of species ( s.s. versus ) and molecular forms (M versus S in s.s.). We show that s.l. DNA can be detected after ingestion by members of the families Lestidae (order Odonata) after four hours, Libellulidae (order Odonata) after six hours, and Notonectidae (order Hemiptera) after 24 hours. This method is an improvement over previously published methods because of ease of execution and increased time of detection after ingestion.


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  • Received : 19 Sep 2006
  • Accepted : 08 May 2007
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