1921
Volume 77, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

We developed a molecular assay to detect predation on (s.l.) mosquitoes. This intergenic spacer ribosomal DNA polymerase chain reaction assay and restriction enzyme analysis uses -specific primers to detect mosquito DNA in the DNA extracts from whole invertebrate predators, which enables identification of species ( s.s. versus ) and molecular forms (M versus S in s.s.). We show that s.l. DNA can be detected after ingestion by members of the families Lestidae (order Odonata) after four hours, Libellulidae (order Odonata) after six hours, and Notonectidae (order Hemiptera) after 24 hours. This method is an improvement over previously published methods because of ease of execution and increased time of detection after ingestion.

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2007-09-01
2017-11-20
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References

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  8. Santolamazza F, Della Torre A, Caccone A, 2004. Short report: a new polymerase chain reaction–restriction fragment length polymorphism method to identify Anopheles arabiensis from Anopheles gambiae and its two molecular forms from degraded DNA templates or museum samples. Am J Trop Med Hyg 70 : 604–606.
  9. Morales ME, Wesson DM, Sutherland IW, Impoinvil DE, Mbogo CM, Githure JI, Beier JC, 2003. Determination of Anopheles gambiae larval DNA in the gut of insectivorous dragonfly (Libellulidae) nymphs by polymerase chain reaction. J Am Mosq Control Assoc 19 : 163–165.
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  • Received : 19 Sep 2006
  • Accepted : 08 May 2007

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