Volume 77, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Surveillance of the prevalence of and its vector, sand fly species, in endemic and surrounding areas is important for prediction of the risk and expansion of leishmaniasis. In this study, a method for the mass screening of sand flies for infection was established. This method was applied to 319 field-captured specimens, and 5 positive sand flies were detected. Sand fly species were identified by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) of the18S rRNA gene, and all the positive flies were . Furthermore, cytochrome ( b) gene sequence analyses identified all the parasites as species including a probable novel species. Because the method requires minimum effort and can process a large number of samples at once, it will be a powerful tool for studying the epidemiology of leishmaniasis.


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  • Received : 13 Feb 2007
  • Accepted : 01 May 2007

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