Volume 77, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


No studies have addressed the spatial complexity of populations in Zambia or the effects of drought on the genetic structure of this species. We genotyped approximately 420 at 12 microsatellite loci representing 18 collections from the Southern Province of Zambia. Collections spanned three transmission seasons and covered a wet year–drought year–wet year cycle. within the 2,000 km of the Macha study region were panmictic, with high gene flow between Macha and Namwala, Zambia, which are 80 km apart. There was little evidence for genetic structuring among years, with no significant shifts in allele frequency distributions or observed heterozygosity, and no evidence for a genetic bottleneck despite a drastic reduction in mosquito numbers during the drought year. in southern Zambia has a large deme size, and the regional genetic structure of this species was little affected by an extended drought period.


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  1. Gillies MT, Coetzee M, 1987. A Supplement to the Anophelinae of Africa South of the Sahara. Johannesburg: South African Institute for Medical Research Publication no. 55.
  2. Coetzee M, Craig M, Le Sueur D, 2000. Distribution of African malaria mosquitoes belonging to the Anopheles gambiae complex. Parasitol Today 16 : 74–77.
  3. Chandre F, Manguin S, Brengues C, Yovo JD, Darriet F, Diabate A, Carnevale P, Guillet P, 1999. Current distribution of a pyrethroid resistance gene (kdr) in Anopheles gambiae complex from west Africa and further evidence for reproductive isolation of the Mopti form. Parassitologia 41 : 319–322.
  4. Weill M, Chandre F, Brengues C, Manguin S, Akogbeto M, Pasteur N, Guillet P, Raymond M, 2000. The kdr mutation occurs in the Mopti form of Anopheles gambiae s.s. through introgression. Insect Mol Biol 9 : 451–455.
  5. Diabaté A, Baldet T, Chandre F, Dabire KR, Simard F, Ouedraogo JB, Guillet P, Hougard JM, 2004. First report of a kdr mutation in Anopheles arabiensis from Burkina Faso, west Africa. J Am Mosq Control Assoc 20 : 195–196.
  6. James AA, Beerntsen BT, Capurro Mde L, Coates CJ, Coleman NJ, Jasinskiene N, Krettli AU, 1999. Controlling malaria transmission with genetically-engineered, Plasmodium-resistant mosquitoes: milestones in a model system. Parassitologia 41 : 461–471.
  7. Tripet F, Dolo G, Lanzaro GC, 2005. Multilevel analyses of genetic differentiation in Anopheles gambiae s.s. reveal patterns of gene flow important for malaria-fighting mosquito projects. Genetics 169 : 313–324.
  8. Lanzaro GC, Touré YT, Carnahan J, Zheng L, Dolo G, Traoré S, Petrarca V, Vernick KD, Taylor CE, 1998. Complexities in the genetic structure of Anopheles gambiae populations in west Africa as revealed by microsatellite DNA analysis. Proc Natl Acad Sci U S A 95 : 14260–14265.
  9. Coluzzi M, Sabatini A, della Torre A, Di Deco MA, Petrarca V, 2002. A polytene chromosome analysis of the Anopheles gambiae species complex. Science 298 : 1415–1418.
  10. Zheng L, Benedict MQ, Cornel AJ, Collins FH, Kafatos FC, 1996. An integrated genetic map of the African human malaria vector mosquito, Anopheles gambiae. Genetics 143 : 941–952.
  11. Lehmann T, Hawley WA, Kamau L, Fontenille D, Simard F, Collins FH, 1996. Genetic differentiation of Anopheles gambiae populations from east and west Africa: comparison of microsatellite and allozyme loci. Heredity 77 : 192–200.
  12. Lehmann T, Licht M, Elissa N, Maega BTA, Chimumbwa JM, Watsenga FT, Wondji CS, Simard F, Hawley WA, 2003. Population structure of Anopheles gambiae in Africa. J Hered 94 : 133–147.
  13. Carnahan J, Zheng L, Taylor CE, Toure YT, Norris DE, Dolo G, Diuk-Wasser M, Lanzaro GC, 2002. Genetic differentiation of Anopheles gambiae s.s. populations in Mali, west Africa, using microsatellite loci. J Hered 93 : 249–253.
  14. Taylor C, Touré YT, Carnahan J, Norris DE, Dolo G, Traoré SF, Edillo FE, Lanzaro GC, 2001. Gene flow among populations of the malaria vector Anopheles gambiae, in Mali, west Africa. Genetics 157 : 743–750.
  15. Simard F, Lehmann T, Lemasson J-J, Diatta M, Fontenille D, 2000. Persistance of Anopheles arabiensis during the severe dry season conditions in Senegal: an indirect approach using microsatellite loci. Insect Mol Biol 9 : 467–479.
  16. Donnelly MJ, Cuamba N, Charlwood JD, Collins FH, Townson H, 1999. Population structure in the malaria vector, Anopheles arabiensis Patton, in east Africa. Heredity 83 : 408–417.
  17. Donnelly MJ, Townson H, 2000. Evidence for extensive genetic differentiation among populations of the malaria vector Anopheles arabiensis in eastern Africa. Insect Mol Biol 9 : 357–367.
  18. Simard F, Fontenille D, Lehmann T, Girod R, Brutus L, Gopaul R, Dournon C, Collins FH, 1999. High amounts of genetic differentiation between populations of the malaria vector Anopheles arabiensis from west Africa and eastern outer islands. Am J Trop Med Hyg 60 : 1000–1009.
  19. Temu EA, Yan G, 2005. Microsatellite and mitochondrial genetic differentiation of Anopheles arabiensis (Diptera: Culicidae) from western Kenya, the Great Rift Valley, and coastal Kenya. Am J Trop Med Hyg 73 : 726–733.
  20. Nyanjom SR, Chen H, Gebre-Michael T, Bekele E, Shililu J, Githure J, Beier JC, Yan G, 2003. Population genetic structure of Anopheles arabiensis mosquitoes in Ethiopia and Eritrea. J Hered 94 : 457–463.
  21. Kamau L, Mukabana WR, Hawley WA, Lehmann T, Irungu W, Orago AA, Collins FH, 1999. Analysis of genetic variability in Anopheles arabiensis and Anopheles gambiae using microsatellite loci. Insect Mol Biol 8 : 287–297.
  22. Onyabe DY, Conn JE, 2001. Population genetic structure of the malaria mosquito Anopheles arabiensis across Nigeria suggests range expansion. Mol Ecol 10 : 2577–2591.
  23. Fontenille D, Lochouarn L, Diatta M, Sokhna C, Dia I, Diagne N, Lemasson J-J, Ba K, Tall A, Rogier C, Trape J-F, 1997. Four years’ entomological study of the transmission of seasonal malaria in Senegal and the bionomics of Anopheles gambiae and An. arabiensis. Trans R Soc Trop Med Hyg 91 : 647–652.
  24. Shililu J, Ghebremeskel T, Mengistu S, Fekadu H, Zerom M, Mbogo C, Githure J, Novak R, Brantly E, Beier JC, 2003. High seasonal variation in entomologic inoculation rates in Eritrea, a semi-arid region of unstable malaria in Africa. Am J Trop Med Hyg 69 : 607–613.
  25. Kent RJ, Thuma PE, Mharakurwa S, Norris DE, 2007. Seasonality, blood feeding behavior, and transmission of Plasmodium falciparum by Anopheles arabiensis following an extended drought in southern Zambia. Am J Trop Med Hyg 76 : 267–274.
  26. Adams PC, 1940. Some observations on the flight of stained anophelines at Nkana, northern Rhodesia. Ann Trop Med Parasitol 34 : 35–43.
  27. Thompson MC, Connor SJ, Quinones ML, Jawara M, Todd J, Greenwood BM, 1995. Movement of Anopheles gambiae s.l. malaria vectors between villages in The Gambia. Med Vet Entomol 9 : 413–419.
  28. Okello PE, Van Bortel W, Byaruhanga AM, Correwyn A, Roelants P, Talisuna A, D’Alessandro U, Coosemans M, 2006. Variation in malaria transmission intensity in seven sites throughout Uganda. Am J Trop Med Hyg 75 : 219–225.
  29. Ralisoa Randrianasolo BO, Coluzzi M, 1987. Genetical investigations on zoophilic and exophilic Anopheles arabiensis from Antananarivo area (Madagascar). Parassitologia 29 : 93–97.
  30. Ameneshewa B, Service MW, 1996. Resting habits of Anopheles arabiensis in the Awash river valley of Ethiopia. Ann Trop Med Parasitol 90 : 515–521.
  31. Coluzzi M, Sabatini A, Petrarca V, Di Deco MA, 1979. Chromosomal differentiation and adaptation to human environments in the Anopheles gambiae complex. Trans R Soc Trop Med Hyg 73 : 483–497.
  32. Touré YT, Petrarca V, Traoré SF, Coulibaly A, Maiga HM, Sankare O, Sow M, Di Deco MA, Coluzzi M, 1998. Distribution and inversion polymorphism of chromosomally recognized taxa of the Anopheles gambiae complex in Mali, West Africa. Parassitologia 40 : 477–511.
  33. Larkin GL, Thuma PE, 1991. Congenital malaria in a hyperendemic area. Am J Trop Med Hyg 45 : 587–592.
  34. FEWS NET, Food Security Update, February 2005. US Agency for International Development. March 14, 2005. Available from http://www.fews.net/centers/?f=zm
  35. Sudia WD, Chamberlain RW, 1962. Battery operated light trap, an improved model. Mosq News 22 : 126–129.
  36. Gillies MT, DeMeillon B, 1968. The Anophelinae of Africa South of the Sahara (Ethiopian Zoogeographical Region). Second edition. Johannesburg: South African Institute for Medical Research. Publication no. 54.
  37. Kent RJ, Norris DE, 2005. Identification of mammalian blood meals in mosquitoes by a multiplexed polymerase chain reaction targeting cytochrome b. Am J Trop Med Hyg 73 : 336–342.
  38. Simon C, Frati F, Beckenbach A, Crespi B, Liu H, Flook P, 1994. Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann Entomol Soc Am 87 : 651–701.
  39. Scott JA, Brogdon WG, Collins FH, 1993. Identification of single specimens of the Anopheles gambiae complex by the polymerase chain reaction. Am J Trop Med Hyg 49 : 520–529.
  40. Schneider S, Kueffer J-M, Roessli D, Excoffier L, 1997. Arlequin Version 1.1: A Software for Population Genetic Data Analysis. Geneva: Genetics and Biometry Laboratory, University of Geneva.
  41. Weir BS, Cockerham CC, 1984. Estimating F-statistics for the analysis of population structure. Evolution Int J Org Evolution 38 : 1358–1370.
  42. Slatkin M, 1995. A measure of population subdivision based on microsatellite allele frequencies. Genetics 139 : 457–462.
  43. Guo SW, Thompson EA, 1992. Performing the exact test of Hardy-Weinberg proportion for multiple alleles. Biometrics 48 : 361–372.
  44. Chakraborty R, De Andrade M, Daiger SP, Budowle B, 1992. Apparent heterozygote deficiencies observed in DNA typing data and their implications in forensic applications. Ann Hum Genet 56 : 45–57.
  45. Slatkin M, Excoffier L, 1996. Testing for linkage disequilibrium in genotypic data using the EM algorithm. Heredity 76 : 377–383.
  46. Hartl DL, Clark AG, eds. Principles of Population Genetics. Second edition. Sunderland, MA: Sinauer Associates.
  47. Cornuet JM, Luikart G, 1996. Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data. Genetics 144 : 2001–2014.
  48. Forbes SH, Hogg JT, Buchanan FC, Crawford AM, Allendorf FW, 1995. Microsatellite evolution in congenereic mammals: domestic and bighorn sheep. Mol Biol Evol 12 : 1106–1113.
  49. White GB, 1974. Anopheles gambiae complex and disease transmission in Africa. Trans R Soc Trop Med Hyg 68 : 278–301.
  50. Donnelly MJ, Pinto J, Girod R, Besansky NJ, Lehmann T, 2004. Revisiting the role of introgression vs shared ancestral polymorphisms as key processes shaping genetic diversity as recently separated sibling species of the Anopheles gambiae complex. Heredity 92 : 61–68.
  51. Thelwell NJ, Huisman RA, Harbach RE, Butlin RK, 2000. Evidence for mitochondrial introgression between Anopheles bwambe, and Anopheles gambiae. Insect Mol Biol 9 : 203–210.
  52. Petrarca V, Nugud AD, Elkarim Ahmed MA, Haridi AM, Di Deco MA, Coluzzi M, 2000. Cytogenetics of the Anopheles gambiae complex in Sudan, with special reference to An. arabiensis: relationships with east and west African populations. Med Vet Entomol 14 : 149–164.
  53. Mnzava AEP, Rwegoshora RT, Wilkes TJ, Tanner M, Curtis CF, 1995. Anopheles arabiensis and An. gambiae chromosomal inversion polymorphism, feeding and resting behaviour in relation to insecticide house-spraying in Tanzania. Med Vet Entomol 9 : 316–324.
  54. Taylor CE, Toure YT, Coluzzi M, Petrarca V, 1993. Effective population size and persistence of Anopheles arabiensis during the dry season in west Africa. Med Vet Entomol 7 : 351–357.
  55. Constantini C, Li SG, Della Torre A, Sagnon N’F, Coluzzi M, Taylor CE, 1996. Density, survival, and dispersal of Anopheles gambiae complex mosquitoes in a west African Sudan savanna village. Med Vet Entomol 10 : 203–219.
  56. Lehmann T, Hawley WA, Grebert H, Collins FH, 1998. The effective population size of Anopheles gambiae in Kenya: implications for population structure. Mol Biol Evol 15 : 264–276.

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Supplementary Table 1

  • Received : 06 Dec 2006
  • Accepted : 08 May 2007

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