Volume 77, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Dengue virus (DENV) is transmitted to humans by mosquitoes of the genus . Although several molecules have been described as part of DENV receptor complex in mosquito cells, none of them have been identified. Our group characterized two glycoproteins (40 and 45 kD) as part of the DENV receptor complex in C6/36 cells. Because identification of the mosquito cell receptor has been unsuccessful and some cell receptors described for DENV in mammalian cells are heat-shock proteins (HSPs), the role of HSPs in DENV binding and infection in C6/36 cells was evaluated. Our results indicate that gp45 and a 74-kD molecule (p74), which interact with DENV envelope protein, are immunologically related to HSP90. Although p74 is induced by heat shock, gp45 apparently is not. However, these proteins are relocated to the cell surface after heat-shock treatment, causing an increase in virus binding without any effect on virus yield.


Article metrics loading...

The graphs shown below represent data from March 2017
Loading full text...

Full text loading...



  1. Guzman MG, Kouri G, 2002. Dengue: an update. Lancet Infect Dis 2 : 33–42. [Google Scholar]
  2. Jacobs M, 2000. Dengue: emergence as a global public health problem and prospects for control. Trans R Soc Trop Med Hyg 94 : 7–8. [Google Scholar]
  3. Ramos C, 1989. Biología de la Infección causada por el virus del dengue. Salud Publica Mex 31 : 100–105. [Google Scholar]
  4. Reinert JF, Harbach RE, 2005. Generic and subgeneric status of aedine mosquito species (Diptera: Culicidae: Aedini) occurring in the Australasian region. Zootaxa 887 : 1–10. [Google Scholar]
  5. Salas-Benito JS, del Angel RM, 1997. Identification of two surface proteins from C6/36 cells that bind dengue type 4 virus. J Virol 71 : 7246–7252. [Google Scholar]
  6. Igarashi A, 1978. Isolation of a Singh’s Aedes albopictus cell clone sensitive to dengue and chikungunya viruses. J Gen Virol 40 : 531–544. [Google Scholar]
  7. Mendoza YM, Salas-Benito JS, Lanz-Mendoza H, Hernandez-Martinez S, del Angel RM, 2002. A putative receptor for dengue virus in mosquito tissues: localization of a 45-kDa glyco-protein. Am J Trop Med Hyg 67 : 76–84. [Google Scholar]
  8. Triantafilou K, Fradelizi D, Wilson K, Triantafilou M, 2002. GRP78, a coreceptor for coxsackievirus A9, interacts with major histocompatibility complex class I molecules which mediate virus internalization. J Virol 76 : 633–643. [Google Scholar]
  9. Guerrero CA, Bouyssounade D, Zárate S, Isa P, López T, Espinosa R, Romero P, Méndez E, López S, Arias CF, 2002. Heat shock cognate protein 70 is involved in rotavirus cell entry. J Virol 76 : 4096–4102. [Google Scholar]
  10. Zárate S, Cuadras MA, Espinosa R, Romero P, Juárez KO, Camacho-Nuez M, Arias CF, López S, 2003. Interaction of rotaviruses with Hsc70 during cell entry is mediated by VP5. J Virol 77 : 7254–7260. [Google Scholar]
  11. Reyes del Valle J, Chavez-Salinas S, Medina F, del Angel RM, 2005. Heat shock protein 90 and heat shock protein 70 are components of dengue virus receptor complex in human cells. J Virol 79 : 4547–4567. [Google Scholar]
  12. Jindadamrongwech S, Thepparit C, Smith DR, 2004. Identification of GRP 78 (BiP) as a liver cell expressed receptor element for dengue virus serotype 2. Arch Virol 149 : 915–927. [Google Scholar]
  13. Craig EA, Gross CA, 1991. Is hsp70 the cellular thermometer? Trends Biochem Sci 16 : 135–140. [Google Scholar]
  14. Lindquist S, Craig EA, 1988. The heat shock proteins. Annu Rev Genet 22 : 631–677. [Google Scholar]
  15. Gould EA, Clegg JCS, 1991. Growth, titration and purification of alphaviruses and flaviviruses. Mahy BWJ, editor. Virology: A Practical Approach. Oxford, United Kingdom: IRL Press, 43–78.
  16. Kuno G, Oliver A, 1989. Maintaining mosquito cell lines at high temperatures: effects on the replication of flaviviruses. In Vitro Cell Dev Biol 25 : 193–196. [Google Scholar]
  17. Reyes del Valle J, del Angel RM, 2004. Isolation of putative dengue virus receptor molecules by affinity chromatography using a recombinant E protein ligand. J Virol Methods 116 : 95–102. [Google Scholar]
  18. Triantafilou K, Triantafilou M, 2004. Coxsackievirus B4-induced cytokine production in pancreatic cells is mediated through toll-like receptor 4. J Virol 78 : 11313–11320. [Google Scholar]
  19. Riehl RM, Sullivan WP, Vroman BT, Bauer VJ, Pearson GR, Toft, DO, 1985. Immunological evidence that the nonhormone binding component of avian steroid receptors exists in a wide range of tissues and species. Biochemistry 24 : 6586–6591. [Google Scholar]
  20. Mercado-Curiel RF, Esquinca-Avilés HA, Tovar R, Díaz-Badillo A, Camacho-Nuez M, Muñoz ML, 2006. The four serotypes of dengue recognize the same putative receptors in Aedes aegypti midgut and Ae. albopictus cells. BMC Microbiol 6 : 85–95. [Google Scholar]
  21. Chu JJ, Leong PW, Ng ML, 2005. Characterization of plasma membrane-associated proteins from Aedes albopictus mosquito (C6/36) cells that mediate West Nile virus binding and infection. Virology 339 : 249–260. [Google Scholar]
  22. Muñoz ML, Cisneros A, Cruz J, Das P, Tovar R, Ortega A, 1988. Putative dengue virus receptors from mosquito cells. FEMS Microbiol Lett 168 : 251–258. [Google Scholar]
  23. Sakoonwatanyoo P, Boonsanay V, Smith DR, 2006. Growth and production of the dengue virus in C6/36 cells and identification of a laminin-binding protein as a candidate serotype 3 and 4 receptor protein. Intervirology 49 : 161–172. [Google Scholar]
  24. Gilman A, 1987. G proteins: transducers of receptor-generated signals. Annu Rev Biochem 56 : 615–649. [Google Scholar]
  25. Pearl LH, Prodromou C, 2006. Structure and mechanism of the Hsp90 molecular chaperone machinery. Annu Rev Biochem 75 : 271–295. [Google Scholar]
  26. Sales K, Brandt W, Rumbak E, Lindsey G, 2000. The LEA-like protein HSP 12 in Saccharomyces cerevisiae has a plasma membrane location and protects membranes against desiccation and ethanol-induced stress. Biochim Biophys Acta 1463 : 267–278. [Google Scholar]
  27. Trent JD, Kagawa HK, Paavola CD, McMillan RA, Howard J, Jahnke L, Lavin C, Embaye T, Henze CE, 2003. Intracellular localization of a group II chaperonin indicates a membrane-related function. Proc Natl Acad Sci USA 100 : 15589–15594. [Google Scholar]
  28. Pfister G, Stroh CM, Perschinka H, Kind M, Knoflach M, Hinterdorfer P, Wick G, 2005. Detection of HSP60 on the membrane surface of stressed human endothelial cells by atomic force and confocal microscopy. J Cell Sci 118 : 1587–1594. [Google Scholar]
  29. Gakhar SK, Shandilya H, 1999. Heat shock response during development of the malaria vector Anopheles stephensi (Culicidae: Diptera). Cytobios 99 : 173–182. [Google Scholar]
  30. Mourya DT, Yadav P, Mishra AC, 2004. Effect of temperature stress on immature stages and susceptibility of Aedes aegypti mosquitoes to Chikungunya virus. Am J Trop Med Hyg 70 : 346–350. [Google Scholar]
  31. Cripe T, Delos PE, Estes PA, Garcea RL, 1995. In vivo and in vitro association of Hsc70 with polyoma virus capsid proteins. J Virol 69 : 7807–7813. [Google Scholar]
  32. Florin L, Becker KA, Sapp C, Lambert C, Sirma H, Müller M, Streeck RE, Sapp M, 2004. Nuclear translocation of papillomavirus minor capsid protein L2 requires Hsc70. J Virol 78 : 5546–5553. [Google Scholar]
  33. Jindal S, Young RA, 1992. Vaccinia virus infection induce stress response that leads to association of hsp70 with viral proteins. J Virol 66 : 5357–5362. [Google Scholar]
  34. Macejak DG, Sarnow P, 1992. Association of heat shock protein 70 with enterovirus capsid precursor P1 in infected human cell. J Virol 66 : 1520–1527. [Google Scholar]
  35. Ohgitani E, Kobayashi K, Takeshita K, Imanishi J, 1999. Biphasic translocation of a 70 kDa heat shock protein in human cytomegalovirus infected cells. J Gen Virol 80 : 63–68. [Google Scholar]
  36. Prange R, Werr M, Loffler-Mary M, 1999. Chaperones involved in hepatitis B virus morphogenesis. Biol Chem 380 : 305–314. [Google Scholar]
  37. Sagara Y, Ishida C, Inouse Y, Shiraki H, Maeda Y, 1998. 71-kilodalton heat shock cognate protein acts as cellular receptor for syncytium formation induced by human T cell lymphotropic virus type 1. J Virol 72 : 535–541. [Google Scholar]
  38. Speth C, Prohaszka M, Mair M, Stockl G, Zhu X, Jobstl B, Gerace L, 1999. A 60 kDa heat shock protein-like molecule interacts with the HIV transmembrane glycoprotein gp41. Mol Immunol 36 : 619–623. [Google Scholar]

Data & Media loading...

  • Received : 09 Jul 2006
  • Accepted : 27 Apr 2007

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error