1921
Volume 77, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

The merozoite surface protein-1 (MSP-1) is a blood stage antigen currently being tested as a vaccine against malaria. Determining the MSP-1 haplotype(s) present during infection is essential for assessments of MSP-1 vaccine efficacy and studies of protective immunity in human populations. The C-terminal fragment (MSP-1) has four predominant haplotypes based on point mutations resulting in non-synonymous amino acid changes: E-TSR (PNG-MAD20 type), E-KNG (Uganda-PA type), Q-KNG (Wellcome type), and Q-TSR (Indo type). Current techniques using direct DNA sequencing are laborious and expensive. We present an MSP-1 allele-specific polymerase chain reaction (PCR)/ligase detection reaction–fluorescent microsphere assay (LDR-FMA) that allows simultaneous detection of the four predominant MSP-1 haplotypes with a sensitivity and specificity comparable with other molecular methods and a semi-quantitative determination of haplotype contribution in mixed infections. Application of this method is an inexpensive, accurate, and high-throughput alternative to distinguish the predominant MSP-1 haplotypes in epidemiologic studies.

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2007-08-01
2017-09-23
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  • Received : 02 Jan 2007
  • Accepted : 27 Apr 2007

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