Volume 77, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Artificial membrane feeding (AMF) assays are used to determine malaria transmission-blocking activity in . The purpose of this study was to determine the effect of the most widely used anticoagulants, EDTA and heparin, on development of the sporogonic cycle. Blood samples collected from 60 patients carrying infections were used to feed using AMF. Seven days after feeding, mosquitoes were dissected to assess mosquito infection. Mosquitoes fed with blood containing EDTA showed a lower mean oocyst number as compared with those fed blood with heparin. However, this effect was minimized upon reduction of EDTA concentrations in the serum. This result may be explained by the fact that microgametocytes require Ca, Mn, and Mg to activate enzymes important for exflagellation process and for motility of ookinetes. We therefore recommend that heparin be used as the anticoagulant of choice for blood used in AMF assays.


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  1. Gonzalez JM, Olano V, Vergara J, Arevalo-Herrera M, Carrasquilla G, Herrera S, Lopez JA, 1997. Unstable, low-level transmission of malaria on the Colombian Pacific Coast. Ann Trop Med Parasitol 91 : 349–358. [Google Scholar]
  2. Robert V, Boudin C, 2003. Biologie de la transmission homme–moustique du Plasmodium. Bull Soc Pathol Exot 96 : 6–20. [Google Scholar]
  3. Kiszewski A, Mellinger A, Spielman A, Malaney P, Ehrlich SS, Sachs J, 2004. A global index representing the stability of malaria transmission. Am J Trop Med Hyg 70 : 486–498. [Google Scholar]
  4. Ranawaka MB, Munesinghe YD, da Silva DMR, Carter R, Mendis KN, 1988. Boosting of transmission blocking immunity during natural Plasmodium vivax infection in humans depends upon frequent reinfection. Infec Immun 56 : 1820–1824. [Google Scholar]
  5. Naotunne TS, Karunaweera ND, Del Giudice G, Kularatne MU, Grau GE, Carter R, Mendis K, 1991. Cytokines kill malaria parasites during infection crisis: extracellular complementary factors are essential. J Exp Med 173 : 523–529. [Google Scholar]
  6. Healer DJ, McGuinness P, Hopcroft SH, Carter R, Riley E, 1997. Complement-mediated lysis of Plasmodium falciparum gametes by malaria-immune human sera is associated with antibodies to the gamete surface antigen Pfs230. Infec Immun 65 : 3017–3023. [Google Scholar]
  7. Kleinschmidt I, Clarke GPY, Bagayoko M, Craig MH, Sueur D, 2000. A spatial statistical approach to malaria mapping. Int J Epidemiol 29 : 355–361. [Google Scholar]
  8. King MV, 1996. Available at: http://www.indstate.edu/thcme/mwking/blood-coagulation.html#image. Accessed November 2006.
  9. Siden-Kiamos I, Ecker A, Nybäck S, Louis C, Sinden RE, Billker O, 2006. Plasmodium berghei calcium-dependent protein kinase 3 is required for ookinete gliding motility and mosquito midgut invasion. Mol Microbiol 60 : 1355–1363. [Google Scholar]
  10. Olano V, Carrasquilla G, Méndez F, 1997. Transmisión de la malaria urbana en Buenaventura, Colombia. Aspectos entomológicos. Rev Panam Salud Públ 1 : 287–294. [Google Scholar]
  11. Salas ML, Romero JF, Solarte Y, Olano V, Herrera MA, Herrera S, 1994. Development of sporogonic cycle of Plasmodium vivax in experimentally infected Anopheles albimanus mosquitoes. Mem Inst Oswaldo Cruz 89 : 115–119. [Google Scholar]
  12. Hurtado S, Salas ML, Romero JF, Zapata JC, Ortiz H, Arevalo-Herrera M, Herrera S, 1997. Regular production of infective sporozoites of Plasmodium falciparum and P. vivax in laboratory-bred Anopheles albimanus. Ann Trop Med Parasitol 91 : 49–60. [Google Scholar]
  13. Social, MP, 2000. Ultimas Normas. Servicio de Salud Colombia. Guias de atención de la malaria (Resolucion Numero 00412 de 2000). Available at: http://www.saludcolombia.com/actual/htmlnormas/ntmalariaI.htm
  14. Arevalo-Herrera M, Solarte Y, Zamora F, Mendez F, Yasnot MF, Rocha L, Long C, Miller LH, Herrera S, 2005. Plasmodium vivax: transmission-blocking immunity in a malaria-endemic area of Colombia. Am J Trop Med Hyg 73 : 38–43. [Google Scholar]
  15. Lensen A, van Druten J, Bolmer M, van Gemert J, Eling W, Sauerwein RW, 1996. Measurement by membrane feeding of reduction in Plasmodium falciparum transmission induced by endemic area. Trans R Soc Trop Med Hyg 90 : 20–22. [Google Scholar]
  16. Carter R, Nijhout MM, 1977. Control of gamete formation (ex-flagellation) in malaria parasites. Science 195 : 407–409. [Google Scholar]
  17. Arai M, Billker O, Morris HR, Panico M, Delcroix M, Dixon D, Ley SV, Sinden RE, 2001. Both mosquito-derived xanthurenic acid and a host blood-derived factor regulate gametogenesis of Plasmodium in the midgut of the mosquito. Mol Bioch Para-sitol 116 : 17–24. [Google Scholar]
  18. Nijhout MM, Carter R, 1978. Gamete development in malaria parasites: bicarbonate-dependent stimulation by pH in vitro. Parasitol 76 : 39–53. [Google Scholar]
  19. Moorthy VS, Good MF, Hill AVS, 2004. Malaria vaccine developments. Lancet 363 : 150–156. [Google Scholar]
  20. Clapham DE, 1995. Calcium signaling. Cell 80 : 259–268. [Google Scholar]
  21. Doerig CD, 1997. Signal transduction in malaria parasites. Para-sitol Today 13 : 307–313. [Google Scholar]
  22. Muhia DK, Swales CA, Deng W, Kelly JM, Baker DA, 2001. The gametocyte-activating factor xanthurenic acid stimulates an increase in membrane-associated guanylyl cyclase activity in the human malaria parasite Plasmodium falciparum. Mol Microbiol 42 : 553–560. [Google Scholar]
  23. Billker O, Tewari R, Franke-Fayard B, Brinkman V, 2004. Calcium and a calcium-dependent protein kinase regulate gamete formation and mosquito transmission in a malaria parasite. Cell 117 : 503–514. [Google Scholar]
  24. Li JL, Baker DA, Cox LS, 2000. Sexual stage-specific expression of a third calcium-dependent protein kinase from Plasmodium falciparum. Biochim Biophys Acta 1491 : 341–349. [Google Scholar]
  25. Rawlings DJ, Kaslow DC, 1992. A novel 40-kDa membrane-associated EF-hand calcium-binding protein in Plasmodium falciparum. J Biol Chem 267 : 3976–3982. [Google Scholar]

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  • Received : 26 Dec 2006
  • Accepted : 01 Apr 2007

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