Volume 77, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


culture-derived, soluble, exogenous antigens have been shown to be a source of vaccine targets for the parasite. We have previously reported that culture-derived, soluble, exogenous antigens can immunize BALB/c mice against challenge with . However, the molecule(s) involved in this protection was not known. We describe the potential of one component of soluble exogenous antigens (recombinant nucleoside hydrolase) to vaccinate mice against challenge with . We found that recombinant nucleoside hydrolase vaccinated BALB/c mice against a subsequent challenge with . Protection was manifested by a significant decrease in lesion size (as much as a 30-fold reduction) and parasite burden (as much as a 71-fold reduction). Protection was achieved whether recombinant nucleoside hydrolase was administered to mice in the presence or absence of adjuvant (interleukin-12). Finally, protection was accompanied by an increase in interferon-γ production but a decrease in interleukin-10 production by vaccinated animals in response to challenge with .


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  • Received : 26 Jun 2007
  • Accepted : 22 Aug 2007

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