1921
Volume 77, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

To better understand the factors involved in maternal–fetal transmission of , we compared DNA levels—obtained by use of quantitative real-time PCR and parasitic genotypes determined by PCR amplification followed by hybridization—in Bolivian mothers and their congenitally infected newborns. Mothers and their neonates displayed markedly different parasitic DNA levels, as most maternal estimated parasitemias (> 90%) were < 10 parasites/mL, whereas those of 76% of their newborns were > 1,000 parasites/mL. Comparison of sublineages infecting mothers and newborns showed identity, without evidence of mixed infection in mothers or neonates. Analysis of minor variants of -genotyped parasites using sequence class probes hybridizing with hypervariable domains of kDNA minicircles showed discrepancies in half of mother/newborn pairs.

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2007-07-01
2017-09-20
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  • Received : 21 Feb 2007
  • Accepted : 18 Apr 2007

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