1921
Volume 76, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

infects nearly 150 million people, primarily in Africa, and is transmitted by select species of local bulinid snails. These snails can host other related trematode species as well, so that effective detection and monitoring of snails infected with requires a successful differentiation between and any closely related schistosome species. To enable differential detection of DNA by simple polymerase chain reaction (PCR), we designed and tested primer pairs from numerous newly identified DNA repeat sequences. However, all pairs tested were found unsuitable for this purpose. Differentiation of from and (but not from ) was ultimately accomplished by PCR using one primer from a newly identified repeat, Sh110, and a second primer from a known schistosomal splice-leader sequence. For evaluation of residual transmission after control interventions, this differentiation tool will enable accurate monitoring of infected snails in areas where is sympatric with the most prevalent other schistosome species.

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References

  1. WHO, 2002. Prevention and control of schistosomiasis and soil-transmitted helminthiasis: report of a WHO expert committee. World Health Organ Tech Rep Ser 912 : 2–3. [Google Scholar]
  2. Loker ES, 1983. A comparative study of the life-histories of mammalian schistosomes. Parasitology 87 : 343–369. [Google Scholar]
  3. Rollinson D, Stothard JR, Southgate VR, 2001. Interactions between intermediate snail hosts of the genus Bulinus and schistosomes of the Schistosoma haematobium group. Parasitology 123 (Suppl): S245–S260. [Google Scholar]
  4. Chipaux JP, Boulanger D, Bremond P, Campagne G, Vera C, Sellin B, 1997. The WHO Collaborating Centre for Research and Control of schistosomiasis at Niamey, Niger. Mem Inst Oswaldo Cruz 92 : 725–728. [Google Scholar]
  5. Woolhouse ME, Chandiwana SK, 1989. Spatial and temporal heterogeneity in the population dynamics of Bulinus globosus and Biomphalaria pfeifferi and in the epidemiology of their infection with schistosomes. Parasitology 98 : 21–34. [Google Scholar]
  6. Woolhouse MEJ, Chandiwana SK, 1990. The epidemiology of schistosome infections of snails: taking the theory into the field. Parasitol Today 6 : 65–70. [Google Scholar]
  7. Christensen NO, Mutani A, Frandsen F, 1983. A review of the biology and transmission ecology of African bovine species of the genus Schistosoma. Z Parasitenkd 69 : 551–570. [Google Scholar]
  8. Mone H, Mowahid G, Monard S, 2000. The distribution of Schistosoma bovis Sonsino, 1876 in relation to intermediate host mollusc-parasite relationship. Adv Parasitol 44 : 100–138. [Google Scholar]
  9. Jourdane J, Southgate VR, Pages JR, Durand P, Tchuente LAT, 2001. Recent studies on Schistosoma intercalatum: taxonomic status, puzzling distribution, and transmission foci revisited. Mem Inst Oswaldo Cruz 96 : 45–48. [Google Scholar]
  10. Barber KE, Mkoji M, Loker ES, 2000. PCR-RFLP analysis of the ITS2 region to identify Schistosoma haematobium and S. bovis from Kenya. Am J Trop Med Hyg 62 : 434–440. [Google Scholar]
  11. Ross GC, Southgate VR, Knowles RJ, 1978. Observations on some isozymes of strains of Schistosoma bovis, S. mattheei, S. margrebowiei and S. leiperi. Z Parasitenkd 57 : 49–56. [Google Scholar]
  12. Walker TK, Rollinson D, Simpson AJG, 1986. Differentiation of Schistosoma haematobium from related species using cloned ribosomal RNA gene probes. Mol Biochem Parasitol 21 : 123–131. [Google Scholar]
  13. Dias Neto E, Pereira de Souza C, Rollinson D, Katz N, Pena SDJ, Sympson AJG, 1993. The random amplification of Polymorphic DNA allows the identification of strains and species of schistosome. Mol Biochem Parasitol 57 : 83–88. [Google Scholar]
  14. Hamburger J, He N, Abbasi I, Ramzy RM, Jourdane J, Ruppel A, 2001. Polymerase chain reaction assay based on a highly repeated sequence of Schistosoma haematobium: a potential tool for monitoring schistosome-infested water. Am J Trop Med Hyg 65 : 907–911. [Google Scholar]
  15. Hamburger J, Hoffman O, Kariuki HC, Muchiri EM, Ouma JH, Koech DK, Sturrock RF, King CH, 2004. Large-scale, polymerase chain reaction-based surveillance of Schistosoma haematobium DNA in snails from transmission sites in coastal Kenya: a new tool for studying the dynamics of snail infection. Am J Trop Med Hyg 71 : 765–773. [Google Scholar]
  16. Kariuki HC, Clennon JA, Brady M, Kitron U, Sturrock RF, Ouma JH, Tosha S, Ndzovhu M, Mungai P, Hamburger J, Hoffman O, Pellegrini C, Muchiri EM, King CH, 2004. Distribution patterns and cercarial shedding of Bulinus nasutus and other snails in the Msambweni area, Coast Province, Kenya. Am J Trop Med Hyg 70 : 449–456. [Google Scholar]
  17. Sambrook J, Fritsch EF, Maniatis, 1989. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
  18. Buluwela L, Forster A, Boehm T, Rabbitts TH, 1989. A rapid procedure for colony screening using nylon filters. Nucleic Acids Res 17 : 452. [Google Scholar]
  19. Rajkovic A, Davis RS, Simonsen N, Rottman F, 1990. A splice leader is present on a subset of mRNAs from the human parasite Schistosoma mansoni. Proc Natl Acad Sci USA 87 : 8879–8883. [Google Scholar]
  20. Hamburger J, Turetski T, Kapeller I, Deresievicz R, 1991. Highly repeated short DNA sequences in the genome of Schistosoma mansoni recognized by a species-specific probe. Mol Biochem Parasitol 44 : 73–80. [Google Scholar]
  21. Abbasi I, Branzburg A, Campos-Ponce M, Abdel Hafez SK, Raoul F, Craig P, Hamburger J, 2003. Coprodiagnosis of Echinococcus granulosus infection in dogs by amplification of a newly identified repeated sequence. Am J Trop Med Hyg 69 : 324–330. [Google Scholar]
  22. Simpson AJ, Sher A, McCutchan TF, 1982. The genome of Schistosoma mansoni: Isolation of DNA, its size, bases and repetitive sequences. Mol Biochem Parasitol 6 : 125–137. [Google Scholar]
  23. Vercruysse J, Southgate VR, Rollinson D, DeClercq D, Sacko M, De Bont J, Mungomba LM, 1994. Studies on transmission and schistosome interactions in Senegal, Mali, and Zambia. Trop Geogr Med 46 : 220–226. [Google Scholar]
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  • Received : 10 Sep 2006
  • Accepted : 27 Oct 2006

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