Volume 76, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


infects nearly 150 million people, primarily in Africa, and is transmitted by select species of local bulinid snails. These snails can host other related trematode species as well, so that effective detection and monitoring of snails infected with requires a successful differentiation between and any closely related schistosome species. To enable differential detection of DNA by simple polymerase chain reaction (PCR), we designed and tested primer pairs from numerous newly identified DNA repeat sequences. However, all pairs tested were found unsuitable for this purpose. Differentiation of from and (but not from ) was ultimately accomplished by PCR using one primer from a newly identified repeat, Sh110, and a second primer from a known schistosomal splice-leader sequence. For evaluation of residual transmission after control interventions, this differentiation tool will enable accurate monitoring of infected snails in areas where is sympatric with the most prevalent other schistosome species.


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  • Received : 10 Sep 2006
  • Accepted : 27 Oct 2006

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