1921
Volume 76, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

A multiplex PCR assay has been developed for detection of cryptic species, their human host preference, and presence in the mosquito. PCR conditions were optimized using primer sets specific for cryptic species, and and evaluated with field-collected mosquitoes. A unique mosquito processing method was used for screening carrying capacity and human host preference of mosquitoes in first-round multiplex PCR. The vectorial status of the mosquito for parasite was confirmed in second-round PCR. Of the 121 collected mosquitoes, 92 were of S type, 26 of T type, and 3 were of other types. Human host preference was dominant in S type, of which 4% were sporozoite positive. This assay and processing method can also be used to evaluate vector competence of other anophelines.

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2007-05-01
2017-11-24
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References

  1. World Health Organization, 2003. The World Health Report 2003: Shaping the Future. Geneva: World Health Organization.
  2. World Health Organization andUnited Nations Children’s Fund, 2005. World Malaria Report 2005. Geneva: World Health Organization and UNICEF.
  3. Fontenille D, Louchouarn L, 1999. The complexity of the malaria vectorial system in Africa. Parassitologia 41 : 267–271.
  4. Subbarao SK, 1998. Anopheles Species Complexes in South East Asia Region. Technical Publication No. 18. New Delhi: World Health Organization, South East Asia Regional Office.
  5. Beebe NW, Saul A, 1995. Discrimination of all members of the Anopheles punctulatus complex by polymerase chain reaction-restriction fragment length polymorphism analysis. Am J Trop Med Hyg 53 : 478–481.
  6. Cornel AJ, Porter CH, Collins FH, 1996. PCR diagnostic assay for Anopheles quadrimaculatus cryptic species (Diptera: Culicidae) based on ribosomal DNA ITS2 sequences. J Med Entomol 33 : 109–116.
  7. Kampen H, Sternberg A, Proft J, Bastian S, Schaffner F, Maier WA, Seitz HM, 2003. Polymerase chain reaction-based differentiation of the mosquito sibling species Anopheles claviger s.s. and Anopheles petragnani (Diptera: Culicidae). Am J Trop Med Hyg 69 : 195–199.
  8. Proft J, Maier WA, Kampen H, 1999. Identification of six sibling species of the Anopheles maculipennis complex (Diptera: Culicidae) by a polymerase chain reaction assay. Parasitol Res 85 : 837–843.
  9. Sharpe RG, Hims MM, Harbach RE, Butlin RK, 1999. PCR based methods for identification of species of the Anopheles minimus group: allele-specific amplification and single-strand conformation polymorphism. Med Vet Entomol 13 : 265–273.
  10. Reimer LJ, Tripet F, Slotman M, Spielman A, Fondjo E, Lanzaro GC, 2005. An unusual distribution of the kdr gene among populations of Anopheles gambiae on the island of Bioko, Equatorial Guinea. Insect Mol Biol 14 : 683–688.
  11. Surendran SN, Ramasamy MS, De Silva BG, Ramasamy R, 2006. Anopheles culicifacies cryptic species B and E in Sri Lanka differ in longevity and in their susceptibility to malaria parasite infection and common insecticides. Med Vet Entomol 20 : 153–156.
  12. Sharma VP, 1998. Fighting malaria in India. Curr Sci 75 : 1127–1140.
  13. Rao TR, 1984. The Anophelines of India. New Delhi: Malaria Research Centre, Indian Council of Medical Research.
  14. Subbarao SK, Nanda N, Vasantha K, Dua VK, Malhotra MS, Yadav RS, Sharma VP, 1994. Cytogenetic evidence for three sibling species in Anopheles fluviatilis (Diptera: Culicidae). Ann Entomol Soc Am 87 : 116–121.
  15. Nanda N, Joshi H, Subbarao SK, Yadav RS, Shukla RP, Dua VK, Sharma VP, 1996. Anopheles fluviatilis complex: host feeding pattern of species S, T and U. J Am Mosq Control Assoc 12 : 147–149.
  16. Nanda N, Yadav RS, Subbarao SK, Joshi H, Sharma VP, 2000. Studies on Anopheles fluviatilis and Anopheles culicifacies sibling species in relation with malaria in forested hilly and deforested riverine eco-systems in northern Orissa in India. J Am Mosq Control Assoc 16 : 199–205.
  17. Sharma SK, Nanda N, Dua VK, Joshi H, Subbarao SK, Sharma VP, 1995. Studies on the bionomics of Anopheles fluviatilis sensu lato and sibling species composition in the foothills of Shivalik range (Uttar Pradesh), India. Southeast Asian J Trop Med Public Health 26 : 566–572.
  18. Manonmani A, Townson H, Adeniran T, Jambulingam P, Sahu S, Vijayakumar T, 2001. rDNA-ITS2 polymerase chain reaction assay for the sibling species of Anopheles fluviatilis. Acta Trop 78 : 3–9.
  19. Singh OP, Chandra D, Nanda N, Raghavendra K, Sunil S, Sharma SK, Dua VK, Subbarao SK, 2004. Differentiation of members of the Anopheles fluviatilis species Complex by an allele-specific polymerase chain reaction based on 28s ribosomal dna sequences. Am J Trop Med Hyg 70 : 27–32.
  20. Edrissian GH, Manouchehry AV, Hafizi A, 1985. Application of an enzyme-linked immunosorbent assay (ELISA) for determination of the human blood index in anopheline mosquitoes collected in Iran. J Am Mosq Control Assoc 1 : 349–352.
  21. Savage HM, Duncan JF, Roberts DR, Sholdt LL, 1991. A dipstick ELISA for rapid detection of human blood meals in mosquitoes. J Am Mosq Control Assoc 7 : 16–23.
  22. Thapar BR, Sharma SN, Dasgupta RK, Kaul SM, Bali A, Chhabra K, Lal S, 1998. Blood meal identification by using Microdot ELISA in vector mosquitoes. J Commun Dis 30 : 283–287.
  23. Collins RT, Narasimham MV, Dhal KB, Mukherjee BP, 1991. Gel diffusion analysis of Anopheles bloodmeals from 12 malarious study villages of Orissa State, India. J Am Mosq Control Assoc 7 : 595–603.
  24. Kent RJ, Norris DE, 2005. Identification of mammalian blood meals in mosquitoes by a multiplexed polymerase chain reaction targeting cytochrome B. Am J Trop Med Hyg 73 : 336–342.
  25. Oshaghi MA, Chavshin AR, Vatandoost H, Yaaghoobi F, Mohtarami F, Noorjah N, 2006. Effects of post-ingestion and physical conditions on PCR amplification of host blood meal DNA in mosquitoes. Exp Parasitol 12 : 232–236.
  26. Appawu MA, Bosompem KM, Dadzie S, McKakpo US, Anim-Baidoo I, Dykstra E, Szumlas DE, Rogers WO, Koram K, Fryauff DJ, 2003. Detection of malaria sporozoites by standard ELISA and VecTest™ dipstick assay in field-collected anopheline mosquitoes from a malaria endemic site in Ghana. Trop Med Int Health 8 : 1012–1017.
  27. Arez AP, Lopes D, Pinto J, Franco AS, Snounou G, do Rosario VE, 2000. Plasmodium sp.: optimal protocols for PCR detection of low parasite numbers from mosquito (Anopheles sp.) samples. Exp Parasitol 94 : 269–272.
  28. Moreno M, Cano J, Nzambo S, Bobuakasi L, Buatiche JN, Ondo M, Micha F, Benito A, 2004. Malaria Panel Assay versus PCR: detection of naturally infected Anopheles melas in a coastal village of Equatorial Guinea. Malar J 3 : 20.
  29. Tassanakajon A, Boonsaeng V, Wilairat P, Panyim S, 1993. Polymerase chain reaction detection of Plasmodium falciparum in mosquitoes. Trans R Soc Trop Med Hyg 87 : 273–275.
  30. Wilson MD, Ofosu-Okyere A, Okoli AU, McCall PJ, Snounou G, 1998. Direct comparison of microscopy and polymerase chain reaction for the detection of Plasmodium sporozoites in salivary glands of mosquitoes. Trans R Soc Trop Med Hyg 92 : 482–483.
  31. Orlandi PA, Lampel KA, 2000. Extraction-free, filter-based template preparation for rapid and sensitive PCR detection of pathogenic parasitic protozoa. J Clin Microbiol 38 : 2271–2277.
  32. Christophers SR, 1933. The Fauna of British India. Dipter, Vol IV. Family-Culicidae. Tribe-Anophelini. New Delhi: Today and Tomorrow’s Printers and Publishers.
  33. Nagpal BN, Srivastava A, Saxena R, Ansari MA, Dash AP, Das SC, 2005. Pictorial Identification Key for Indian Anophelines. New Delhi: Malaria Research Centre (ICMR).
  34. Casimiro S, Coleman M, Mohloai P, Hemingway J, Sharp B, 2006. Insecticide resistance in Anopheles funestus (Diptera: Culicidae) from Mozambique. J Med Entomol 43 : 267–275.
  35. Casimiro S, Coleman M, Hemingway J, Sharp B, 2006. Insecticide resistance in Anopheles arabiensis and Anopheles gambiae from Mozambique. J Med Entomol 43 : 276–282.
  36. Kelly-Hope LA, Yapabandara AM, Wickramasinghe MB, Perera MD, Karunaratne SH, Fernando WP, Abeyasinghe RR, Siyambalagoda RR, Herath PR, Galappaththy GN, Hemingway J, 2005. Spatiotemporal distribution of insecticide resistance in Anopheles culicifacies and Anopheles subpictus in Sri Lanka. Trans R Soc Trop Med Hyg 99 : 751–761.
  37. Overgaard HJ, Sandve SR, Suwonkerd W, 2005. Evidence of anopheline mosquito resistance to agrochemicals in northern Thailand. Southeast Asian J Trop Med Public Health 36 (Suppl 4): 152–157.
  38. Collins FH, Paskewitz SM, 1996. A review of the use of ribosomal DNA to differentiate among cryptic Anopheles species. Insect Mol Biol 5 : 1–9.
  39. World Health Organization, 1975. Manual on practical entomology in malaria. Part II. Offset Publication 13. Geneva: World Health Organization (WHO).
  40. Wirtz RA, Burkot TR, Andre RG, Rosenberg R, Collins WE, Roberts DR, 1985. Identification of Plasmodium vivax sporozoites in mosquitoes using an enzyme-linked immunosorbent assay. Am J Trop Med Hyg 34 : 1048–1054.
  41. Wirtz RA, Sattabongkot J, Hall T, Burkot TR, Rosenberg R, 1992. Development and evaluation of an ELISA for Plasmodium vivax-VK247 sporozoites. J Med Entomol 29 : 854–857.
  42. Fucharoen D, Tirawanchai N, Wilairat P, Panyim S, Thaithong S, 1988. Differentiation of Plasmodium falciparum clones by means of a repetitive DNA probe. Trans R Soc Trop Med Hyg 82 : 209–211.
  43. Ryan JR, Dave K, Collins KM, Hochberg L, Sattabongkot J, Coleman RE, Dunton RF, Bangs MJ, Mbogo CM, Cooper RD, Schoeler GB, Rubio-Palis Y, Magris M, Romero LI, Padilla N, Quakyi IA, Bigoga J, Leke RG, Akinpelu O, Evans B, Walsey M, Patterson P, Wirtz RA, Chan AST, 2002. Extensive multiple test centre evaluation of the VecTest™ malaria antigen assay. Med Vet Entomol 16 : 321–327.
  44. Kabiru EW, Mbogo CM, Muiruri SK, Ouma JH, Githure JI, Beier JC, 1997. Sporozoite loads of naturally infected Anopheles in Kilifi District, Kenya. J Am Mosq Control Assoc 13 : 259–262.
  45. Beier JC, Davis JR, Vaughan JA, Noden BH, Beier MS, 1991. Quantitation of Plasmodium falciparum sporozoites transmitted in vitro by experimentally infected Anopheles gambiae and Anopheles stephensi. Am J Trop Med Hyg 44 : 564–570.
  46. Cano J, Berzosa PJ, Roche J, Rubio JM, Moyano E, Guerra-Neira A, Brochero H, Mico M, Edu M, Benito A, 2004. Malaria vectors in Bioko Island (Equatorial Guinea): estimation of vector dynamics and transmission intensities. J Med Entomol 41 : 158–162.
  47. Kain KC, Wirtz RA, Fernandez I, Franke ED, Rodriguez MH, Lanar DE, 1992. Serological and genetic characterization of Plasmodium vivax from whole blood-impregnated filter paper discs. Am J Trop Med Hyg 46 : 473–479.
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  • Received : 07 Aug 2006
  • Accepted : 13 Dec 2006

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