Volume 76, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


A multiplex PCR assay has been developed for detection of cryptic species, their human host preference, and presence in the mosquito. PCR conditions were optimized using primer sets specific for cryptic species, and and evaluated with field-collected mosquitoes. A unique mosquito processing method was used for screening carrying capacity and human host preference of mosquitoes in first-round multiplex PCR. The vectorial status of the mosquito for parasite was confirmed in second-round PCR. Of the 121 collected mosquitoes, 92 were of S type, 26 of T type, and 3 were of other types. Human host preference was dominant in S type, of which 4% were sporozoite positive. This assay and processing method can also be used to evaluate vector competence of other anophelines.


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  • Received : 07 Aug 2006
  • Accepted : 13 Dec 2006

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