Volume 76, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Polymerase chain reaction (PCR) was applied to confirm the diagnosis of brucellosis and to study its clearance in response to the standard treatment regimen with doxycycline and rifampin at hospitals in Callao and Lima, Peru. The PCR confirmed the diagnosis in 23 (91.7%) patients with brucellosis including 12 culture-confirmed cases. For patients treated at the hospital in Callao, PCR was positive for all samples collected during and at the conclusion of treatment and for 76.9% of follow-up samples collected on average 15.9 weeks after completion of treatment. For patients treated at the hospital in Lima, PCR tests were positive for 81.8% of samples collected during treatment, for 33.3% of samples collected at the conclusion of treatment, and for ≥ 50% of samples collected at first, second, and third post-treatment follow-up. Thus, DNA may persist in the serum weeks to months after completion of the standard treatment regimen.


Article metrics loading...

Loading full text...

Full text loading...



  1. Refai M, 2002. Incidence and control of brucellosis in the Near East region. Vet Microbiol 20 : 81–110.
  2. Young EJ, 1995a. An overview of human brucellosis. Clin Infect Dis 21 : 283–289.
  3. Morgan WTB, McDiarmid A, 1960. The excretion of Brucella abortus in the milk of experimentally infected cattle. Res Vet Sci 1 : 53–56.
  4. Nicoletti P, 1980. The epidemiology of bovine brucellosis. Adv Vet Sci Comp Med 24 : 69–84.
  5. Guillen A, Navarro AM, Acosta M, Arrelucé M, 1989. Brucellosis epidemiology. In: Annals of the National Seminary of Zoonosis and Diseases of Nourishing Transmission. Lima: Ministry of Health; 48–65.
  6. Young EJ, 1995b. Brucellosis: Current epidemiology, diagnosis, and management. Curr Clin Top Infect Dis 15 : 115–128.
  7. Solera J, Rodriguez-Zapata M, Geijo P, Largo J, Paulino J, Saez L, Martinez-Alfaro E, Sanchez L, Sepulveda MA, Ruiz-Ribo MD, 1995. Doxycycline-rifampin versus doxycyclin-streptomycin in treatment of human brucellosis due to Brucella melitensis. Antimicrob Agents Chemother 39 : 2061–2067.
  8. Ariza J, Gudiol F, Pallares R, Viladrich PF, Rufi G, Corredoira J, Miravitlles MR, 1992. Treatment of human brucellosis with doxycycline plus rifampin or doxycycline plus streptomycin. Ann Intern Med 117 : 25–30.
  9. Ersoy Y, Sonmez E, Tevfik MR, But AD, 2005. Comparison of three different combination therapies in the treatment of human brucellosis. Trop Doct 35 : 210–212.
  10. Pappas G, Solera J, Akritidis N, Tsianos E, 2005. New approaches to the antibiotic treatment of brucellosis. Int J Antimicrob Agents 26 : 101–105.
  11. Yagupsky P, 1999. Detection of Brucellae in blood cultures. J Clin Microbiol 37 : 3437–3442.
  12. Al Dahouk S, Tomaso H, Nockler K, Neubauer H, Franoulidis D, 2003. Laboratory- based diagnosis of brucellosis—a review of the literature. Part II: Serological tests for brucellosis. Clin Lab 49 : 577–589.
  13. Navarro E, Segura JC, Castano MJ, Solera J, 2006. Use of real-time quantitative polymerase chian reaction to monitor the evoluation of Brucella melitentis DNA load during therapy and post-treatment follow-up in patients with brucellosis. Clin Inf Dis. 42 : 1266–1273.
  14. Baily GG, Krahn JB, Drasar BS, Stoker NG, 1992. Detection of Brucella melitensis and Brucella abortus by DNA amplification. J Trop Med Hyg 95 : 271–275.
  15. Alton GG, Jones LM, Pietz DF, 1975. WHO Laboratory Techniques in Brucellosis. Geneva: World Health Organization.
  16. Smits HL, Abdoel TH, Solera J, Clavijo E, Diaz R, 2003. Immunochromatographic Brucella-specific immunoglobulin M and G lateral flow assays for the serodiagnosis of human brucellosis. Clin Diagn Lab Immunol 10 : 1141–1146.
  17. Navarro E, Casao MA, Solera J, 2004. Diagnosis of human brucellosis using PCR. Expert Rev Mol Diagn 4 : 115–123.
  18. Queipo-Ortuno MI, Morata P, Ocon P, Manchado P, Dios Colmenero J, 1997. Rapid diagnosis of human brucellosis by peripheral-blood PCR assay. J Clin Microbiol 35 : 2927–2930.
  19. Matar GM, Khneisser IA, Abdelnoor AM, 1996. Rapid laboratory confirmation of human brucellosis by PCR analysis of a target sequence on the 31-kilodalton Brucella antigen DNA. J Clin Microbiol 34 : 477–478.
  20. Morata P, Queipo-Ortuno MI, Reguera JM, Miralles F, Lopez-Gonzalez JJ, Colmenero JD, 2001. Diagnostic yield of a PCR assay in focal complications of brucellosis. J Clin Microbiol 39 : 3743–3746.
  21. Zerva L, Bourantas K, Mitka S, Kansouzidou A, Legakis NJ, 2001. Serum is the preferred clinical specimen for diagnosis of human brucellosis by PCR. J Clin Microbiol 39 : 1661–1664.
  22. Ariza J, Gudiol F, Pallares R, Rufi G, Fernandez-Viladrich P, 1985. Comparative trial of rifampin-doxycycline versus tetracycline-streptomycin in the therapy of human brucellosis. Antimicrob Agents Chemother 28 : 548–551.
  23. Irmak H, Buzgan T, Evirgen O, Akdeniz H, Pekcan Demiroz A, Abdoel TH, Smits HL, 2004. Use of the Brucella IgM and IgG flow assays in the serodiagnosis of human brucellosis in an area endemic for brucellosis. Am J Trop Med Hyg 70 : 688–694.
  24. Morata P, Queipo-Ortuno MI, Reguera JM, Garcia-Ordonez MA, Pichardo C, Colmenero JD, 1999. Posttreatment follow-up of brucellosis by PCR assay. J Clin Microbiol 37 : 4163–4166.
  25. Elfaki MG, Al-Hokail AA, Nakeeb SM, Al-Rabiah FA, 2005. Evaluation of culture, tube agglutination, and PCR methods for the diagnosis of brucellosis in humans. Med Sci Monit 11 : 69–74.
  26. Buchanan TM, Faber LC, 1980. 2-mercaptoethanol Brucella agglutination test: Usefulness for predicting recovery from brucellosis. J Clin Microbiol 11 : 691–693.
  27. Hasanjani Roushan MR, Mohraz M, Hajiahmadi M, Ramzani A, Valayati AA, 2006. Efficacy of gentamicin plus doxycycline versus streptomycin plus doxycycline in the treatment of brucellosis in humans. Clin Infect Dis 42 : 1075–1080.
  28. Falagas ME, Bliziotis IA, 2006. Quinolones for treatment of human brucellosis: critical review of the evidence from microbiological and clinical studies. Antimicrob Agents Chemother 50 : 22–33.

Data & Media loading...

  • Received : 29 Aug 2006
  • Accepted : 01 Nov 2006

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error