Volume 76, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


We studied the tick, to determine the possibility of both transovarial and transstadial transmission of . We also studied the usefulness of the needle injection method for pathogenic tick-transmitted organisms including parasites. Erythrocytes infected with were injected into the midgut of engorged adults or nymphs using a hypodermic needle passed through the integument. DNA of in ticks was detected using nested polymerase chain reaction (PCR). DNA was present in adults, eggs, and larvae, indicating that transovarial transmission occurred. DNA was present in adults that developed from infected nymphs, and the antigen was present in their salivary glands, indicating that transstadial transmission occurred. These findings suggest that may play a role in the transmission of .


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  • Received : 29 Mar 2006
  • Accepted : 18 Aug 2006

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