Volume 76, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


The requirements for effective and efficient intracellular killing of by activated macrophages are unknown. Despite resistance to the arginase inhibitor LOHA by intracellular amastigotes, enhanced replication did not account for the relative resistance of this parasite to macrophage activation. Herein we report that the presence of both superoxide and nitric oxide is necessary for efficient killing of amastigotes within LPS/IFN-γ–activated bone marrow-derived macrophages generated from C3H mice. Addition of an extracellular signal-regulated kinase (ERK) inhibitor to infected macrophages increased the ability of these activated macrophages to kill amastigotes. This enhanced macrophage killing through addition of ERK inhibitor was abrogated by inhibition of superoxide or iNOS, whereas inhibiting superoxide had no effect on the killing of . These results suggest that ERK activation may modulate effective macrophage killing, leading to the ability of to resist elimination within activated macrophages.


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  • Received : 14 Jul 2005
  • Accepted : 08 Dec 2006

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