1921
Volume 76, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

The principal agent of mucocutaneous leishmaniasis (MCL) is the South American protozoan parasite () . This organism is generally considered to be clonal, that is, it does not to undergo genetic exchange. Nevertheless, apparent hybrids between several species have been reported in the New World and the Old World. When we characterized isolates of () from a single focus of cutaneous leishmaniasis (CL) and MCL, we found a remarkable phenotypic and genotypic diversity, with 12 zymodemes and 20 microsatellite genotypes. Furthermore, 26 of the 59 isolates were / phenotypic hybrids that displayed 7 different microsatellite genotypes. A hybrid genotype was the only organism isolated from 4 patients with MCL. Thus hybrids must be included among the potential agents of MCL. Despite the propensity for clonality, hybrids are also an important feature of () and may give rise to epidemiologically important emergent genotypes.

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2007-03-01
2017-11-18
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References

  1. Lucas CM, Franke ED, Cachay MI, Tejada A, Cruz ME, Kreutzer RD, Barrer DC, McCann SH, Watts DM, 1998. Geographic distribution and clinical description of leishmaniasis cases in Peru. Am J Trop Med Hyg 59 : 312–317.
  2. Guthman JP, Arlt D, Garcia LM, Rosales M, de Jesus Sanchez J, Alvarez E, Lonlas S, Conte M, Bertoletti G, Fournier C, Huari R, Torreele E, Llanos-Cuentas A, 2005. Control of mucocutaneous leishmaniasis, a neglected disease: results of a control programme in Satipo Province, Peru. Trop Med Int Health 10 : 856–862.
  3. Reithinger R, Espinoza JC, Llanos-Cuentas A, Davies CR, 2003. Domestic dog ownership: a risk factor for human infection with Leishmania (Viannia) species. Trans R Soc Trop Med Hyg 97 : 141–145.
  4. Llanos-Cuentas EA, Roncal N, Villaseca P, Paz L, Ogusuku E, Perez JE, Caceres A, Davies CR, 1999. Natural infections of Leishmania peruviana in animals in the Peruvian Andes. Trans R Soc Trop Med Hyg 93 : 15–20.
  5. Brandao-Filho SP, Brito ME, Carvalho FG, Ishikawa EA, Cupolillo E, Floeter-Winter L, Shaw JJ, 2003. Wild and synanthropic hosts of Leishmania (Viannia) braziliensis in the endemic cutaneous leishmaniasis locality of Amaraji, Pernambuco State, Brazil. Trans R Soc Trop Med Hyg 97 : 291–296.
  6. Dujardin JC, Banuls AL, Llanos-Cuentas A, Alvarez E, De-Doncker S, Jacquet D, Le Ray D, Arevalo J, Tibayrenc M, 1995. Putative Leishmania hybrids in the Eastern Andean valley of Huanuco, Peru. Acta Trop 59 : 293–307.
  7. Grimaldi G Jr, Momen H, Naiff RD, McMahon-Pratt D, Barrett TV, 1991. Characterization and classification of leishmanial parasites from humans, wild mammals, and sand flies in the Amazon region of Brazil. Am J Trop Med Hyg 44 : 645–661.
  8. Evans DA, Godfrey D, Lanham S, Lanotte G, Modabber F, Schnur L, 1999. Handbook on Isolation, Characterization and Cryopreservation of Leishmania. UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR). Geneva, Switzerland: WHO.
  9. Belli AA, Miles MA, Kelly JM, 1994. A putative Leishmania panamensis/Leishmania braziliensis hybrid is a causative agent of human cutaneous leishmaniasis in Nicaragua. Parasitology 109 : 435–442.
  10. Ostenreither S, Kuhls K, Schaar M, Presber W, Schönian G, 2006. Multilocus microsatellite typing as a new tool for discrimination of Leishmania infantum MON-1 strains. J Clin Microbiol 44 : 495–503.
  11. Russell R, Iribar MP, Lambson B, Brewster S, Blackwell JM, Dye C, Ajioka JW, 1999. Intra and inter-specific microsatellite variation in the Leishmania subgenus Viannia. Mol Biochem Parasitol 103 : 71–77.
  12. Hartl DL, Clark AG, 1997. Principles of Population Genetics. Sunderland, MA: Sinauer Associates Inc.
  13. Hedrick PW, 2004. Genetics of Populations. Third edition. Boston, MA: Jones and Bartlett Publishers Inc.
  14. Maynard-Smith J, Smith NH, O’Rourke M, Spratt BG, 1993. How clonal are bacteria? Proc Natl Acad Sci USA 90 : 4384–4388.
  15. MacLeod A, Turner CM, Tait A, 2001. The detection of geographical substructuring of Trypanosoma brucei populations by the analysis of minisatellite polymorphisms. Parasitology 123 : 475–482.
  16. Gibson W, Whittington H, 1993. Genetic exchange in Trypanosoma brucei: selection of hybrid trypanosomes by introduction of genes conferring drug resistance. Mol Biochem Parasitol 60 : 19–26.
  17. MacLeod A, Tweedie A, McLellan S, Taylor T, Cooper C, Sweeney L, Turner CMR, Tait A, 2005. Allelic segregation and independent assortment in T. brucei crosses: Proof that the genetic system is Mendelian and involves meiosis. Mol Biochem Parasitol 143 : 12–19.
  18. Machado CA, Ayala FJ, 2001. Nucleotide sequences provide evidence of genetic exchange among distantly related lineages of Trypanosoma cruzi. Proc Natl Acad Sci USA 98 : 7396–7401.
  19. Gaunt MW, Yeo M, Frame IA, Stothard JR, Carrasco HJ, Taylor MC, Mena SS, Veazey P, Miles GA, Acosta N, de Arias AR, Miles MA, 2003. Mechanism of genetic exchange in American trypanosomes. Nature 421 : 936–939.
  20. Heitman J, 2006. Sexual reproduction and the evolution of microbial pathogens. Curr Biol 16 : R711–R725.
  21. Bennett RJ, Johnson AD, 2003. Completion of a parasexual cycle in Candida albicans by induced chromosome loss in tetraploid strains. EMBO J 22 : 2505–2515.
  22. Delgado O, Cupolillo E, Bonfante Garrido R, Silva S, Belfort E, Grimaldi Junior G, Momen H, 1997. Cutaneous leishmaniasis in Venezuela caused by infection with a new hybrid between Leishmania (Viannia) braziliensis and L. (V.) guyanensis. Mem Inst Oswaldo Cruz 92 : 581–582.
  23. Banuls AL, Jonquieres R, Guerrini F, Le Pont F, Barrera C, Espinel I, Guderian R, Echeverria R, Tibayrenc M, 1999. Genetic analysis of Leishmania parasites in Ecuador: are Leishmania (Viannia) panamensis and Leishmania (V.) guyanensis distinct taxa? Am J Trop Med Hyg 61 : 838–845.
  24. Kelly JM, Law JM, Chapman CJ, Van Eys GJ, Evans DA, 1991. Evidence of genetic recombination in Leishmania. Mol Biochem Parasitol 46 : 253–263.
  25. Mauricio IL, Silk R, Baghaei M, Yeo M, Pratlong F, Zemanova E, Dedet JP, Lukes J, Miles MA, 2006. Leishmania donovani complex: resolving genotypes and haplotypes for polymorphic metabolic enzymes. Int J Parasitol 36 : 757–769.
  26. Schwenkenbecher JM, Wirth T, Schnur LF, Jaffe CL, Schallig H, Al-Jawabreh A, Hamarsheh O, Azmi K, Pratlong F, Schönian G, 2006. Microsatellite analysis reveals that population structure of Leishmania tropica exhibits both clonality and recombination. Int J Parasit 36 : 237–246.
  27. Ravel C, Cortes S, Pratlong F, Morio F, Dedet JP, Campino L, 2006. First report of genetic hybrids between two very divergent Leishmania species: Leishmania infantum and Leishmania major. Int J Parasitol.
  28. Yeo M, Acosta N, Llewellyn M, Sanchez H, Adamson S, Miles GA, Lopez E, Gonzalez N, Patterson JS, Gaunt MW, de Arias AR, Miles MA, 2005. Origins of Chagas disease: Didelphis species are natural hosts of Trypanosoma cruzi I and armadillos hosts of Trypanosoma cruzi II, including hybrids. Int J Parasitol 35 : 225–233.
  29. Zhang WW, Miranda-Verastegui C, Arevalo J, Mdao M, Ward B, Llanos-Cuentas A, Matlashewski G, 2006. Development of a genetic assay to distinguish between Leishmania (Viannia) species on the basis of isoenzyme differences. Clin Infect Dis 42 : 801–809.
  30. Zemanova E, Jirku M, Mauricio IL, Horak A, Miles MA, Lukes J, 2007. The Leishmania donovani complex: genotypes of five metabolic enzymes (ICD, ME, MPI, G6PDH, and FH), new targets for multilocus sequence typing. Int J Parasitol 37 : 149–160.
  31. Jamjoom MB, Ashford RW, Bates PA, Chance ML, Kemp SJ, Watts PC, Noyes HA, 2004. Leishmania donovani is the only cause of visceral leishmaniasis in East Africa; previous descriptions of L. infantum and “L. archibaldi” from this region are a consequence of convergent evolution in the isoenzyme data. Parasitology 129 : 399–409.
  32. Torrico MC, De Doncker S, Arevalo J, Le Ray D, Dujardin JC, 1999. In vitro promastigote fitness of putative Leishmania (Viannia) braziliensis/Leishmania (Viannia) peruviana hybrids. Acta Trop 72 : 99–110.
  33. Almeida MC, Cuba-Cuba CA, Moraes MAP, Miles MA, 1996. Dissemination of Leishmania (Viannia) braziliensis. J Comp Pathol 115 : 311–316.
  34. de Moura TR, Novais FO, Oliveira F, Clarencio J, Noronha A, Barral A, Brodskyn C, de Oliveira CI, 2005. Toward a novel experimental model to study American cutaneous leishmaniasis caused by Leishmania braziliensis. Infect Immun 73 : 5827–5834.
  35. Garcia AL, Kindt A, Quispe-Tintava KW, Bermudez H, Llanos A, Arevalo J, Banuls AL, De Doncker S, Le Ray D, Dujardin JC, 2005. American tegumentary leishmaniasis: antigen-gene polymorphism, taxonomy and clinical pleomorphism. Infect Genet Evol 5 : 109–116.
  36. Rotureau B, Ravel C, Nacher M, Couppie P, Curtet I, Dedet JP, Carme B, 2006. Molecular epidemiology of Leishmania (Viannia) guyanensis in French Guiana. J Clin Microbiol 44 : 468–473.
  37. Cuervo P, Cupolillo E, Nehme N, Hernandez V, Saravia N, Fernandes O, 2004. Leishmania (Viannia): genetic analysis of cutaneous and mucosal strains isolated from the same patient. Exp Parasitol 108 : 59–66.
  38. Nolder D, 2000. Molecular Diversity in the Leishmania Subgenus Viannia. PhD thesis. University of London.
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  • Received : 10 May 2006
  • Accepted : 31 Oct 2006

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