1921
Volume 76, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Because information about genome size in triatomines is scarce and contradictory, we performed DNA quantification by flow cytometry in 13 species belonging to five genera (, , , and ) to infer overall tendencies and phylogenetic associations. The results show that the haploid DNA content of the subfamily Triatominae varies nearly 4-fold, from < 0.7 pg in species (0.6 × 10 bp) to 2.7 pg in (2.6 × 10 bp). Considering that triatomines present similar chromosome numbers, we suggest that genome size differences are the result of variation in the quantity of repetitive DNA sequences localized in hetero and euchromatin. Changes in heterochromatin are particularly important when considering populations or closely related species; in more distant taxa, euchromatic changes also play a role. Our analyses indicate that flow cytometry is a useful tool for population, taxonomic, and evolutionary studies in this subfamily.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.2007.76.516
2007-03-01
2017-09-25
Loading full text...

Full text loading...

/deliver/fulltext/14761645/76/3/0760516.html?itemId=/content/journals/10.4269/ajtmh.2007.76.516&mimeType=html&fmt=ahah

References

  1. Galvão C, Carcavallo RU, Rocha DS, Jurberg J, 2003. A checklist of the current valid species of the subfamily Triatominae Jeannel, 1919 (Hemiptera, Reduviidae) and their geographical distribution, with nomenclatural and taxonomic notes. Zootaxa 202 : 1–36.
  2. Morel CM, Lazdins J, 2003. Chagas disease. Nat Rev Microbiol 1 : 14–15.
  3. WHO, 2002. Control of Chagas disease: Second report of the WHO Expert Committee. World Health Organ Tech Rep Ser 905 : 1–109.
  4. Monteiro FA, Escalante AA, Beard CB, 2001. Molecular tools and triatomine systematics: a public health perspective. Trends Parasitol 17 : 344–347.
  5. Dujardin JP, Schofield CJ, Panzera F, 2002. Los vectores de la Enfermedad de Chagas. Bruxelles: Classe des Sciences Naturelles et Médicales.
  6. Gregory TR, 2005. The Evolution of the Genome. New York: Elsevier.
  7. Evans JD, Gundersen-Rindal D, 2003. Beenomes to Bombyx: Future directions in applied insect genomics. Genome Biol 4 : 107.
  8. Schreiber G, Bogliolo AN, Coelho de Pinho A, 1972. Cytogenetics of Triatominae: caryotype, DNA content, nuclear size and heteropyknosis of autosomes. Rev Brasil Biol 32 : 255–263.
  9. Panzera F, Pérez R, Panzera Y, Alvarez F, Scvortzoff E, Salvatella R, 1995. Karyotype evolution in holocentric chromosomes of three related species of triatomines (Hemiptera-Reduviidae). Chrom Res 3 : 143–150.
  10. Panzera F, Dujardin JP, Nicolini P, Caraccio MN, Rose V, Téllez T, Bermúdez H, Bargues MD, Mas-Coma S, O’Connor JE, Pérez R, 2004. Genomic changes of Chagas Disease vector, South America. Emerg Infect Dis 10 : 438–446.
  11. Panzera F, Ferrandis I, Ramsey J, Ordóñez R, Salazar-Schettino PM, Cabrera M, Monroy MC, Bargues MD, Mas-Coma S, O’Connor JE, Angulo VM, Jaramillo N, Cordón-Rosales C, Gómez D, Pérez R, 2006. Chromosomal variation and genome size support existence of cryptic species of Triatoma dimidiata with different epidemiological importance as Chagas disease vectors. Trop Med Int Health 11 : 1092–1103.
  12. Bargues MD, Klisiowicz DR, Panzera F, Noireau F, Marcilla A, Pérez R, Rojas MG, O’Connor JE, Gonzalez-Candelas F, Galvão C, Jurberg J, Carcavallo RU, Dujardin JP, Mas-Coma S, 2006. Origin and phylogeography of the Chagas disease main vector Triatoma infestans based on nuclear rDNA sequences and genome size. Infect Genet Evol 6 : 46–62.
  13. International Human Genome Sequencing Consortium, 2001. Initial sequencing and analysis of the human genome. Nature 409 : 860–921.
  14. Dolezel J, Bartos J, Voglmayr H, Greilhuber J, 2003. Nuclear DNA content and genome size of trout and human. Cytometry 51A : 127–128.
  15. Panzera F, Scvortzoff E, Pérez R, Panzera Y, Hornos S, Cestau R, Nicolini P, Delgado V, Alvarez F, Mazzella MC, Cossio G, Martínez M, Salvatella R, 1998. Cytogenetics of Triatomines. Carcavallo RU, Girón IG, Jurberg J, Lent H, eds. Atlas of Chagaś Disease Vectors in the Americas. Vol. II. Rio de Janeiro: FIOCRUZ, 621–664..
  16. Panzera F, Pérez R, Nicolini P, Hornos S, Costa J, Borges É, Diotaiuti L, Schofield CJ, 2000. Chromosome homogeneity in populations of Triatoma brasiliensis Neiva 1911 (Hemiptera-Reduviidae-Triatominae). Cad Saude Publica 16 : 83–88.
  17. Pérez R, Hernández M, Caraccio M, Rose V, Valente A, Valente V, Moreno J, Angulo V, Sandoval M, Roldán J, Vargas F, Wolff M, Panzera F, 2002. Chromosomal evolution trends of the genus Panstrongylus (Hemiptera, Reduviidae), vectors of Chagas disease. Infect Genet Evol 2 : 47–56.
  18. Vilhar B, Greilhuber J, Koce JD, Temsch EM, Dermastia M, 2001. Plant genome size measurement with DNA image cytometry. Ann Bot (Lond) 87 : 719–728.
  19. Arumuganathan K, Earle ED, 1991. Estimation of nuclear DNA content of plants by flow cytometry. Plant Mol Biol Rep 9 : 229–233.
  20. Greilhuber J, 2005. Intraspecific variation in genome size in Angiosperms: Identifying its existence. Ann Bot (Lond) 95 : 91–98.
  21. Johnston JS, Bennett MD, Rayburn AL, Galbraith DW, Price HJ, 1999. Reference standards for determination of DNA content of plant nuclei. Am J Bot 86 : 609–613.
  22. Ciudad J, Cid E, Velasco A, Lara JM, Aijón J, Orfao A, 2002. Flow cytometry measurement of the DNA contents of G0/G1 diploid cells from three different teleost fish species. Cytometry 48 : 20–25.
  23. Paula AS, Diotaiuti L, Schofield CJ, 2005. Testing the sister-group relationship of the Rhodniini and Triatomini (Insecta: Hemiptera: Reduviidae: Triatominae). Mol Phyl Ev 35 : 712–718.
  24. Papeschi AG, 1991. DNA content and heterochromatin variation in species of Belostoma (Heteroptera, Belostomatidae). Hereditas 115 : 109–114.
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2007.76.516
Loading
/content/journals/10.4269/ajtmh.2007.76.516
Loading

Data & Media loading...

  • Received : 24 May 2006
  • Accepted : 11 Oct 2006

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error