1921
Volume 76, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

The African malaria vector is polymorphic for alternative arrangements on the left arm of chromosome 2 (2La and 2L+) that are non-randomly distributed with respect to degree of aridity. Detailed studies on the ecological role of inversion 2La have been hindered by the technical demands of traditional karyotype analysis and by sex- and stage-specific limitations on the availability of polytene chromosomes favorable for analysis. Recent molecular characterization of both inversion breakpoints presented the opportunity to develop a polymerase chain reaction (PCR)-based method for karyotype analysis. Here we report the development of this molecular diagnostic assay and the results of extensive field validation. When tested on 765 specimens sampled across Africa, the molecular approach compared favorably with traditional cytologic methods, correctly scoring > 94% of these specimens. By providing ready access to the 2La karyotype, this tool lays groundwork for future studies of the ecological genomics of this medically important species.

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2007-02-01
2017-09-22
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References

  1. Fischer G, Rocha EP, Brunet F, Vergassola M, Dujon B, 2006. Highly variable rates of genome rearrangements between hemiascomycetous yeast lineages. PLoS Genet 2 : e32.
  2. Navarro A, Barton NH, 2003. Chromosomal speciation and molecular divergence—accelerated evolution in rearranged chromosomes. Science 300 : 321–324.
  3. Coluzzi M, Sabatini A, Della Torre A, Di Deco MA, Petrarca V, 2002. A polytene chromosome analysis of the Anopheles gambiae species complex. Science 298 : 1415–1418.
  4. della Torre A, Costantini C, Besansky NJ, Caccone A, Petrarca V, Powell JR, Coluzzi M, 2002. Speciation within Anopheles gambiae—the glass is half full. Science 298 : 115–117.
  5. Ayala FJ, Coluzzi M, 2005. Chromosome speciation: Humans, Drosophila, and mosquitoes. Proc Natl Acad Sci USA 102 (Suppl 1): 6535–6542.
  6. Sharakhov IV, White BJ, Sharakhova MV, Kayondo J, Lobo NF, Santolamazza F, Della Torre A, Simard F, Collins FH, Besansky NJ, 2006. Breakpoint structure reveals the unique origin of an interspecific chromosomal inversion (2La) in the Anopheles gambiae complex. Proc Natl Acad Sci USA 103 : 6258–6262.
  7. Petrarca V, Nugud AD, Ahmed MA, Haridi AM, Di Deco MA, Coluzzi M, 2000. Cytogenetics of the Anopheles gambiae complex in Sudan, with special reference to An. arabiensis: relationships with East and West African populations. Med Vet Entomol 14 : 149–164.
  8. Powell JR, Petrarca V, della Torre A, Caccone A, Coluzzi M, 1999. Population structure, speciation, and introgression in the Anopheles gambiae complex. Parassitologia 41 : 101–113.
  9. Coluzzi M, Sabatini A, Petrarca V, Di Deco MA, 1977. Behavioural divergences between mosquitoes with different inversion karyotypes in polymorphic populations of the Anopheles gambiae complex. Nature 266 : 832–833.
  10. Holt RA, Subramanian GM, Halpern A, Sutton GG, Charlab R, Nusskern DR, Wincker P, Clark AG, Ribeiro JM, Wides R, Salzberg SL, Loftus B, Yandell M, Majoros WH, Rusch DB, Lai Z, Kraft CL, Abril JF, Anthouard V, Arensburger P, Atkinson PW, Baden H, de Berardinis V, Baldwin D, Benes V, Biedler J, Blass C, Bolanos R, Boscus D, Barnstead M, Cai S, Center A, Chatuverdi K, Christophides GK, Chrystal MA, Clamp M, Cravchik A, Curwen V, Dana A, Delcher A, Dew I, Evans CA, Flanigan M, Grundschober-Freimoser A, Friedli L, Gu Z, Guan P, Guigo R, Hillenmeyer ME, Hladun SL, Hogan JR, Hong YS, Hoover J, Jaillon O, Ke Z, Kodira C, Kokoza E, Koutsos A, Letunic I, Levitsky A, Liang Y, Lin JJ, Lobo NF, Lopez JR, Malek JA, McIntosh TC, Meister S, Miller J, Mobarry C, Mongin E, Murphy SD, O’Brochta DA, Pfannkoch C, Qi R, Regier MA, Remington K, Shao H, Sharakhova MV, Sitter CD, Shetty J, Smith TJ, Strong R, Sun J, Thomasova D, Ton LQ, Topalis P, Tu Z, Unger MF, Walenz B, Wang A, Wang J, Wang M, Wang X, Woodford KJ, Wortman JR, Wu M, Yao A, Zdobnov EM, Zhang H, Zhao Q, et al., 2002. The genome sequence of the malaria mosquito Anopheles gambiae. Science 298 : 129–149.
  11. Gillies MT, De Meillon B, 1968. The Anophelinae of Africa South of the Sahara. Johannesburg: South African Institute for Medical Research.
  12. della Torre A, 1997. Polytene chromosome preparation from anopheline mosquitoes. Crampton JM, Beard CB, Louis C, eds. Molecular Biology of Disease Vectors: A Methods Manual. London: Chapman & Hall; 329–336.
  13. Collins FH, Mendez MA, Rasmussen MO, Mehaffey PC, Besansky NJ, Finnerty V, 1987. A ribosomal RNA gene probe differentiates member species of the Anopheles gambiae complex. Am J Trop Med Hyg 37 : 37–41.
  14. Scott JA, Brogdon WG, Collins FH, 1993. Identification of single specimens of the Anopheles gambiae complex by the polymerase chain reaction. Am J Trop Med Hyg 49 : 520–529.
  15. Rozen S, Skaletsky HJ, 2000. Primer3 on the WWW for general users and for biologist programmers. Krawetz S, Misener S, eds. Bioinformatics Methods and Protocols: Methods in Molecular Biology. Totowa, NJ: Humana Press; 365–386.
  16. Andolfatto P, Wall JD, Kreitman M, 1999. Unusual haplotype structure at the proximal breakpoint of In(2L)t in a natural population of Drosophila melanogaster. Genetics 153 : 1297–1311.
  17. Matzkin LM, Merritt T, Zhu CT, Eanes WF, 2005. The structure and population genetics of the breakpoints associated with the cosmopolitan chromosomal inversion In(3R)Payne in Drosophila melanogaster. Genetics 170 : 1143–1152.
  18. Mathiopoulos KD, della Torre A, Predazzi V, Petrarca V, Coluzzi M, 1998. Cloning of inversion breakpoints in the Anopheles gambiae complex traces a transposable element at the inversion junction. Proc Natl Acad Sci USA 95 : 12444–12449.
  19. Lehmann T, Hawley WA, Collins FH, 1996. An evaluation of evolutionary constraints on microsatellite loci using null alleles. Genetics 144 : 1155–1163.
  20. Stump AD, Shoener JA, Costantini C, Sagnon N, Besansky NJ, 2005. Sex-linked differentiation between incipient species of Anopheles gambiae. Genetics 169 : 1509–1519.
  21. Petrarca V, Beier JC, 1992. Intraspecific chromosomal polymorphism in the Anopheles gambiae complex as a factor affecting malaria transmission in the Kisumu area of Kenya. Am J Trop Med Hyg 46 : 229–237.
  22. Coluzzi M, 1992. Malaria vector analysis and control. Parasitol Today 8 : 113–118.
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  • Received : 02 Aug 2006
  • Accepted : 07 Nov 2006

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