Volume 76, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


We detected in blood samples of the wild rodent by xenodiagnosis and a polymerase chain reaction (PCR) using the domestic and wild vectors of Chagas disease, and , respectively. We captured 35 rodents and extracted DNA from blood samples and intestinal contents of vectors fed on . Our results indicate that the percentage of rodents naturally infected with depends on the biologic sample used for PCR and on the vector species for xenodiagnosis. The PCR with blood samples did not detect DNA, but the PCR with intestinal contents showed that both vectors were positive for . The PCR performed with intestinal contents detected four times more -positive than the PCR with intestinal contents (22.9% and 5.7%, respectively). We report the improvement of detection in sylvatic animals by a combination of PCR and xenodiagnosis using sylvatic vectors, especially in disease-endemic areas with low parasitemias in mammals.


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  • Received : 30 May 2006
  • Accepted : 28 Sep 2006

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