1921
Volume 76, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

We detected in blood samples of the wild rodent by xenodiagnosis and a polymerase chain reaction (PCR) using the domestic and wild vectors of Chagas disease, and , respectively. We captured 35 rodents and extracted DNA from blood samples and intestinal contents of vectors fed on . Our results indicate that the percentage of rodents naturally infected with depends on the biologic sample used for PCR and on the vector species for xenodiagnosis. The PCR with blood samples did not detect DNA, but the PCR with intestinal contents showed that both vectors were positive for . The PCR performed with intestinal contents detected four times more -positive than the PCR with intestinal contents (22.9% and 5.7%, respectively). We report the improvement of detection in sylvatic animals by a combination of PCR and xenodiagnosis using sylvatic vectors, especially in disease-endemic areas with low parasitemias in mammals.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.2007.76.324
2007-02-01
2017-09-20
Loading full text...

Full text loading...

/deliver/fulltext/14761645/76/2/0760324.html?itemId=/content/journals/10.4269/ajtmh.2007.76.324&mimeType=html&fmt=ahah

References

  1. Panzera F, Dujardin JP, Nicolini P, Caraccio MN, Rose V, Tellez T, Bermúdez H, Bargues MD, Mas-Coma S, O’Connor JE, Pérez R, 2004. Genomic changes of Chagas disease vector, South America. Emerg Infect Dis 10 : 438–446.
  2. Rozas M, Botto-Mahan M, Coronado X, Ortiz S, Cattan P, Solari A, 2005. Trypanosoma cruzi infection in wild mammals from a Chagasic area of Chile. Am J Trop Med Hyg 73 : 517–519.
  3. Zulantay I, Honores P, Solari A, Apt W, Ortiz S, Osuna A, Rojas A, Lopez B, Sanchez G, 2004. Use of polymerase chain reaction (PCR) and hybridization assays to detect Trypanosoma cruzi in chronic chagasic patients treated with itraconazole or allopurinol. Diagn Microbiol Infect Dis 48 : 253–257.
  4. Russomando G, Rojas de Arias A, Almiron M, Figueredo A, Ferreira M, Morita K, 1996. Trypanosoma cruzi: polymerase chain reaction-based detection in dried feces of Triatoma infestans. Exp Parasitol 83 : 62–66.
  5. Botto-Mahan C, Ortiz S, Rozas M, Cattan P, Solari A, 2005. DNA evidence of Trypanosoma cruzi in the Chilean wild vector Mepraia spinolai. Mem Inst Oswaldo Cruz 100 : 237–239.
  6. Silber M, Búa J, Porcel B, Segura E, Ruiz A, 1997. Trypanosoma cruzi: specific detection of parasites by PCR in infected humans and vectors using a set of primers (BP1/BP2) targeted to a nuclear DNA sequence. Exp Parasitol 85 : 225–232.
  7. Coronado X, Zulantay I, Albrecht H, Rozas M, Apt W, Ortiz S, Rodriguez J, Sanchez G, Solari A, 2006. Variation in Trypanosoma cruzi clonal composition detected in blood patients and xenodiagnosis triatomines: implications in the molecular epidemiology of Chile. Am J Trop Med Hyg 74 : 1008–1012.
  8. Lorca M, Garcia A, Contreras M, Schenone H, Rojas A, 2001. Evaluation of a Triatoma infestans elimination program by the decrease of Trypanosoma cruzi infection frequency in children younger than 10 years, Chile 1991–1998. Am J Trop Med Hyg 65 : 861–864.
  9. Lent H, Wygodzinsky P, 1979. Revision of the triatominae (Hemiptera: Reduviidae) and their significance as vectors of Chagas disease. Bull Am Mus Nat Hist 163 : 130–138.
  10. Frías DA, Henry AA, González CR, 1998. Mepraia gajardoi: a new species of Triatominae (Hemiptera: Reduviidae) from Chile and its comparison with Mepraia spinolai. Rev Chil Hist Nat 71 : 177–188.
  11. Canals M, Solis R, Valderas J, Ehrenfeld M, Cattan PE, 1997. Preliminary studies on temperature selection and activity cycles Triatoma infestans and T. spinolai (Heteroptera: Reduviidae) Chilean vectors of Chagas disease. J Med Entomol 34 : 11–17.
  12. Barreto M, 1964. Reservatórios do Trypanosoma cruzi nas Américas. Rev Brasil Malariol D Trop 16 : 27.
  13. Whiting C, 1946. Contribución al estudio de las reservas de parásitos en la enfermedad de Chagas en Chile. I. Primeros hallazgos en Chile de mamíferos silvestres naturalmente infectados por el Trypanosoma cruzi. Rev Chil Hig Med Prev 8 : 69–102.
  14. Durán J, Videla M, Apt W, 1989. Enfermedad de Chagas en una comunidad de pequeños mamíferos simpátricos de la reserva nacional de las Chinchillas. Parasitol al Día 13 : 15–20.
  15. Jimenez J, Lorca M, 1990. Trypanosomiasis americana en vertebrados silvestres y su relación con el vector Triatoma spinolai. Arch Med Vet 22 : 179–183.
  16. di Castri F, Hajek ER, 1976. Bioclimatología de Chile. Santiago: Ediciones de la Universidad Católica de Chile.
  17. Wincker P, Britto C, Pereira JB, Cardoso MA, Oeleman O, Morel CM, 1994. Use of a simplified polymerase chain reaction procedure to detect Trypanosoma cruzi in blood samples from chronic chagasic patients in a rural endemic area. Am J Trop Med Hyg 51 : 771–777.
  18. Solari A, Campillay R, Ortiz S, Wallace A, 2001. Identification of Trypanosoma cruzi genotypes circulating in Chilean chagasic patients. Exp Parasitol 97 : 226–233.
  19. Schijman AG, Altcheh J, Burgos JM, Biancardi M, Bisio M, Levin MJ, Freilij H, 2003. Aetiological treatment of congenital Chagas disease diagnosed and monitored by the polymerase chain reaction. J Antimicrob Chemother 52 : 441–449.
  20. Schenone H, 1999. Xenodiagnosis. Mem Inst Oswaldo Cruz 94 : 289–294.
  21. Kollien AH, Schaub GA, 2000. The development of Trypanosoma cruzi in Triatominae. Parasitol Today 16 : 381–387.
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2007.76.324
Loading
/content/journals/10.4269/ajtmh.2007.76.324
Loading

Data & Media loading...

  • Received : 30 May 2006
  • Accepted : 28 Sep 2006

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error