1921
Volume 76, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

response to chloroquine, monodesethylamodiaquine, mefloquine, lumefantrine, and dihydroartemisinin was assessed by the radioisotopic microtest in Yaoundé, Cameroon, during 2000–2004 and compared with our previous data obtained during 1996–1999. Based on the cut-off value of 100 nmol/L, 36.3% of isolates were chloroquine-susceptible ( = 175; geometric mean IC, 40.3 nmol/L) and 63.7% were chloroquine-resistant ( = 307; geometric mean IC, 211 nmol/L). There was no significant difference ( > 0.05) in the mean ICs from 1996 to 2004, but a significant linear trend ( < 0.05) toward an increased proportion of chloroquine-resistant isolates was observed from 1996 (49%) to 2004 (69%). All chloroquine-susceptible isolates and most chloroquine-resistant isolates were susceptible to monodesethylamodiaquine (i.e., IC < 60 nmol/L). Despite the positive correlation between chloroquine and monodesethylamodiaquine ( = 0.739, < 0.05), the ICs for monodesethylamodiaquine remained stable during 1997–2004, with no increase in the proportion of monodesethylamodiaquine-resistant isolates. Mefloquine, lumefantrine, and dihydroartemisinin were equally active against the chloroquine-susceptible and chloroquine-resistant parasites. The responses to these three drugs were positively correlated, and a significant decrease ( < 0.05) in the mean ICs was observed during the study period compared with our earlier data in 1997–1999, probably because of their inverse relationship with chloroquine response. The results were in general agreement with the response to chloroquine and amodiaquine. drug susceptibility assay is a useful, complementary laboratory tool for determining the trend of response to drugs for which there is still no established molecular marker and may serve as an early warning system for emerging drug resistance.

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  • Received : 23 Mar 2006
  • Accepted : 04 Sep 2006

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