1921
Volume 76, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Dengue infection is an important public health issue worldwide. The ChimeriVax™-Dengue (CYD) vaccine uses yellow fever (YF) 17D vaccine as a live vector. Dendritic cells (DCs) play a key role in initiating immune responses and could be an important primary target of dengue infection. We investigated the consequences of CYD infection of DCs on their activation/maturation and cytokine production. In CYD-infected DCs, we observed an up-regulation of HLA-DR, CD80, CD86, and CD83. Cells exposed to CYD secreted type I interferons, monocyte chemoattractant protein 1 (MCP-1)/CC chemokine ligand 2 (CCL-2), interleukin-6 (IL-6), and low amounts of tumor necrosis factor-α (TNF-α), but no IL-10, IL-12, or IL-1α. Parental dengue viruses induced a similar array of cytokines, but more TNF-α, less IL-6, and less MCP-1/CCL-2 than induced by CYD. Chimeras thus induced DCs maturation and a controlled response accompanied by limited inflammatory cytokine production and consistent expression of anti-viral interferons, in agreement with clinical observations of safety and immunogenicity.

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2007-01-01
2017-11-24
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  • Received : 15 Feb 2006
  • Accepted : 26 Sep 2006

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