1921
Volume 76, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

We assessed the status of point mutations associated with chloroquine resistance in codon 76 and in codon 86 among isolates from symptomatic patients in 3 sites in Madagascar. The susceptibility of isolates to quinoline-containing drugs was also determined. All isolates ( = 117) successfully typed were wild-type, except one from Tsiroanomandidy (1 of 27). However, 67.5% (95% CI: 58.2–75.9%) of these isolates contained mutant 86Y. The N86Y mutation is associated with higher mefloquine susceptibility, but it did not affect the sensitivity of parasites to chloroquine or quinine. Our findings demonstrate that mutant are prevalent in Madagascar and confirm the low prevalence of mutant after 60 years of chloroquine use. They provide additional field-based evidence for increased mefloquine susceptibility in mutant and are suggestive of the intrahost selection of mutant parasites.

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  • Received : 19 Dec 2006
  • Accepted : 31 Jan 2007

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