Volume 75, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Blood-feeding obtained its fills from immune chickens in 15 min, but it needed 40 min for feeding upon non-immune chickens. High-titer specific IgGs and skin reactivity against saliva antigens were elicited in immune chickens. Fluorescence-labeled leukocytes from non-immune or immune chickens were used to determine sources of blood drawn by equal numbers of triatomines distributed in separate compartments of a hut-like box. It was shown that 64.4 ± 4.7% of the reduviids were captured in the immune chicken room; 35.6 ± 4.5% were present in the non-immune chicken dwelling, and these differences were statistically significant ( < 0.001). Furthermore, feeding upon immune birds reached the adult stage 40 days before those feeding upon non-immune birds, and differences were statistically significant. These results appear to have a broad epidemiologic significance as for spreading enzootics; hence, the immunologic status of vertebrate host populations appears to favor as the main transmitter of .


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  1. Carcavallo RU, Jurberg J, Galindez Giron I, Lent H, 1997. Atlas of Chagas’ disease vectors in the Americas. Editora Fiocruz, Rio de Janeiro.
  2. Gaunt M, Miles M, 2000. The ecotopes and evolution of triatomine bugs (Triatominae) and their associated trypanosomes. Mem Inst Oswaldo Cruz 95 : 557–565. [Google Scholar]
  3. Lyman DF, Monteiro FA, Escalante AA, Cordon-Rosales C, Wesson DM, Dujardin JP, Beard CB, 1999. Mitochondrial DNA sequence variation among triatomine vectors of Chagas’ disease. Am J Trop Med Hyg 60 : 377–386. [Google Scholar]
  4. World Health Organization, 2002. Control of Chagas’ disease: second report of a WHO Expert Committee. WHO Tech Rep Series 905 : 1–109. [Google Scholar]
  5. Correa-Oliveira R, Gomes J, Lemos EM, Cardoso GM, Reis DD, Adad S, Crema E, Martins-Filho OA, Costa MO, Gazzinelli G, Bahia-Oliveira LM, 1999. The role of the immune response on the development of severe clinical forms of human Chagas disease. Mem Inst Oswaldo Cruz 94 : 253–255. [Google Scholar]
  6. Gomes JA, Bahia-Oliveira LM, Rocha MO, Martins-Filho OA, Gazzinelli G, Correa-Oliveira R, 2003. Evidence that development of severe cardiomyopathy in human Chagas’ disease is due to a Th1-specific immune response. Infect Immun 71 : 1185–1193. [Google Scholar]
  7. Wang H, Nuttall PA, 1995. Excretion of host immunoglobulin in tick saliva and detection of IgG-binding proteins in tick hae-molymph and salivary glands. Parasitology 110 : 363. [Google Scholar]
  8. Titus RG, Ribeiro JMC, 1988. Salivary gland lysates from the sand fly Lutzomyia longipalpis enhance Leishmania infectivity. Science 239 : 1306–1308. [Google Scholar]
  9. Belkaid Y, Valenzuela JG, Kamhawi S, Rowton E, Sacks DL, Ribeiro JM, 2000. Delayed-type hypersensitivity to Phlebotomus papatasi sand fly bite: an adaptive response induced by the fly? Proc Natl Acad Sci USA 97 : 6704–6709. [Google Scholar]
  10. Valenzuela JG, Belkaid Y, Garfield MK, Mendez S, Kamhowis S, Rowton E, Sacks DL, Ribeiro JM, 2001. Toward a defined anti-Leishmania vaccine targeting vector antigens: characterization of a protective salivary protein. J Exp Med 194 : 331–342. [Google Scholar]
  11. Faudry E, Lozzi SP, Santana JM, D’Souza-Ault M, Kieffer S, Felix CR, Ricart CA, Sousa MV, Vernet T, Teixeira AR, 2004. Triatoma infestans apyrases belong to the 5′-nucleotidase family. J Biol Chem 279 : 19607–19613. [Google Scholar]
  12. Ribeiro JM, 1995. Blood-feeding arthropods: live syringe or invertebrate pharmacologists? Infect Agents Disease 4 : 143–152. [Google Scholar]
  13. Wood SF, 1942. Reactions of man to the feeding of reduviid bugs. J Parasitol 28 : 43–49. [Google Scholar]
  14. Teo SK, Cheah JS, 1973. Severe reaction to the bite of the tri-atomid bug (Triatoma rubrofasciata) in Singapore. J Trop Med Hyg 76 : 161–162. [Google Scholar]
  15. Mott KE, França JT, Barrett TV, Hoff R, Oliveira TS, Sherlock IA, 1980. Cutaneous allergic reactions to Triatoma infestans after xenodiagnosis. Mem Inst Oswaldo Cruz 75 : 3–10. [Google Scholar]
  16. Chapman MD, Marshall NA, Saxon A, 1986. Identification and partial purification of species-specific allergens from Triatoma protracta (Heteroptera: Reduviidae). J Allerg Clin Immunol 78 : 436–442. [Google Scholar]
  17. Golden DB, Marsh DG, Kagey-Sobotka A, Freidhoff L, Szklo M, Valentine MD, Lichtenstein LM, 1989. Epidemiology of insect venom sensitivity. JAMA 262 : 240–244. [Google Scholar]
  18. Nascimento RJ, Santana JM, Lozzi SP, Araujo CN, Teixeira AR, 2001. Human IgG1 and IgG4: the main antibodies against Tri-atoma infestans (Hemiptera: Reduviidae) salivary gland proteins. Am J Trop Med Hyg 65 : 219–226. [Google Scholar]
  19. Schweigmann NJ, Pietrokovsky S, Bottazzi V, Conti O, Wisnivesky-Colli C, 1995. Interaction between Didelphis albiventris and Triatoma infestans in relation to Trypanosoma cruzi transmission. Mem Inst Oswaldo Cruz 90 : 679–682. [Google Scholar]
  20. Hasek M, Knizetová F, Mervartová H, 1966. Syngenic lines of chickens. I. Inbreeding and selection by means of skin grafts and tests for erythrocyte antivens in C line chickens. Folia Biol 12 : 315–342. [Google Scholar]
  21. Brener Z, Gazzinelli RT, 1997. Immunological control of Trypanosoma cruzi infection and pathogenesis of Chagas’ disease. Int Arch Allerg Immunol 114 : 103–110. [Google Scholar]
  22. Mathews GV, Sidjanski S, Vanderberg JP, 1996. Inhibition of mosquito salivary gland apyrase activity by antibodies produced in mice immunized by bites of Anopheles stephensi mosquitoes. Am J Trop Med Hyg 55 : 417–423. [Google Scholar]
  23. Wikel SK, 1996. Host immunity to ticks. Annu Rev Entomol 41 : 1–22. [Google Scholar]
  24. Ghosh KN, Mukhopadhyay J, 1998. The effect of anti-sandfly saliva antibodies on Phlebotomus argentipes and Leishmania donovani. Int J Parasitol 28 : 275–281. [Google Scholar]
  25. Lukashevich ED, Mostovisk MB, 2003. Hematophagous insects in the fossil record. Paleontol J 37 : 153–161. [Google Scholar]
  26. Valenzuela JG, Charlab R, Gonzalez EC, de Miranda-Santos IKF, Marinotti O, Francischetti IMB, Ribeiro JM, 2002. The D7 family of salivary proteins in blood sucking diptera. Ins Mol Biol 11 : 149–155. [Google Scholar]
  27. Ribeiro JM, 2004. Bugs, blood, and blisters. J Invest Dermatol 123 : xvi. [Google Scholar]
  28. Silva F, Gomes R, Prates JD, Miranda C, Andrade B, Barral-Neto M, Barral A, 2005. Inflammatory cell infiltration and high antibody production in Balb/c mice caused by natural exposure to Lutzomyia longipalpis bites. Am J Trop Med Hyg 72 : 94–98. [Google Scholar]
  29. Watson FL, Puttmann-Holgado R, Thomas F, Lamar DL, Hughes M, Kondo M, Rebel VI, Schmucker D, 2005. Extensive diversity of Ig-superfamily proteins in the immune system of insects. Science 309 : 1874–1878. [Google Scholar]
  30. Volf P, Grubhoffer L, Hosek P, 1993. Characterization of salivary gland antigens of Triatoma infestans and antigen-specific serum antibody response in mice exposed to bites of T. infestans. Vet Parasitol 47 : 327–337. [Google Scholar]
  31. Andersen JF, Gudderra NP, Francischetti IM, Ribeiro JM, 2005. The role of salivary lipocalins in blood feeding by Rhodnius prolixus. Arch Ins Biochem Physiol 58 : 97–105. [Google Scholar]
  32. Gudderra NP, Ribeiro JM, Andersen JF, 2005. Structural determinants of factor IX(a) binding in nitrophorin 2, a lipocalin inhibitor of the intrinsic coagulation pathway. J Biol Chem 280 : 25022–25028. [Google Scholar]

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  • Received : 23 Mar 2006
  • Accepted : 13 Jun 2006

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