Volume 75, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Little is known about the changes in gut resident flora during amebic colitis and amebic liver abscess (ALA) caused by infection. Fecal samples from ALA patients, from healthy negative and positive (asymptomatic) individuals, and from pre- and post-metronidazole–treated healthy volunteers and pus samples from ALA patients were tested for the presence of various bacterial genera using 16S rRNA–based primers. Statistically significant reduction in due to infection was observed in asymptomatic individuals and ALA patients. On the other hand, reduction in , , and in the same samples was due to metronidazole treatment. Two anaerobic genera, viz. and , were detected in ALA pus samples, and this observation is unprecedented. In addition, PCR revealed metronidazole resistance genes in fecal and pus samples of metronidazole-treated individuals. Re-examination of the ameba-bacterium relationship in amebiasis is suggested.


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  • Received : 03 Jun 2006
  • Accepted : 14 Jul 2006

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