Volume 75, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


This study aims to typify the (sub)lineage(s) in umbilical cord blood of congenitally infected Bolivian newborns, using PCR amplifications of “Region Markers”, mini-exon or kDNA fragments followed by hybridization or sequencing. New probes were also designed to distinguish three variants within the TcIId sublineage. The IIb, IId, or IIe sublineages, as well as different variants of the IId sublineage, were detected in infected neonates, whereas mixed infections were not found. The frequencies of the IId sublineage were similar in neonates (95.1%) and adults of the same area (94.1%). The IId-infected newborns displayed either asymptomatic, or severe and fatal clinical forms of congenital Chagas disease, as well as low or high parasitemia. Altogether these data show that DNA polymorphism, based on the presently available markers, is not associated with the occurrence of congenital infection or the development of severe clinical forms of congenital Chagas disease.


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  1. Carlier Y, Pinto Dias JC, Ostermayer Luquetti AO, Hontebeyrie M, Torrico F, Truyens C, 2002.Trypanosomiase américaine ou maladie de Chagas. In: Elsevier SAS, editor. Encycl Méd Chir, Maladies Infectieuses. Paris: 8-505-A-20, 21 p.
  2. Schenone H, Gaggero M, Sapunar J, Contreras MC, Rojas A, 2001. Congenital Chagas disease of second generation in Santiago, Chile. Report of two cases. Rev Inst Med Trop Sao Paulo 43 : 231–232. [Google Scholar]
  3. Carlier Y, Torrico F, 2003. Congenital infection with Trypanosoma cruzi: from mechanisms of transmission to strategies for diagnosis and control. Rev Soc Bras Med Trop 36 : 767–771. [Google Scholar]
  4. Torrico F, Alonso-Vega C, Suarez E, Rodriguez P, Torrico MC, Dramaix M, Truyens C, Carlier Y, 2004. Maternal Trypanosoma cruzi infection, pregnancy outcome, morbidity, and mortality of congenitally infected and non-infected newborns in Bolivia. Am J Trop Med Hyg 70 : 201–209. [Google Scholar]
  5. Hermann E, Truyens C, Alonso-Vega C, Even J, Rodriguez P, Berthe A, Gonzalez-Merino E, Torrico F, Carlier Y, 2002. Human fetuses are able to mount an adultlike CD8 T-cell response. Blood 100 : 2153–2158. [Google Scholar]
  6. Hermann E, Truyens C, Alonso-Vega C, Rodriguez P, Berthe A, Torrico F, Carlier Y, 2004. Congenital transmission of Trypanosoma cruzi is associated with maternal enhanced parasitemia and decreased production of interferon-gamma in response to parasite antigens. J Infect Dis 189 : 1274–1281. [Google Scholar]
  7. Revollo S, Oury B, Laurent JP, Barnabe C, Quesney V, Carriere V, Noel S, Tibayrenc M, 1998. Trypanosoma cruzi: impact of clonal evolution of the parasite on its biological and medical properties. Exp Parasitol 89 : 30–39. [Google Scholar]
  8. Buscaglia CA, Di Noia JM, 2003. Trypanosoma cruzi clonal diversity and the epidemiology of Chagas’ disease. Microbes Infect 5 : 419–427. [Google Scholar]
  9. Macedo AM, Machado CR, Oliveira RP, Pena SD, 2004. Trypanosoma cruzi: genetic structure of populations and relevance of genetic variability to the pathogenesis of chagas disease. Mem Inst Oswaldo Cruz 99 : 1–12. [Google Scholar]
  10. Andrade SG, 1982. The influence of the strain of Trypanosoma cruzi in placental infections in mice. Trans R Soc Trop Med Hyg 76 : 123–128. [Google Scholar]
  11. Gonzalez Cappa SM, Mirkin GA, Solana ME, Tekiel VS, 1999. Patologia for Trypanosoma cruzi: cepa dependiente? Medicina (B Aires) 59 (Suppl 2): 69–74. [Google Scholar]
  12. Moreno EA, Rivera IM, Moreno SC, Alarcon ME, Lugo-Yarbuh A, 2003. Transmisión vertical de Trypanosoma cruzi en ratas Wistar durante la fase aguda de la infección. Invest Clin 44 : 241–254. [Google Scholar]
  13. Recommendations from a satellite meeting, 1999. Mem Inst Oswaldo Cruz 94 Suppl 1: 429–432. [Google Scholar]
  14. Tibayrenc M, Ayala FJ, 1999. Evolutionary genetics of Trypanosoma and Leishmania. Microbes Infect 1 : 465–472. [Google Scholar]
  15. Barnabe C, Brisse S, Tibayrenc M, 2000. Population structure and genetic typing of Trypanosoma cruzi, the agent of Chagas disease: a multilocus enzyme electrophoresis approach. Parasitology 120 : 513–526. [Google Scholar]
  16. Brisse S, Henriksson J, Barnabe C, Douzery EJ, Berkvens D, Serrano M, De Carvalho MR, Buck GA, Dujardin JC, Tibayrenc M, 2003. Evidence for genetic exchange and hybridization in Trypanosoma cruzi based on nucleotide sequences and molecular karyotype. Infect Genet Evol 2 : 173–183. [Google Scholar]
  17. Gaunt MW, Yeo M, Frame IA, Stothard JR, Carrasco HJ, Taylor MC, Mena SS, Veazey P, Miles GA, Acosta N, de Arias AR, Miles MA, 2003. Mechanism of genetic exchange in American trypanosomes. Nature 421 : 936–939. [Google Scholar]
  18. Sturm NR, Vargas NS, Westenberger SJ, Zingales B, Campbell DA, 2003. Evidence for multiple hybrid groups in Trypanosoma cruzi. Int J Parasitol 33 : 269–279. [Google Scholar]
  19. Westenberger SJ, Barnabe C, Campbell DA, Sturm NR, 2005. Two hybridization events define the population structure of Trypanosoma cruzi. Genetics 171 : 527–543. [Google Scholar]
  20. Yeo M, Acosta N, Llewellyn M, Sanchez H, Adamson S, Miles GA, Lopez E, Gonzalez N, Patterson JS, Gaunt MW, de Arias AR, Miles MA, 2005. Origins of Chagas disease: Didelphis species are natural hosts of Trypanosoma cruzi I and armadillos hosts of Trypanosoma cruzi II, including hybrids. Int J Parasitol 35 : 225–233. [Google Scholar]
  21. Breniere SF, Bosseno MF, Telleria J, Bastrenta B, Yacsik N, Noireau F, Alcazar JL, Barnabe C, Wincker P, Tibayrenc M, 1998. Different behavior of two Trypanosoma cruzi major clones: transmission and circulation in young Bolivian patients. Exp Parasitol 89 : 285–295. [Google Scholar]
  22. Solari A, Campillay R, Ortiz S, Wallace A, 2001. Identification of Trypanosoma cruzi genotypes circulating in Chilean chagasic patients. Exp Parasitol 97 : 226–233. [Google Scholar]
  23. Breniere SF, Bosseno MF, Noireau F, Yacsik N, Liegeard P, Aznar C, Hontebeyrie M, 2002. Integrate study of a Bolivian population infected by Trypanosoma cruzi, the agent of Chagas disease. Mem Inst Oswaldo Cruz 97 : 289–295. [Google Scholar]
  24. Torres JP, Ortiz S, Munoz S, Solari A, 2004. Trypanosoma cruzi isolates from Chile are heterogeneous and composed of mixed populations when characterized by schizodeme and Southern analyses. Parasitology 128 : 161–168. [Google Scholar]
  25. Virreira M, Torrico F, Truyens C, Alonso-Vega C, Solano M, Carlier Y, Svoboda M, 2003. Comparison of polymerase chain reaction methods for reliable and easy detection of congenital Trypanosoma cruzi infection. Am J Trop Med Hyg 68 : 574–582. [Google Scholar]
  26. Torrico MC, Solano M, Guzman JM, Parrado R, Suarez E, Alonso-Vega C, Truyens C, Carlier Y, 2005. Estimación de la parasitemia en la infección humana por T. cruzi las altas parasitemias están asociadas con la severa y fatal enfermedad de Chagas congénita. Rev Soc Bras Med Trop 38 : 58–61. [Google Scholar]
  27. Barnabe C, Neubauer K, Solari A, Tibayrenc M, 2001. Trypanosoma cruzi: presence of the two major phylogenetic lineages and of several lesser discrete typing units (DTUs) in Chile and Paraguay. Acta Trop 78 : 127–137. [Google Scholar]
  28. Higo H, Miura S, Horio M, Mimori T, Hamano S, Agatsuma T, Yanagi T, Cruz-Reyes A, Uyema N, Rojas de Arias A, Matta V, Akahane H, Hirayama K, Takeuchi T, Tada I, Himeno K, 2004. Genotypic variation among lineages of Trypanosoma cruzi and its geographic aspects. Parasitol Int 53 : 337–344. [Google Scholar]
  29. Brisse S, Dujardin JC, Tibayrenc M, 2000. Identification of six Trypanosoma cruzi lineages by sequence-characterised amplified region markers. Mol Biochem Parasitol 111 : 95–105. [Google Scholar]
  30. Wincker P, Bosseno MF, Britto C, Yaksic N, Cardoso MA, Morel CM, Breniere SF, 1994. High correlation between Chagas’ disease serology and PCR-based detection of Trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area. FEMS Microbiol Lett 124 : 419–423. [Google Scholar]
  31. Souto RP, Fernandes O, Macedo AM, Campbell DA, Zingales B, 1996. DNA markers define two major phylogenetic lineages of Trypanosoma cruzi. Mol Biochem Parasitol 83 : 141–152. [Google Scholar]
  32. Fernandes O, Souto RP, Castro JA, Pereira JB, Fernandes NC, Junqueira AC, Naiff RD, Barrett TV, Degrave W, Zingales B, Campbell DA, Coura JR, 1998. Brazilian isolates of Trypanosoma cruzi from humans and triatomines classified into two lineages using mini-exon and ribosomal RNA sequences. Am J Trop Med Hyg 58 : 807–811. [Google Scholar]
  33. Telleria J, Lafay B, Virreira M, Barnabe C, Tibayrenc M, Svoboda M, 2006. Trypanosoma Cruzi: sequence analysis of the variable region of kinetoplast minicircles. Exp Parasitol. (in press).
  34. Veas F, Breniere SF, Cuny G, Brengues C, Solari A, Tibayrenc M, 1991. General procedure to construct highly specific kDNA probes for clones of Trypanosoma cruzi for sensitive detection by polymerase chain reaction. Cell Mol Biol 37 : 73–84. [Google Scholar]
  35. Breniere SF, Bosseno MF, Revollo S, Rivera MT, Carlier Y, Tibayrenc M, 1992. Direct identification of Trypanosoma cruzi natural clones in vectors and mammalian hosts by polymerase chain reaction amplification. Am J Trop Med Hyg 46 : 335–341. [Google Scholar]
  36. Vago AR, Andrade LO, Leite AA, d’Avila RD, Macedo AM, Adad SJ, Tostes S Jr, Moreira MC, Filho GB, Pena SD, 2000. Genetic characterization of Trypanosoma cruzi directly from tissues of patients with chronic Chagas disease: differential distribution of genetic types into diverse organs. Am J Pathol 156 : 1805–1809. [Google Scholar]
  37. Franco DJ, Vago AR, Chiari E, Meira FC, Galvao LM, Machado CR, 2003. Trypanosoma cruzi: mixture of two populations can modify virulence and tissue tropism in rat. Exp Parasitol 104 : 54–61. [Google Scholar]
  38. Devera R, Fernandes O, Coura JR, 2003. Should Trypanosoma cruzi be called “cruzi” complex? a review of the parasite diversity and the potential of selecting population after in vitro culturing and mice infection. Mem Inst Oswaldo Cruz 98 : 1–12. [Google Scholar]
  39. de Lana M, da Silveira PA, Barnabe C, Quesney V, Noel S, Tibayrenc M, 1998. Trypanosoma cruzi: compared vectorial transmissibility of three major clonal genotypes by Triatoma infestans. Exp Parasitol 90 : 20–25. [Google Scholar]
  40. da Silveira PA, de Lana M, Britto C, Bastrenta B, Tibayrenc M, 2000. Experimental Trypanosoma cruzi biclonal infection in Triatoma infestans: detection of distinct clonal genotypes using kinetoplast DNA probes. Int J Parasitol 30 : 843–848. [Google Scholar]
  41. Bittencourt AL, Mota E, Povoa M, 1985. Isoenzyme characterization of Trypanosoma cruzi from congenital cases of Chagas’ disease. Ann Trop Med Parasitol 79 : 393–396. [Google Scholar]
  42. Munoz S, Lorca M, Munoz P, Solari A, 1994. Highly homogeneous Trypanosoma cruzi populations in a low endemic region for Chagas disease: importance in the pathogenesis of Chagas disease in Chile. Rev Med Chil 122 : 1231–1238. [Google Scholar]
  43. Breniere SF, Carrasco R, Revollo S, Aparicio G, Desjeux P, Tibayrenc M, 1989. Chagas’ disease in Bolivia: clinical and epidemiological features and zymodeme variability of Trypanosoma cruzi strains isolated from patients. Am J Trop Med Hyg 41 : 521–529. [Google Scholar]
  44. Coura JR, Junqueira AC, Fernandes O, Valente SA, Miles MA, 2002. Emerging Chagas disease in Amazonian Brazil. Trends Parasitol 18 : 171–176. [Google Scholar]
  45. Campbell DA, Westenberger SJ, Sturm NR, 2004. The determinants of Chagas disease: connecting parasite and host genetics. Curr Mol Med 4 : 549–562. [Google Scholar]
  46. Monis PT, Giglio S, Keegan AR, Andrew Thompson RC, 2005. Emerging technologies for the detection and genetic characterization of protozoan parasites. Trends Parasitol 21 : 340–346. [Google Scholar]
  47. Szmaragd C, Nichols RA, Balloux F, 2006. A novel approach to characterise pathogen candidate genetic polymorphisms involved in clinical outcome. Infect Genet Evol 6 : 38–45. [Google Scholar]
  48. Cruz-Robles D, Reyes PA, Monteon-Padilla VM, Ortiz-Muniz AR, Vargas-Alarcon G, 2004. MHC class I and class II genes in Mexican patients with Chagas disease. Hum Immunol 65 : 60–65. [Google Scholar]
  49. Colorado IA, Acquatella H, Catalioti F, Fernandez MT, Layrisse Z, 2000. HLA class II DRB1, DQB1, DPB1 polymorphism and cardiomyopathy due to Trypanosoma cruzi chronic infection. Hum Immunol 61 : 320–325. [Google Scholar]
  50. Torrico F, Alonso-Vega C, Suarez E, Tellez T, Brutus P, Torrico MC, Schneider D, Tryens C, Carlier Y, 2006. Are maternal re-infections with Trypanosoma cruzi associated with higher morbidity and mortality of congenital Chagas disease? Trop Med Intern Health 11 : 628–635. [Google Scholar]
  51. Carlier Y, 2005. Factores y mecanismos involucrados en la transmisión y el desarrollo de la infección congénita por Trypanosoma cruzi. Rev Soc Bras Med Trop 38 : 105–107. [Google Scholar]

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  • Received : 30 Mar 2006
  • Accepted : 26 May 2006

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