1921
Volume 75, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Approximately 55% of malaria infections in the Guyana Amazon region are attributed to while the other 45% are attributed to non-falciparum, mostly . However, little is known about the strain types circulating in the region. Using PCR for detection and two genetic markers specific to to detect the polymorphic circumsporozoite protein (CSP) and the conserved 19-kDa region of the merozoite surface protein-1 (MSP-1), we investigated the overall strain distribution and population diversity within in isolates collected from the blood of infected individuals in the interior Amazon region of Guyana, South America. Out of a total of 250 samples positive for , was detected in 30% (76/250) and was detected in 76% (189/250). Mixed infections containing both and constituted 6% (15/250) of the total positive samples. Further analysis of strains showed that 92% (56/61) of the samples hybridized with a probe specific to type VK210, 39% (24/61) hybridized with a probe specific for type VK247, and 25% (15/61) hybridized with a probe specific for the -like CS genotype. DNA sequencing of the 19-kDa C-terminal domain in block 13 of MSP-1 amplified from 61 samples from patients infected with demonstrated that this region is highly conserved, and all samples were identical at the nucleotide level to the Belem and Salvador-1 types. No synonymous or nonsynonymous mutations were observed in this region of the gene, indicating that current vaccine-development efforts based on the MSP-1 fragment would be applicable in Guyana.

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  • Received : 07 Apr 2006
  • Accepted : 20 Jul 2006

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