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Volume 75, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Correlation studies on the drug response of field isolates of and molecular markers for drug resistance are becoming important as many malaria control programs abandon monotherapies and resort to combination therapies. The standardization and optimization of the drug sensitivity assay are one of the prerequisites for validating molecular markers in the field. The present study was designed to assess and compare the growth of freshly obtained isolates for at least the first erythrocytic cycle in various culture media and determine the response to chloroquine in alternative media. Parasite growth was consistently higher in Dulbecco’s modified Eagle’s medium (DME)–human serum, Iscove’s modified Dulbecco’s medium (IMDM)–human serum, RPMI 1640 medium–goat serum, and a serum-free medium containing 1:1 (v/v) mixture of IMDM and F-12 supplemented with an ammonium sulfate fraction of adult bovine serum than in RPMI 1640 medium–human serum mixture. The level of chloroquine response determined in human serum-supplemented DME, IMDM, and RPMI 1640 media did not differ significantly ( > 0.05) from the control (RPMI 1640–human serum). This study suggests that alternative media may be used to optimize parasite growth during the critical initial phase of transition from to conditions. The capacity of these media to support long-term cultivation of requires further investigation.

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2006-11-01
2017-11-23
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  • Received : 01 May 2005
  • Accepted : 02 Aug 2006

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