Volume 75, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Tree squirrels ( spp.) have exhibited high seroprevalence rates, suggesting that they are commonly exposed to West Nile virus (WNV). Many characteristics of WNV infections in tree squirrels, such as the durations and levels of viremia, remain unknown. To better understand WNV infections in fox squirrels (), we subcutaneously inoculated fourteen fox squirrels with WNV. Peak viremias ranged from 10 plaque-forming units (PFU)/mL of serum on day 2 post-infection (DPI) to 10 PFU/mL on 3 DPI, although viremias varied between individuals. Oral secretions of some fox squirrels were positive for WNV viral RNA, occasionally to moderate levels (10 PFU equivalent/swab). WNV PFU equivalents in organs were low or undetectable on 12 DPI; gross and histologic lesions were rare. The viremia profiles of fox squirrels indicate that they could serve as amplifying hosts in nature. In addition, viral RNA in the oral cavity and feces indicate that this species could contribute to alternative WNV transmission in suburban communities.


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  • Received : 22 Apr 2006
  • Accepted : 14 Jun 2006

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