Volume 75, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Polymerase chain reaction integrated with sequence analysis was carried out to investigate the natural infection in ticks and rodents from a forest area of Jilin Province, China. Four (4.0%) of 100 and 2 (0.7%) of 286 ticks collected by flagging vegetation were positive. Nine (8.8%) of 102 rodents were infected, as well as 2 (2.8%) of 71 parasitizing on 25 rodents. The nucleotide sequences of 1442-bp 16S rRNA gene amplified from rodents and ticks were identical to each other and to that previously reported in Heilongjiang Province of China (GenBank accession no. AF205140), but different from those of other countries. The sequences of 357-bp partial citrate synthase gene from the above specimens were homologic, and varied from known agents. These findings add new information on the ecologic features of and indicate the threat of anaplasmosis in the area.


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  1. Dumler JS, Barbet AF, Bekker CP, Dasch GA, Palmer GH, Ray SC, Rikihisa Y, Rurangirwa FR, 2001. Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and “HGE agent” as subjective synonyms of Ehrlichia phagocytophila. Int J Syst Evol Microbiol 51 : 2145–2165. [Google Scholar]
  2. Bakken JS, Dumler JS, Chen S-M, Eckman MR, Van Etta LL, Walker DH, 1994. Human granulocytic ehrlichiosis in the upper midwest United States, a new species emerging? JAMA 272 : 212–218. [Google Scholar]
  3. Chen S-M, Dumler JS, Bakken JS, Walker DH, 1994. Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human diseases. J Clin Microbiol 32 : 589–595. [Google Scholar]
  4. Walker DH, Dumler JS, 1996. Emergence of the ehrlichioses as human health problems. Emerg Infect Dis 2 : 18–29. [Google Scholar]
  5. Bakken JS, Krueth J, Wilson-Nordskog C, Tilden RL, Asanovich K, Dumler JS, 1996. Clinical and laboratory characteristics of human granulocytic ehrlichiosis. JAMA 275 : 199–205. [Google Scholar]
  6. Bown KJ, Begon M, Bennett M, Woldehiwet Z, Ogden NH, 2003. Seasonal dynamics of Anaplasma phagocytophila in a rodenttick (Ixodes trianguliceps) system, United Kingdom. Emerg Infect Dis 9 : 63–70. [Google Scholar]
  7. Liz JS, Anderes L, Sumner JW, Massung RF, Gern L, Rutti B, Brossard M, 2000. PCR detection of granulocytic ehrlichiae in Ixodes ricinus ticks and wild small mammals in western Switzerland. J Clin Microbiol 38 : 1002–1007. [Google Scholar]
  8. Pancholi P, Kolbert CP, Mitchell PD, Reed KD Jr, Dumler JS, Bakken JS, Telford SR, Persing DH, 1995. Ixodes dammini as a potential vector of human granulocytic ehrlichiosis. J Infect Dis 172 : 1007–1012. [Google Scholar]
  9. Polin H, Hufnagl P, Haunschmid R, Gruber F, Ladurner G, 2004. Molecular evidence of Anaplasma phagocytophilum in Ixodes ricinus ticks and wild animals in Austria. J Clin Microbiol 42 : 2285–2286. [Google Scholar]
  10. Telford SR, Dawson JE, Katavlos P, Warner CK, Kolbert CP, Persing DH, 1996. Perpetuation of the agent of human granulocytic ehrlichiosis in a deer tick-rodent cycle. Proc Natl Acad Sci U S A 93 : 6209–6214. [Google Scholar]
  11. Burkot TR, Maupin GO, Schneider BS, Denatale C, Happ CM, Rutherford JS, 2001. Use of a sentinel host system to study the questing behavior of Ixodes spinipalpis and its role in the transmission of Borrelia bissettii, human granulocytic ehrlichiosis, and Babesia microti. Am J Trop Med Hyg 65 : 293–299. [Google Scholar]
  12. Richter PJ, Kimsey RB, Madigan JE, Barlough JE, Dumler JS, Brooks DL, 1996. Ixodes pacificus (Acari: Ixodidae) as a vector of Ehrlichia equi (Rickettsiales: Ehrlichieae). J Med Entomol 33 : 1–5. [Google Scholar]
  13. Ogden NH, Bown K, Horrocks BK, Woldehiwet Z, Bennett M, 1998. Granulocytic Ehrlichia infection in ixodid ticks and mammals in woodlands and uplands of the U.K. Med Vet Entomol 12 : 423–429. [Google Scholar]
  14. Cinco M, Padovan D, Murgia R, Maroli M, Frusteri L, Heldtander M, 1997. Coexistence of Ehrlichia phagocytophila and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Italy as determined by 16S rRNA gene sequencing. J Clin Microbiol 35 : 3365–3366. [Google Scholar]
  15. Guy E, Tasker S, Joynson DH, 1998. Detection of the agent of human granulocytic ehrlichiosis (HGE) in UK ticks using polymerase chain reaction. Epidemiol Infect 121 : 681–683. [Google Scholar]
  16. Oteo JA, Gil H, Barral M, Pérez A, Jimenez S, Blanco JR, 2001. Presence of granulocytic ehrlichia in ticks and serological evidence of human infection in La Rioja, Spain. Epidemiol Infect 127 : 353–358. [Google Scholar]
  17. Pusterla N, Leutenegger CM, Huder JB, Weber R, Braun U, Lutz H, 1999. Evidence of the human granulocytic ehrlichiosis agent in Ixodes ricinus ticks in Switzerland. J Clin Microbiol 37 : 1332–1334. [Google Scholar]
  18. Schouls LM, Van de Pol I, Rijpkema SGT, Schot CS, 1999. Detection and identification of Ehrlichia, Borrelia burgdorferi sensu lato and Bartonella species in Dutch Ixodes ricinus ticks. J Clin Microbiol 37 : 2215–2222. [Google Scholar]
  19. Sarih M, Ghirbi YM, Bouattour A, Gern L, Baranton G, Postic D, 2005. Detection and identification of Ehrlichia spp. in ticks collected in Tunisia and Morocco. J Clin Microbiol 43 : 1127–1132. [Google Scholar]
  20. Alekseev AN, Dubinina HV, Van De Pol I, Schouls LM, 2001. Identification of Ehrlichia spp. and Borrelia burgdorferi in Ixodes ticks in the Baltic regions of Russia. J Clin Microbiol 39 : 2237–2242. [Google Scholar]
  21. Cao WC, Zhao QM, Zhang PH, Dumler JS, Zhang XT, Fang LQ, Yang H, 2000. Granulocytic Ehrlichiae in Ixodes persulcatus ticks from an area in China where Lyme disease is endemic. J Clin Microbiol 38 : 4208–4210. [Google Scholar]
  22. Cao WC, Zhao QM, Zhang PH, Yang H, Wu XM, Wen BH, Zhang XT, Habbema JDF, 2003. Prevalence of Anplasma phagocytophila and Borrelia burgdorferi in Ixodes persulcatus ticks from northeastern China. Am J Trop Med Hyg 68 : 547–550. [Google Scholar]
  23. Kim CM, Kim MS, Park MS, Park JH, Chae JS, 2003. Identification of Ehrlichia chaffeensis, Anaplasma phagocytophilum, and A. bovis in Haemaphysalis longicornis and Ixodes persulcatus ticks from Korea. Vector Borne Zoonotic Dis 3 : 17–26. [Google Scholar]
  24. Ohashi N, Inayoshi M, Kitamura K, Kawamori F, Kawaguchi D, Nishimura Y, Naitou H, Hiroi M, Masuzawa T, 2005. Anaplasma phagocytophilum–infected ticks, Japan. Emerg Infect Dis 11 : 1780–1783. [Google Scholar]
  25. Inokuma H, Brouqui P, Drancourt M, Raoult D, 2001. Citrate synthase gene sequence: a new tool for phylogenetic analysis and identification of Ehrlichia. J Clin Microbiol 39 : 3031–3039. [Google Scholar]
  26. Wen B, Jian R, Zhang Y, Chen R, 2002. Simultaneous detection of Anaplasma marginale and a new Ehrlichia species closely related to Ehrlichia chaffeensis by sequence analyses of 16S ribosomal DNA in Boophilus microplus ticks from Tibet. J Clin Microbiol 40 : 3286–3290. [Google Scholar]
  27. Retief JD, 2000. Phylogenetic analysis using PHYLIP. Methods Mol Biol 132 : 243–258. [Google Scholar]
  28. Takada N, Fubito I, Fajita H, Wang H-P, Wang J-C, Masuzawa T, 1998. Lyme disease spirochetes in ticks from northeastern China. J Parasitol 84 : 499–504. [Google Scholar]
  29. Barlough JE, Madigan JE, Kramer VL, Clover JR, Hui LT, Webb JP, Vredevoe LK, 1997. Ehrlichia phagocytophila geno-group rickettsiae in ixodid ticks from California collected in 1995 and 1996. J Clin Microbiol 35 : 2018–2021. [Google Scholar]
  30. Von Stedingk LV, Gürtelschmid M, Hanson HS, Gustafson R, Dotevall L, Engvall EO, Granström M, 1997. The human granulocytic ehrlichiosis (HGE) agent in Swedish ticks. Clin Microbiol Infect 3 : 573–574. [Google Scholar]
  31. Sixl W, Petrovec M, Marth E, Wust G, Stunzner D, Schweiger R, 2003. Investigation of Anaplasma phagocytophila infections in Ixodes ricinus and Dermacentor reticulatus ticks in Austria. Ann N Y Acad Sci 990 : 94–97. [Google Scholar]
  32. Holden K, Boothby JT, Anand S, Massung RF, 2003. Detection of Borrelia burgdorferi, Ehrlichia chaffeensis, and Anaplasma phagocytophilum in ticks (Acari: Ixodidae) from a coastal region of California. J Med Entomol 40 : 534–539. [Google Scholar]
  33. Goethert HK, Telford SR 3rd, 2003. Enzootic transmission of the agent of human granulocytic ehrlichiosis among cottontail rabbits. Am J Trop Med Hyg 68 : 633–637. [Google Scholar]
  34. Walls JJ, Greig B, Neitzel DF, Dumler JS, 1997. Natural infection of small mammal species in Minnesota with the agent of human granulocytic ehrlichiosis. J Clin Microbiol 35 : 853–855. [Google Scholar]
  35. Yeh MT, Mather TN, Coughlin RT, Gingrich-Baker C, Sumner JW, Massung RF, 1997. Serologic and molecular detection of granulocytic ehrlichiosis in Rhode Island. J Clin Microbiol 35 : 944–947. [Google Scholar]
  36. Nicholson WL, Muir S, Sumner JW, Childs JE, 1998. Serologic evidence of infection with Ehrlichia spp. in wild rodents (Muridae: Sigmodontinae) in the United States. J Clin Microbiol 36 : 695–700. [Google Scholar]
  37. Foley JE, Lerche NW, Dumler JS, Madigan JE, 1999. A simian model of human granulocytic ehrlichiosis. Am J Trop Med Hyg 60 : 987–993. [Google Scholar]
  38. Bunnell JE, Trigiani ER, Srinivas SR, Dumler JS, 1999. Development and distribution of pathologic lesions are related to immune status and tissue deposition of human granulocytic ehrlichiosis agent-infected cells in a murine model system. J Infect Dis 180 : 546–550. [Google Scholar]

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  • Received : 01 Feb 2006
  • Accepted : 16 May 2006

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