1921
Volume 75, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Malaria transmission from humans to mosquitoes is modulated by human host immune factors. Understanding mechanisms by which the human host response may impair parasite infectivity for mosquitoes has direct implications for the development of transmission-blocking vaccines. We hypothesized that despite a low transmission intensity of malaria in the Peruvian Amazon region of Iquitos, transmission-blocking immunity against might be common, given an unexpectedly high proportion of asymptomatic parasitemic individuals in this region. To test this hypothesis, the ability of symptomatic malaria patients to experimentally infect wild-caught outbred mosquitoes was tested using the indirect membrane feeding technique. Only half (52/102) of parasitemic patients successfully infected mosquitoes. Transmitters were more likely to have gametocytes (OR 6.35, = 0.003), high parasitemia (OR 3.79, = 0.024), and, in terms of basic clinical parameters, a slower pulse rate (mean ± SD: 82.3 ± 12.3 versus 88.7 ± 13.5, = 0.016) than non-transmitters. Log gametocytemia and log real-time reverse transcriptase PCR quantifying gametocytes were significantly and positively correlated with oocyst counts (correlation coefficient 0.505, = 0.26, = 0.001). These experiments are the first to establish a system of determining transmission patterns in experimental infection of outbred natural neotropical malaria vectors in the Amazon region. Patients with inefficiently infect outbred mosquitoes, raising the possibility that some degree of naturally occurring transmission-blocking immunity is present on a population basis in the Peruvian Amazon, an area of low intensity of malaria transmission.

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2006-10-01
2017-09-22
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  • Received : 17 Mar 2006
  • Accepted : 15 Jun 2006

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