1921
Volume 75, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

O157:H7 producing Shiga like toxins is a food-borne pathogen frequently isolated in Bangui from patients with hemorrhagic colitis (HC). This survey provides comprehensive data on the high prevalence of O157:H7 infection in Bangui: carriage of O157:H7 by zebu () and fish, contamination of the fields at N’Goila where the butchers kill the zebus, and contamination of the field surface water along the M’Poko River upstream of the Oubangui River where fish are caught, appear to be important contributory factors. We also describe novel strains of serogroup O157:NM isolated from zebu and from fish; a variety of assays indicate that these strains belong to the enteropathogenic pathotype, though they lack certain genetic elements thought to be diagnostic for this pathotype.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.2006.75.513
2006-09-01
2017-09-24
Loading full text...

Full text loading...

/deliver/fulltext/14761645/75/3/0750513.html?itemId=/content/journals/10.4269/ajtmh.2006.75.513&mimeType=html&fmt=ahah

References

  1. Germani Y, Soro B, Vohito MD, Morel O, Morvan J, 1997. Enterohaemorrhagic Escherichia coli in Central African Republic. Lancet 349 : 1670.
  2. Greatorex JS, Thorne GM, 1994. Humoral immune responses to Shiga-like toxins and Escherichia coli O157 lipopolysaccharide in hemolyticuremic syndrome patients and healthy subjects. J Clin Microbiol 32 : 1172–1178.
  3. Sanath KH, Otta SK, Karunasagar I, Karunasagar I, 2001. Detection of Shiga-toxigenic Escherichia coli (STEC) in fresh seafood and meat marketed in Mangalore, India by PCR. Lett Appl Microbiol 33 : 334–338.
  4. Pollard DR, Johnson WM, Lior H, Tyler SD, Rozee R, 1990. Rapid and specific detection of verotoxin genes in Escherichia coli by the polymerase chain reaction. J Clin Microbiol 28 : 540–545.
  5. Pollard DR, Johnson WM, Lior H, Tyler SD, Rozee R, 1991. Identification of verotoxin 2 variant B subunit genes in Escherichia coli by polymerase chain reaction and restriction fragment length polymorphism analysis. J Clin Microbiol 29 : 1339–1343.
  6. Beaudry M, Zhu C, Fairbrother JM, Harel J, 1996. Genotypic and phenotypic characterization of Escherichia coli isolates from dogs manifesting attaching and effacing lesions. J Clin Microbiol 34 : 144–148.
  7. Gunzburg ST, Tornieporth NG, Riley LW, 1995. Identification of enteropathogenic Escherichia coli by PCR-based detection of the bundle-forming pilus gene. J Clin Microbiol 33 : 1375–1377.
  8. Germani Y, Begaud E, Desperrier JM, 1994. Easy to perform modified Elek test to identify Shiga like toxin producing diarrheagenic Escherichia coli. Res Microbiol 145 : 333–340.
  9. Welinder-Olsson C, Kjellin E, Badenfors M, Kaijser B, 2000. Improved microbiological techniques using the polymerase chain reaction and pulsed-field gel electrophoresis for diagnosis and follow-up of enterohaemorrhagic Escherichia coli infection. Eur J Clin Microbiol Infect Dis 19 : 843–851.
  10. Gautom RK, 1997. Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day. J Clin Microbiol 35 : 2977–2980.
  11. Fields PI, Blom K, Hughes HJ, Helsel LO, Feng P, Swaminathan B, 1997. Molecular characterization of the gene encoding H antigen in Escherichia coli and development of a PCR-restriction fragment length polymorphism test for identification of E. coli O157:H7 and O157:NM. J Clin Microbiol 35 : 1066–1070.
  12. Feng P, Lampel KA, 1994. Genetic analysis of uidA expression in enterohaemorrhagic Escherichia coli serotype O157:H7. Microbiol 140 : 2101–2107.
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2006.75.513
Loading
  • Received : 14 Sep 2005
  • Accepted : 06 Apr 2006

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error