Volume 75, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Outbreaks of bovine vaccinia disease caused by circulation of (VACV) strains have been a common occurrence in Brazil in the recent years, being an important emergent zoonosis. During a single outbreak that took place in 2001, two genetically different VACV strains were isolated and named Guarani P1 virus (GP1V) and Guarani P2 virus (GP2V). Molecular diagnosis was done through restriction fragment length polymorphism (RFLP) of gene (A26L) and by sequence analysis of a group of five VACV genes including the , , , , and genes. These findings confirmed the co-circulation of two different strains during the same outbreak, raising important questions about the origin, emergence, and circulation of VACV strains in Brazil.


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  • Received : 15 Feb 2006
  • Accepted : 10 May 2006

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