1921
Volume 75, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Serologic surveys in Egypt have documented human and animal exposure to vector-borne bacterial pathogens, but the presence and distribution of these agents in arthropods has not been determined. Between July 2002 and July 2003, fleas were collected from 221 mammals trapped in 17 cities throughout Egypt. A total of 987 fleas were collected, representing four species (, , , and ); 899 of these fleas were from rats ( spp.). Fleas were tested for DNA from spp., spp., , spp., spp., and , the agent of murine typhus, was detected in and from rats from nine cities. A spotted-fever group sp. similar to “RF2125” was detected in , and two unidentified spotted fever group were detected in two . Novel genotypes were detected in and from three cities. was detected in two fleas. , , and were not detected.

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References

  1. Khan IA, 2004. Plague: the dreadful visitation occupying the human mind for centuries. Trans R Soc Trop Med Hyg 98 : 270–277. [Google Scholar]
  2. Hussein AG, 1955. Changes in the epidemiology of plague in Egypt, 1899–1951. Bull World Health Organ 13 : 27–48. [Google Scholar]
  3. Moe JB, Pedersen CE Jr, 1980. The impact of rickettsial diseases on military operations. Mil Med 145 : 780–785. [Google Scholar]
  4. Kaplan MM, Bertagna P, 1955. The geographical distribution of Q fever. Bull World Health Organ 13 : 829–860. [Google Scholar]
  5. Taylor RM, Kingston JR, Rizk F, 1957. A note on typhus in Egypt and the Sudan. Am J Trop Med Hyg 6 : 863–870. [Google Scholar]
  6. Mooser H, Imam IZE, Abbas M, Morcos EG, Abbas M, 1963. Une enquête sérologique sur le typhus en Égypte. Bull Soc Pathol Exot 56 : 586–589. [Google Scholar]
  7. Botros BA, Elmolla MS, Salib AW, Calamaio CA, Dasch GA, Arthur RR, 1995. Canine ehrlichiosis in Egypt: seroepidemiological survey. Onderstepoort J Vet Res 62 : 41–43. [Google Scholar]
  8. Corwin A, Habib M, Watts D, Darwish M, Olson J, Botros B, Hibbs R, Kleinosky M, Lee HW, Shope R, Kilpatrick M, 1993. Community-based prevalence profile of arboviral, rickettsial, and Hantaan-like viral antibody in the Nile River Delta of Egypt. Am J Trop Med Hyg 48 : 776–783. [Google Scholar]
  9. Corwin A, Habib M, Olson J, Scott D, Ksiazek T, Watts DM, 1992. The prevalence of arboviral, rickettsial, and Hantaan-like viral antibody among schoolchildren in the Nile river delta of Egypt. Trans R Soc Trop Med Hyg 86 : 677–679. [Google Scholar]
  10. Botros BA, Soliman AK, Darwish M, el Said S, Morrill JC, Ksiazek TG, 1989. Seroprevalence of murine typhus and fievre boutonneuse in certain human populations in Egypt. J Trop Med Hyg 92 : 373–378. [Google Scholar]
  11. Corwin A, Habib M, Olson J, Scott D, Ksiazek T, Watts DM, 1992. The prevalence of arboviral, rickettsial, and Hantaan-like viral antibody among schoolchildren in the Nile river delta of Egypt. Trans R Soc Trop Med Hyg 86 : 677–679. [Google Scholar]
  12. Hoogstraal H, Kaiser MN, Ormsbee RA, Osborn DJ, Helmy I, Gaber S, 1967. Hyalomma (Hyalommina) rhipicephaloides Neumann (Ixodoidea: Ixodidae): its identity, hosts, and ecology, and Rickettsia conori, R. prowazeki, and Coxiella burneti infections in rodent hosts in Egypt. J Med Entomol 4 : 391–400. [Google Scholar]
  13. Ormsbee RA, Hoogstraal H, Yousser LB, Hildebrandt P, Atalla W, 1968. Evidence for extra-human epidemic typhus in the wild animals of Egypt. J Hyg Epidemiol Microbiol Immunol 12 : 1–6. [Google Scholar]
  14. Shoukry A, Merdan AI, El Kady GA, 1991. The role of rodents as reservoirs for some diseases in new reclaimed areas in north Sinai. J Egypt Soc Parasitol 21 : 513–519. [Google Scholar]
  15. Soliman AK, Botros BA, Ksiazek TG, Hoogstraal H, Helmy I, Morrill JC, 1989. Seroprevalence of Rickettsia typhi and Rickettsia conorii infection among rodents and dogs in Egypt. J Trop Med Hyg 92 : 345–349. [Google Scholar]
  16. Ali AM, Salit AM, Sheta I, Mourad GM, 1974. Ectoparasites on rodents in the Nile Valley, upper Egypt. J Egypt Public Health Assoc 49 : 3–19. [Google Scholar]
  17. Iman IZE, Alfy L, 1966. Evidence of typhus infection in domestic animals in Egypt. Bull World Health Organ 35 : 123–126. [Google Scholar]
  18. Childs JE, Olson JG, Wolf A, Cohen N, Fakile Y, Rooney JA, Bacellar F, Regnery RL, 1995. Prevalence of antibodies to Rochalimaea species (cat-scratch disease agent) in cats. Vet Rec 136 : 519–520. [Google Scholar]
  19. Bakr ME, Morsy TA, Nassef NE, El Meligi MA, 1996. Flea ectoparasites of commensal rodents in Shebin El Kom, Menoufia Governorate, Egypt. J Egypt Soc Parasitol 26 : 39–52. [Google Scholar]
  20. Khalid MLM, Morsy TA, el Shennawy SFA, Farrag AMMK, Sabry AHA, Mostafa HAA, 1992. Studies on flea fauna in El Fayoum Governorate, Egypt. J Egypt Soc Parasitol 22 : 783–799. [Google Scholar]
  21. Morsy TA, Fayad ME, Abou Shady MK, Yousef NS, 1986. Ectoparasites of rodents in Suez governorate with special reference to fleas. J Egypt Soc Parasitol 16 : 457–468. [Google Scholar]
  22. Shoukry A, Morsy TA, Abu Hashish TA, El Kady GA, 1986. Seasonal activities of two commensal rats and flea index in North Sinai governorate, Egypt. J Egypt Soc Parasitol 16 : 385–393. [Google Scholar]
  23. Shoukry A, Morsy TA, Farahat AA, 1987. Arthropod-ectoparasites of rodents trapped in Ismailia Governorate, Egypt. J Egypt Soc Parasitol 17 : 525–537. [Google Scholar]
  24. Soliman S, Main AJ, Marzouk AS, Montasser AA, 2001. Seasonal studies on commensal rats and their ectoparasites in a rural area of Egypt: the relationship of ectoparasites to the species, locality, and relative abundance of the host. J Parasitol 87 : 545–553. [Google Scholar]
  25. Morsy TA, Michael SA, Bassili WR, Saleh MS, 1982. Studies on rodents and their zoonotic parasites, particularly leishmania, in Ismailiya Governorate, A.R. Egypt. J Egypt Soc Parasitol 12 : 565–585. [Google Scholar]
  26. Morsy TA, el Ela RGA, el Hariry MA, 1988. The commensal rodents and their flea fauna in Alexandria City, Egypt. J Egypt Soc Parasitol 18 : 11–28. [Google Scholar]
  27. Azad AF, Traub R, 1987. Transmission of murine typhus rickettsiae by Leptopsylla segnis (Siphonaptera: Leptopsyllidae). J Med Entomol 24 : 689–693. [Google Scholar]
  28. Strandtmann RW, Eben DJ, 2005. A survey of typhus in rats and rat ectoparasites in Galveston, Texas. Tex Rep Biol Med 11 : 144–151. [Google Scholar]
  29. Morsy TA, Michael SA, Bassili WR, Saleh MS, 1982. Studies on rodents and their zoonotic parasites, particularly leishmania, in Ismailiya Governorate, A.R. Egypt. J Egypt Soc Parasitol 12 : 565–585. [Google Scholar]
  30. Osborn DJ, Helmy I, 1980. The contemporary land mammals of Egypt (including Sinai). Fieldiana Zool 5 : 1–579. [Google Scholar]
  31. Lewis RE, 1967. The fleas (Siphonaptera) of Egypt. An illustrated and annotated key. J Parasitol 53 : 863–885. [Google Scholar]
  32. Hoogstraal H, Traub R, 1965. The fleas (Siphonaptera) of Egypt. Host-parasite relationships of rodents of the families Spalacidae, Muridae, Gliridae, Dipodidae, and Hystricidae. J Egypt Public Health Assoc 40 : 343–379. [Google Scholar]
  33. Moriarity JR, Loftis AD, Dasch GA, 2005. High-throughput molecular testing of ticks using a liquid-handling robot. J Med Entomol 42 : 1063–1067. [Google Scholar]
  34. Li JS, Chu F, Reilly A, Winslow GM, 2002. Antibodies highly effective in SCID mice during infection by the intracellular bacterium Ehrlichia chaffeensis are of picomolar affinity and exhibit preferential epitope and isotype utilization. J Immunol 169 : 1419–1425. [Google Scholar]
  35. Jiang J, Chan TC, Temenak JJ, Dasch GA, Ching WM, Richards AL, 2004. Development of a quantitative real-time polymerase chain reaction assay specific for Orientia tsutsugamushi. Am J Trop Med Hyg 70 : 351–356. [Google Scholar]
  36. Tomaso H, Reisinger EC, Al Dahouk S, Frangoulidis D, Rakin A, Landt O, Neubauer H, 2003. Rapid detection of Yersinia pestis with multiplex real-time PCR assays using fluorescent hybridisation probes. FEMS Immunol Med Microbiol 38 : 117–126. [Google Scholar]
  37. Roux V, Raoult D, 1995. The 16S–23S rRNA intergenic spacer region of Bartonella (Rochalimaea) species is longer than usually described in other bacteria. Gene 156 : 107–111. [Google Scholar]
  38. Zeaiter Z, Fournier PE, Ogata H, Raoult D, 2002. Phylogenetic classification of Bartonella species by comparing groEL sequences. Int J Syst Evol Microbiol 52 : 165–171. [Google Scholar]
  39. Stein A, Raoult D, 1992. Detection of Coxiella burnetii by DNA amplification using polymerase chain reaction. J Clin Microbiol 30 : 2462–2466. [Google Scholar]
  40. Carl M, Tibbs CW, Dobson ME, Paparello S, Dasch GA, 1990. Diagnosis of acute typhus infection using the polymerase chain reaction. J Infect Dis 161 : 791–793. [Google Scholar]
  41. Tzianabos T, Anderson BE, McDade JE, 1989. Detection of Rickettsia rickettsii DNA in clinical specimens by using polymerase chain reaction technology. J Clin Microbiol 27 : 2866–2868. [Google Scholar]
  42. Roux V, Rydkina E, Eremeeva M, Raoult D, 1997. Citrate synthase gene comparison, a new tool for phylogenetic analysis, and its application for the rickettsiae. Int J Syst Bacteriol 47 : 252–261. [Google Scholar]
  43. Stevenson HL, Bai Y, Kosoy MY, Montenieri JA, Lowell JL, Chu MC, Gage KL, 2003. Detection of novel Bartonella strains and Yersinia pestis in prairie dogs and their fleas (Siphonaptera: Ceratophyllidae and Pulicidae) using multiplex polymerase chain reaction. J Med Entomol 40 : 329–337. [Google Scholar]
  44. Azad AF, Sacci JB Jr, Nelson WM, Dasch GA, Schmidtmann ET, Carl M, 1992. Genetic characterization and transovarial transmission of a typhus-like rickettsia found in cat fleas. Proc Natl Acad Sci USA 89 : 43–46. [Google Scholar]
  45. National Center for Biotechnology Information, 2004. BLAST. [2.2.10]. Bethesda, MD: National Library of Medicine, National Institutes of Health.
  46. Thompson JD, Higgins DG, Gibson TJ, 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22 : 4673–4680. [Google Scholar]
  47. Felsenstein J, 1989. PHYLIP—phylogeny inference package (version 3.2). Cladistics 5 : 164–166. [Google Scholar]
  48. Loftis AD, Massung RF, Levin ML, 2003. Quantitative real-time PCR assay for detection of Ehrlichia chaffeensis. J Clin Microbiol 41 : 3870–3872. [Google Scholar]
  49. Parola P, Sanogo OY, Lerdthusnee K, Zeaiter Z, Chauvancy G, Gonzalez JP, Miller RS, Telford SR III, Wongsrichanalai C, Raoult D, 2003. Identification of Rickettsia spp. and Bartonella spp. in fleas from the Thai-Myanmar border. Ann N Y Acad Sci 990 : 173–181. [Google Scholar]
  50. Reeves WK, Nelder MP, Korecki JA, 2005. Bartonella and Rickettsia in fleas and lice from mammals in South Carolina, USA. J Vector Ecol 30 , 310–315. [Google Scholar]
  51. Morsy TA, Michael SA, Bassili WR, Saleh MS, 1982. Studies on rodents and their zoonotic parasites, particularly leishmania, in Ismailiya Governorate, A.R. Egypt. J Egypt Soc Parasitol 12 : 565–585. [Google Scholar]
  52. McQuiston JH, Childs JE, 2002. Q fever in humans and animals in the United States. Vector Borne and Zoonotic Diseases 2 : 179–191. [Google Scholar]
  53. Paddock CD, Childs JE, 2003. Ehrlichia chaffeensis: a prototypical emerging pathogen. Clin Microbiol Rev 16 : 37–64. [Google Scholar]
  54. Bakken JS, Dumler JS, 2000. Human granulocytic ehrlichiosis. Clin Infect Dis 31 : 554–560. [Google Scholar]
  55. Blanco JR, Oteo JA, 2002. Human granulocytic ehrlichiosis in Europe. Clin Microbiol Infect 8 : 763–772. [Google Scholar]
  56. Darwish MA, Feinsod FM, Scott RM, Ksiazek TG, Botros BA, Farrag IH, el Said S, 1987. Arboviral causes of non-specific fever and myalgia in a fever hospital patient population in Cairo, Egypt. Trans R Soc Trop Med Hyg 81 : 1001–1003. [Google Scholar]
  57. Dobson ME, Azad AF, Dasch GA, Webb L, Olson JG, 1989. Detection of murine typhus infected fleas with an enzyme-linked immunosorbent assay. Am J Trop Med Hyg 40 : 521–528. [Google Scholar]
  58. Wedincamp J Jr, Foil LD, 2002. Vertical transmission of Rickettsia felis in the cat flea (Ctenocephalides felis Bouche). J Vector Ecol 27 : 96–101. [Google Scholar]
  59. Haas GE, Tomich Q, 1973. The fleas (Siphonaptera) of Egypt. Early records from mammals. J Egypt Public Health Assoc 48 : 1–17. [Google Scholar]
  60. Hoogstraal H, Traub R, 1966. The fleas (Siphonaptera) of Egypt. Host-parasite relationships of carnivora. J Egypt Public Health Assoc 41 : 75–91. [Google Scholar]
  61. Mullen G, Durden L, 2002. Medical and Veterinary Entomology. San Diego, CA: Academic Press.
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  • Received : 12 Sep 2005
  • Accepted : 21 Feb 2006

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