Volume 75, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


During the outbreak of highly pathogenic avian influenza that occurred in Tamba Town, Kyoto Prefecture in 2004, a total of 926 flies were collected from six sites within a radius of 2.3 km from the poultry farm. The H5 influenza A virus genes were detected from the intestinal organs, crop, and gut of the two blow fly species, and , by reverse transcription-polymerase chain reaction for the matrix protein () and hemagglutinin () genes. The gene encoding multiple basic amino acids at the cleavage site indicated that this virus is a highly pathogenic strain. Based on the full-length sequences of the and neuraminidase () segments of virus isolates through embryonated chicken eggs, the virus from (A/blow fly/Kyoto/93/2004) was characterized as H5N1 subtype influenza A virus and shown to have > 99.9% identities in all three RNA segments to a strain from chickens (A/chicken/Kyoto/3/2004) and crows (A/crows/Kyoto/53/2004) derived during this outbreak period in Kyoto in 2004. Our results suggest it is possible that blow flies could become a mechanical transmitter of H5N1 influenza virus.


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  • Received : 29 Aug 2005
  • Accepted : 25 Apr 2006

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