Volume 75, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Using ITS2 gene sequences, the validity of the tapeworm (Nybelin, 1931), infecting humans on the Pacific coast of South America and in Japan, was assessed. ITS2 sequences of this cestode differed markedly (sequence similarity 79.0–80.2%) from those of the most common human-infecting cestode, the broad fish tapeworm (L.), as well as other four species of , including potential human parasites (, and ) and two species of (sequence similarity 77.5–81.9%). Interspecific sequence similarity between all but one () species was 86.1–99.6%, whereas individual isolates of and exhibited intraspecific sequence similarity of 97.0–98.0% and 98.2–99.9%, respectively. Phylogenetic trees constructed from ITS2 sequences show a markedly distant position of from other species analyzed and also indicate the possible paraphyly of .


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  1. Muller R, 2002. Worms and Human Disease. Second edition. Wallingford: CABI Publishing.
  2. Chai J-Y, Murrell KD, Lymbery AJ, 2005. Fish-borne parasitic zoonoses: Status and issues. Int J Parasitol 35 : 1233–1254.
    [Google Scholar]
  3. Williams HH, Jones A, 1994. Parasitic Worms of Fish. London: Taylor & Francis.
  4. Nybelin O, 1931. Säugertier und Vogelcestoden von Juan Fernandez. Skottsberg C, ed. Natural History of Juan Fernandez und Easter Island. Volume 3. 493–523.
  5. Baer JG, 1969. Diphyllobothrium pacificum, a tapeworm from sea lions endemic in man along the coastal area of Peru. J Fish Res Board Can 26 : 717–723.
    [Google Scholar]
  6. Kamo H, Maejima J, Yazaki S, 1982. Occurrence of human infection with Diphyllobothrium pacificum (Nybelin, 1931) Margolis, 1956 in Japan. Jap J Parasitol 31 : 165–170.
    [Google Scholar]
  7. Tsuboi T, Torii M, Hirai K, 1993. Light and scanning electron microscopy of Diphyllobothrium pacificum expelled from a man. Jap J Parasitol 42 : 422–428.
    [Google Scholar]
  8. Yamaguti S, 1951. Studies on the helminth fauna of Japan. Part 47. Cestodes of marine mammals and birds. Jap J Zool 12: 307–314 +2 plates.
  9. Torres P, Franjola R, Figueroa L, Schlatter R, González H, Contreras B, Martín R, 1981. Researches on Pseudophyllidea (Carus, 1813) in the south of Chile. IV Occurrence of Diphyllobothrium dendriticum (Nitszch). J Helminthol 55 : 173–187.
    [Google Scholar]
  10. Torres P, Franjola R, Pérez J, Auad S, Uherek F, Miranda JC, Flores L, Riquelme J, Salazar S, Hermosilla C, Rojo R, 1989a. Epidemiología de la difilobotriasis en la cuenca del río Valdivia, Chile. Rev Saúde Pública São Paulo 23 : 45–57.
    [Google Scholar]
  11. Torres P, Torres J, Garrido O, Thibaut J, 1989b. Investigaciones sobre Pseudophyllidea (Carus, 1813) en el sur de Chile. X. Observaciones experimentales sobre la coexistencia de plerocercoides de Diphyllobothrium latum (L.) y Diphyllobothrium dendriticum (Nitszch) en salmónidos de la cuenca del río Valdivia. Arch Med Vet 21 : 51–57.
    [Google Scholar]
  12. Torres P, Gesche W, Montefusco A, Miranda JC, Dietz P, Huijse R, 1998. Diphyllobothriosis humana y en peces del lago Riñihue, Chile: Efecto de la actividad educativa, distribución estacional y relación con sexo, talla y dieta de los peces. Arch Med Vet 30 : 31–45.
    [Google Scholar]
  13. Torres P, López JC, Cubillos V, Lobos C, Silva R, 2002. Visceral diphyllobothriosis in a cultured rainbow trout, Oncorhynchus mykiss (Walbaum), in Chile. J Fish Dis 25 : 375–379.
    [Google Scholar]
  14. Torres P, Cuevas C, Tang M, Barra M, Franjola R, Navarrete N, Montefusco A, Otth L, Wilson G, Puga S, Figueroa L, Cerda O, 2004. Introduced and native fishes as infection foci of Diphyllobothrium spp. in humans and dogs from two localities at Lake Panguipulli in Southern Chile. Comp Parasitol 71 : 111–117.
    [Google Scholar]
  15. Santos FLN, de Faro LB, 2005. The first confirmed case of Diphyllobothrium latum in Brazil. Mem Inst Oswaldo Cruz 100 : 585–586.
    [Google Scholar]
  16. Yamaguti S, 1959. Systema Helminthum, Volume 2. The Cestodes of Vertebrates. New York: Interscience Publishers.
  17. Scholz T, Škeříková A, Hanzelová V, Koubková B, Baruš V, 2003. Resurrection of Proteocephalus sagittus (Grimm, 1872) (Cestoda: Proteocephalidea) based on morphological and molecular data. Syst Parasitol 56 : 173–181.
    [Google Scholar]
  18. Hall TA, 1999. Bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41 : 95–98.
    [Google Scholar]
  19. Swofford DL, 1998. PAUP 4.0-Phylogenetic Analysis Using Parsimony. Version 4. Sunderland, MA: Sinauer Associates.
  20. Zhu XQ, Beveridge I, Berger L, Barton D, Gasser RB, 2002. Single-strand conformation polymorphism-based analysis reveals genetic variation within Spirometra erinacei (Cestoda: Pseudophyllidea) from Australia. Mol Cell Probes 16 : 159–165.
    [Google Scholar]
  21. Mueller JF, 1937. A repartition of the genus Diphyllobothrium. J Parasitol 23 : 308–310.
    [Google Scholar]
  22. Schmidt GD, 1986. CRC Handbook of Tapeworm Identification.Boca Raton, FL: CRC Press.
  23. Bray RA, Jones A, Andersen KI, 1994. Order Pseudophyllidea Carus, 1863. Khalil LF, Jones A, Bray RA, eds. Keys to the Cestode Parasites of Vertebrates. Wallingford, UK: CAB International, 205–247.

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  • Received : 14 Feb 2006
  • Accepted : 05 Apr 2006
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