Volume 75, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Seventeen stocks isolated from cutaneous lesions of Pakistani patients were studied by multilocus enzyme electrophoresis and by polymerase chain reaction amplification and sequencing of the cytochrome ( b) gene. Eleven stocks that expressed nine zymodemes were assigned to . All of them were isolated from patients in the lowlands of Larkana district and Sibi city in Sindh and Balochistan provinces, respectively. The remaining six, distributed in two zymodemes (five and one), isolated from the highland of Quetta city, Balochistan, were identified as . The same result at species level was obtained by the b sequencing for all the stocks examined. No clear-cut association between the clinical features (wet or dry type lesions) and the species involved was found. was highly polymorphic compared with . This difference may be explained by the fact that humans may act as a sole reservoir of in anthroponotic cycles; however, many wild mammals can be reservoirs of in zoonotic cycles.


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  • Received : 20 Oct 2005
  • Accepted : 09 Mar 2006

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