1921
Volume 75, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Recently, two techniques, polymerase chain reaction (PCR) amplification and sequencing of cytochrome gene ( b gene sequencing) and polymorphism-specific PCR (PS-PCR) were recommended for species identification. Before this study, however, the accuracy of these methods had not been tested against the multilocus enzyme electrophoresis, the current gold standard technique on this task. Therefore, a trial was done for the first time to compare the results obtained by these techniques, using 17 Argentinean stocks in independent assays. For all the stocks examined, the same results at species level were obtained by the three techniques. Among them, 14 were assigned to , and three to . The two techniques, b gene sequencing and PS-PCR, were able to distinguish between all the proven species responsible for leishmaniases in Argentina. Thus, both techniques were validated and could be used independently for the species designation of parasites in the country.

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2006-08-01
2017-11-21
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  • Received : 10 Aug 2005
  • Accepted : 20 Apr 2006

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