1921
Volume 75, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Methods for early diagnosis of pediatric HIV-1 infection (DNA-polymerase chain reaction [PCR], RNA quantification, viral cultures) are expensive. Most Cambodian infants wait 18 months for HIV serologic tests. We observed that boosted-p24-antigen profile assay, with performances similar to viral cultures and costs similar to DNA-PCR, is easier to perform and could readily be set up in resource-poor settings.

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2006-12-01
2017-11-17
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References

  1. UNAIDS, 2002. Report on the Global HIV/AIDS Epidemic. Geneva, Switzerland.
  2. Delfraissy JF, 2002. Diagnostic de l’Infection chez le Nouveau-né et l’Enfant. Rapport 2002. Sous la Direction du Professeur J. F. Delfraissy. Prise en Charge des Personnes Infectées par le VIH. Recommandation du Groupe d’Experts. Paris: Médecine-Sciences.
  3. Sherman GG, Stevens G, Stevens WS, 2004. Affordable diagnosis of human immunodeficiency virus infection in infants by p24 antigen detection. Pediatr Infect Dis J 23 : 173–176.
  4. Schupbach J, Boni J, Tomasik Z, Jendis J, Seger R, Kind C, 1994. Sensitive detection and early prognostic significance of p24 antigen in heat-denatured plasma of human immunodeficiency virus type 1-infected infants. Swiss Neonatal HIV Study Group. J Infect Dis 170 : 318–324.
  5. Nadal D, Boni J, Kind C, Varnier OE, Steiner F, Tomasik Z, Schupbach J, 1999. Prospective evaluation of amplification-boosted ELISA for heat-denatured p24 antigen for diagnosis and monitoring of pediatric human immunodeficiency virus type 1 infection. J Infect Dis 180 : 1089–1095.
  6. Lyamuya E, Bredberg-Raden U, Massawe A, Urassa E, Kawo G, Msemo G, Kazimoto T, Ostborn A, Karlsson K, Mhalu F, Biberfeld G, 1996. Performance of a modified HIV-1 p24 antigen assay for early diagnosis of HIV-1 infection in infants and prediction of mother-to-infant transmission of HIV-1 in Dar es Salaam, Tanzania. J Acquir Immune Defic Syndr Hum Retro-virol 12 : 421–426.
  7. Sutthent R, Gaudart N, Chokpaibulkit K, Tanliang N, Kanoksinsombath C, Chaisilwatana P, 2003. p24 Antigen detection assay modified with a booster step for diagnosis and monitoring of human immunodeficiency virus type 1 infection. J Clin Microbiol 41 : 1016–1022.
  8. Schupbach J, 2003. Viral RNA and p24 antigen as markers of HIV disease and antiretroviral treatment success. Int Arch Allergy Immunol 132 : 196–209.
  9. http://www.fda.gov/cdrh/oivd/guidance/1588.html.
  10. Yourno J, Conroy J, 1992. A novel polymerase chain reaction method for detection of human immunodeficiency virus in dried blood spots on filter paper. J Clin Microbiol 30 : 2887–2892.
  11. Fransen K, Zhong P, De Beenhouwer H, Carpels G, Peeters M, Louwagie J, Janssens W, Piot P, van der Groen G, 1994. Design and evaluation of new, highly sensitive and specific primers for polymerase chain reaction detection of HIV-1 infected primary lymphocytes. Mol Cell Probes 8 : 317–322.
  12. Burgard M, Mayaux M, Blanche S, Ferroni A, Guihard-Moscato M, Allemon M, Ciraru-Vigneron N, Firtion G, Floch C, Guillot F, et al., 1992. The use of viral culture and p24 antigen testing to diagnose human immunodeficiency virus infection in neonates. The HIV Infection in Newborns French Collaborative Study Group. N Engl J Med 327 : 1192–1197.
  13. Schupbach J, Boni J, Bisset LR, Tomasik Z, Fischer M, Gunthard HF, Ledergerber B, Opravil M, 2003. HIV-1 p24 antigen is a significant inverse correlate of CD4 T-cell change in patients with suppressed viremia under long-term antiretroviral therapy. J Acquir Immune Defic Syndr 33 : 292–299.
  14. Rouet F, Ekouevi DK, Chaix ML, Burgard M, Inwoley A, Tony TD, Danel C, Anglaret X, Leroy V, Msellati P, Dabis F, Rouzioux C, 2005. Transfer and evaluation of an automated, low-cost real-time reverse transcription-PCR test for diagnosis and monitoring of human immunodeficiency virus type 1 infection in a West African resource-limited setting. J Clin Microbiol 43 : 2709–2717.
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  • Received : 19 Apr 2006
  • Accepted : 19 Aug 2006

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