1921
Volume 74, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

The purpose of this study was to show that individual malaria rapid diagnosis tests (MRDTs) could also be used to isolate DNA for genetic studies. We extracted and amplified Plasmodium DNA using two commercial MRDT kits. Phenol/chloroform extraction followed by a nested polymerse chain reaction (PCR) can be used to identify and from MRDTs. The PCR on MRDT-isolated DNA was more sensitive than antigen capture by MRDT. Satisfactory results were also obtained if older MRDT tests were used, even after long periods of storage at ambient temperature, with no special preservation.

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2006-06-01
2017-11-20
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  • Received : 13 Jun 2005
  • Accepted : 08 Nov 2005

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