1921
Volume 74, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

The alphaviruses o’nyong nyong virus (ONNV) and chikungunya virus (CHIKV) provide a unique system to study the viral genes involved in vector specificity. ONNV infects both anopheline and culicine mosquitoes, whereas CHIKV infects only culicine mosquitoes. In this study, chimeric viruses were constructed that contained genes from both ONNV and CHIKV. These chimeras and previously described full-length infectious clones of ONNV and CHIKV were evaluated in and mosquitoes. Virus derived from the infectious clones of ONNV and CHIKV retained the vector specificity of the parental viruses. All six of the chimeras were found to infect mosquitoes at high rates but only the chimera containing viral genes encoding all of the structural proteins of ONNV was able to infect mosquitoes. These data indicate that all of the viral structural proteins are necessary for ONNV to infect mosquitoes.

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2006-04-01
2017-11-19
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References

  1. Karabatsos N, 1985. International Catalogue of Arboviruses. Third edition. San Antonio, TX: American Society of Tropical Medicine and Hygiene.
  2. Strauss JH, Strauss EG, 1994. The alphaviruses: gene expression, replication, and evolution. Microbiol Rev 58 : 491–562.
  3. Johnson BK, 1988. O’nyong-nyong virus disease. Monath TP, ed. The Arboviruses: Epidemiology and Ecology. Volume II. Boca Raton, FL: CRC Press, 217–223.
  4. Williams MC, Woodall JP, Corbet PS, Gillett JD, 1965. O’nyong-nyong fever: an epidemic virus disease in East Africa. VIII. Virus isolations from Anopheles mosquitoes. Trans R Soc Trop Med Hyg 59 : 300–306.
  5. Williams MC, Woodall JP, 1961. O’nyong nyong fever: an epidemic virus disease in east Africa: isolation and some properties of the virus. Trans R Soc Trop Med Hyg 55 : 135–141.
  6. Lanciotti RS, Ludwig ML, Rwaguma EB, Lutwama JJ, Kram T, Karabatsos N, Cropp BC, Miller BR, 1998. Emergence of O’nyong-nyong fever in Uganda after a 35-year absence: genetic characterization of the virus. Virol 252 : 258–268.
  7. Powers AM, Brault AC, Tesh RB, Weaver SC, 2000. Re-emergence of chikungunya and o’nyong-nyong viruses: evidence for distinct geographical lineages and distant evolutionary relationships. J Gen Virol 81 : 471–479.
  8. Haddow AJ, Davies CW, Walker AJ, 1960. O’nyong nyong fever: an epidemic virus disease in east Africa: introduction. Trans R Soc Trop Med Hyg 54 : 517–522.
  9. Rwaguma E, Lutwama JJ, Sempala SDK, Kiwanuka N, Kamugisha J, Okware S, Bagambisa G, Lanciotti R, Roehrig JT, Gubler DJ, 1997. Emergence of epidemic O’nyong nyong fever in southwestern Uganda after an absence of 35 years (letter). Emerg Infect Dis 3 : 77.
  10. Tesh RB, 1982. Arthritides caused by mosquito-borne viruses. Annu Rev Med 33 : 31–40.
  11. Pavri KM, 1964. Presence of chikungunya antibodies in human sera collected from Calcutta and Jamshedpur before 1963. Indian J Med Res 52 : 698–702.
  12. Jupp PG, McIntosh BM, 1988. Chikungunya virus disease. Monath TP, ed. The Arboviruses: Epidemiology and Ecology. Volume II. Boca Raton, FL: CRC Press, 137–157.
  13. Laras K, Sukri NC, Larasati RP, Bangs MJ, Kosim R, Djauzi, Wandra T, Master J, Kosasih H, Hartati S, Beckett C, Sedyaningsih ER, Beecham HJ III, Corwin AL, 2005. Tracking the re-emergence of epidemic chikungunya virus in Indonesia. Trans R Soc Trop Med Hyg. 99 : 128–141.
  14. Halstead SB, Scanlon JE, Umpaivit P, Udomsakdi S, 1969. Dengue and Chikungunya virus infection in man in Thailand, 1962–1964. IV. Epidemiologic studies in the Bankok metropolitan area. Am J Trop Med Hyg 18 : 997–1021.
  15. Rao TR, 1966. Recent epidemics caused by Chikungunya virus in India, 1963–1965. Sci Culture 32 : 215.
  16. Porter KR, Tan R, Istary Y, Suharyono W, Sutaryo, Widjaja S, Ma’Roef C, Listiyaningsih E, Kosasih H, Hueston L, McArdle J, Juffrie M, 2004. A serological study of Chikungunya virus transmission in Yogyakarta, Indonesia: evidence for the first outbreak since 1982. Southeast Asian J Trop Med Public Health 35 : 408–415.
  17. Pastorino B, Muyembe-Tamfum JJ, Bessaud M, Tock F, Tolou H, Durand JP, Peyrefitte CN, 2004. Epidemic resurgence of Chikungunya virus in Democratic Republic of the Congo: identification of a new central African strain. J Med Virol 74 : 277–282.
  18. Vanlandingham DL, Hong C, Klingler K, Tsetsarkin K, McElroy KL, Powers AM, Lehane MJ, Higgs S, 2005. Differential infectivities of o’nyong nyong and chikungunya virus isolates in Anopheles gambiae and Aedes aegypti mosquitoes. Am J Trop Med Hyg. 72 : 616–621.
  19. Vanlandingham DL, Tsetsarkin K, Hong C, Klingler K, McElroy KL, Lehane MJ, Higgs S, 2005. Development and characterization of a double subgenomic chikungunya virus infectious clone to express heterologous genes in Aedes aegypti mosquitoes. Insect Biochem Mol Biol 35 : 1162–1170.
  20. Brault AC, Foy BD, Myles KM, Kelly CL, Higgs S, Weaver SC, Olson KE, Miller BR, Powers AM, 2004. Infection patterns of o’nyong nyong virus in the malaria-transmitting mosquito, Anopheles gambiae. Insect Mol Biol 13 : 625–635.
  21. Higgs S, Olson KE, Kamrud KI, Powers AM, Beaty BJ, 1997. Viral expression systems and viral infections in insects. Crampton JM, Beard CB, Louis C, eds. The Molecular Biology of Disease Vectors: A Methods Manual. London: Chapman and Hall, 457–483.
  22. Woodward TM, Miller BR, Beaty BJ, Trent DW, Roehrig JT, 1991. A single amino acid change in the E2 glycoprotein of Venezuelan equine encephalitis virus affects replication and dissemination in Aedes aegypti mosquitoes. J Gen Virol 72 : 2431–2435.
  23. Miller BR, Mitchell CJ, 1991. Genetic selection of a flavivirus-refractory strain of the yellow fever mosquito Aedes aegypti. Am J Trop Med Hyg 45 : 399–407.
  24. Higgs S, Beaty BJ, 1996. Rearing and containment of arthropod vectors. Marquardt WC, Beaty BJ, eds. Biology of Disease Vectors. Niwot, CO: University Press of Colorado, 595–605.
  25. Cosgrove JB, Wood RJ, Petric D, Evans DT, Abbott RH, 1994. A convenient mosquito membrane feeding system. J Am Mosq Control Assoc 10 : 434–436.
  26. Kuhn RJ, Griffin DE, Owen KE, Niesters HG, Strauss JH, 1996. Chimeric Sindbis-Ross River viruses to study interactions between alphavirus nonstructural and structural regions. J Virol 70 : 7900–7909.
  27. Wilkinson TA, Tellinghuisen TL, Kuhn RJ, Post CB, 2005. Association of Sindbis virus capsid protein with phospholipid membranes and the E2 glycoprotein: implications for alphavirus assembly. Biochemistry 44 : 2800–2810.
  28. Lopez S, Yao JS, Kuhn RJ, Strauss EG, Strauss JH, 1994. Nucleo-capsid-glycoprotein interactions required for assembly of alphaviruses. J Virol 68 : 1316–1323.
  29. Frolov I, Frolova E, Schlesinger S, 1997. Sindbis virus replicons and Sindbis virus: assembly of chimeras and of particles deficient in virus RNA. J Virol 71 : 2819–2829.
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  • Received : 20 Sep 2005
  • Accepted : 19 Nov 2005

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