Volume 74, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


The alphaviruses o’nyong nyong virus (ONNV) and chikungunya virus (CHIKV) provide a unique system to study the viral genes involved in vector specificity. ONNV infects both anopheline and culicine mosquitoes, whereas CHIKV infects only culicine mosquitoes. In this study, chimeric viruses were constructed that contained genes from both ONNV and CHIKV. These chimeras and previously described full-length infectious clones of ONNV and CHIKV were evaluated in and mosquitoes. Virus derived from the infectious clones of ONNV and CHIKV retained the vector specificity of the parental viruses. All six of the chimeras were found to infect mosquitoes at high rates but only the chimera containing viral genes encoding all of the structural proteins of ONNV was able to infect mosquitoes. These data indicate that all of the viral structural proteins are necessary for ONNV to infect mosquitoes.


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  • Received : 20 Sep 2005
  • Accepted : 19 Nov 2005

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