1921
Volume 74, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Monitoring the spread of the knockdown resistance allele in areas of extensive pyrethroid use is critical to vector-control projects. Currently available methods for detecting from DNA samples are characterized by poor amplification, time-consuming steps, and primers that exhibit frequent null alleles. We describe a new PCR diagnostic that uses fluorescent primers based on conserved priming sites and enables simple detection of the allele on a sequencer. Using samples from a West African population, we show that the new PCR yielded significantly higher rates of amplification and more accurate estimates of frequency. The method works equally well for the leucine to phenylalanine substitution found in West Africa and the East African leucine to serine substitution.

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References

  1. World Health Organization, 1999. The World Health Report 1999: Making a difference. Geneva, World Health Organization.
  2. Marshall E, 2001. A renewed assault on an old and deadly foe. Science 290 : 428–430. [Google Scholar]
  3. Coluzzi M, Sabatini A, 1967. Cytogenetic observations on species A and B of the Anopheles gambiae complex. Parassitologia 9 : 71–88. [Google Scholar]
  4. Davidson G, Paterson HE, Coluzzi M, Mason GF, Micks DW, 1967. The Anopheles gambiae complex. Wright JW, Pal R, eds. Genetics of Insect Vectors of Disease. Amsterdam: Elsevier, 211–250.
  5. Coetzee M, Craig M, Le Sueur D, 2000. Distribution of African malaria mosquitoes belonging to the Anopheles gambiae complex. Parasitol Today 16 : 74–77. [Google Scholar]
  6. Bryan JH, Di Deco MA, Petrarca V, Coluzzi M, 1982. Inversion polymorphism and incipient speciation in Anopheles gambiae s. str. in the Gambiae, West Africa. Genetica 59 : 167–176. [Google Scholar]
  7. Touré YT, Petrarca V, Coluzzi M, 1983. Nueva entita del complesso Anopheles gambiae in Mali. Parrasitologia 25 : 367–370. [Google Scholar]
  8. Coluzzi M, Petrarca V, Di Deco MA, 1985. Chromosomal inversion intergradation and incipient speciation in Anopheles gambiae. Bollettino di Zoologia 52 : 45–63. [Google Scholar]
  9. Favia G, Della Torre A, Bagyaoko M, Lanfrancotti A, Sagnon NF, Toure YT, Coluzzi M, 1997. Molecular identifications of sympatric chromosomal forms of Anopheles gambiae and further evidence of their reproductive isolation. Insect Mol Biol 6 : 377–383. [Google Scholar]
  10. Favia G, Lanfrancotti A, Spanos L, Sidén-Kiamos I, Louis C, 2001. Molecular characterization of ribosomal DNA (rDNA) polymorphisms discriminating among chromosomal forms of Anopheles gambiae s.s. Insect Mol Biol 10 : 19–23. [Google Scholar]
  11. Della Torre A, Costantini C, Besansky NJ, Caccone A, Petrarca V, Powell JR, Coluzzi M, 2002. Speciation within Anopheles gambiae - the glass is half full. Science 298 : 115–117. [Google Scholar]
  12. Gentile G, Della Torre A, Maegga B, Powell JR, Caccone A, 2002. Genetic differentiation in the African malaria vector, Anopheles gambiae s.s., and the problem of taxonomic status. Genetics 161 : 1561–1578. [Google Scholar]
  13. Wondji C, Simard F, Fontenille D, 2002. Evidence for genetic differentiation between the molecular forms M and S within the Forest chromosomal form of Anopheles gambiae in an area of sympatry. Insect Mol Biol 11 : 11–19. [Google Scholar]
  14. Choi HW, Breman JG, Teutsch SM, Liu S, Hightower AW, Sexton JD, 1995. The effectiveness of insecticide-impregnated bed nets in reducing cases of malaria infection: a meta-analysis of published results. Am J Trop Med Hyg 52 : 377–382. [Google Scholar]
  15. Mittal PK, Adak T, Sharma VP, 1991. Acute toxicity of certain organochlorine, organophosphorus, synthetic pyrethroid and microbial insecticides to the mosquito fish Gambusia affinis (Baird and Girard). Indian J Malariol 28 : 167–170. [Google Scholar]
  16. Hemingway J, Ranson H, 2000. Insecticide resistance in insect vectors of human disease. Annu Rev Entomol 45 : 371–391. [Google Scholar]
  17. Ranson H, Jensen B, Vulule JM, Wang X, Hemingway J, Collins FH, 2000. Identification of a point mutation in the voltage-gated sodium channel gene of Kenyan Anopheles gambiae associated with resistance to DDT and pyrethroids. Insect Mol Biol 9 : 491–497. [Google Scholar]
  18. Martinez-Torres D, Chandre F, Williamson MS, Darriet F, Bergé JB, Devonshire AL, Guillet P, Pasteur N, Pauron D, 1998. Molecular characterization of pyrethroid knockdown resistance (kdr) in the major malaria vector Anopheles gambiae s.s. Insect Mol Biol 7 : 179–184. [Google Scholar]
  19. Elissa N, Mouchet J, Riviere F, Meunier JY, Yao K, 1993. Resistance of Anopheles gambiae s.s. to pyrethroids in Cote d’Ivoire. Ann Soc Belg Med Trop 73 : 291–294. [Google Scholar]
  20. Weill M, Chandre F, Brengues C, Manguin S, Akogbeto M, Pasteur N, Guillet P, Raymond M, 2000. The kdr mutation occurs in the Mopti form of Anopheles gambiae s.s. through introgression. Insect Mol Biol 9 : 451–455. [Google Scholar]
  21. Diabate A, Baldet T, Chandre C, Dabire KR, Kengne P, Guiguemde TR, Simard F, Guillet P, Hemingway J, Hougard JM, 2003. Kdr mutation, a genetic marker to assess events of introgression between the molecular M and S forms of Anopheles gambiae (Diptera: Culicidae) in the tropical savannah area of West Africa. J Med Entomol 40 : 195–198. [Google Scholar]
  22. Diabate A, Brengues C, Baldet T, Dabire KR, Hougard JM, Akogbeto M, Kengne P, Simard F, Guillet P, Hemingway J, Chandre F, 2004. The spread of the Leu-Phe kdr mutation through Anopheles gambiae complex in Burkina Faso: genetic introgression and de novo phenomena. Trop Med Int Health 9 : 1267–1273. [Google Scholar]
  23. Gentile G, Santolamazza F, Fanello C, Petrarca V, Caccone A, della Torre A, 2004. Variation in an intron sequence of the voltage-gated sodium channel gene correlates with genetic differentiation between Anopheles gambiae s.s. molecular forms. Insect Mol Biol 13 : 371–377. [Google Scholar]
  24. Fanello C, Petrarca V, della Torre A, Santolamazza F, Dolo G, Coulibaly M, Alloueche A, Curtis CF, Touré YT, Coluzzi M, 2003. The pyrethroid knock-down resistance gene in the Anopheles gambiae complex in Mali and further indication of incipient speciation within An. gambiae s.s. Insect Mol Biol 12 : 241–245. [Google Scholar]
  25. Yawson AE, McCall PJ, Wilson MD, Donnely MJ, 2004. Species abundance and insecticide resistance of Anopheles gambiae in selected areas of Ghana and Burkina Faso. Med. Vet. Entomol 18 : 372–377. [Google Scholar]
  26. Stump AD, Atieli FK, Vulule JM, Besansky NJ, 2004. Dynamics of the pyrethroid knockdown resistance allele in western Kenyan populations of Anopheles gambiae in response to insecticide-treated bed net trials. Am J Trop Med Hyg 70 : 591–596. [Google Scholar]
  27. Lanzaro GC, Tripet F, 2003. Gene flow among populations of Anopheles gambiae: a critical review. Takken W, Scott TW, eds. Ecological Aspects for the Application of Genetically Modified Mosquitoes. Wageningen, The Netherlands: Frontis Press, 109–132.
  28. Reimer LJ, Tripet F, Slotman M, Spielman A, Fondjo E, Lanzaro GC, 2005. An unusual distribution of the kdr gene among populations of Anopheles gambiae on the island of Bioko, Equatorial Guinea. Insect Mol Biol 14 : 683–688. [Google Scholar]
  29. Kolaczinski JH, Fanello C, Hervé J-P, Conway DJ, Carneval P, Curtis F, 2000. Experimental and molecular genetic analysis of the impact of pyrethroid and non-pyrethroid insecticide impregnated bednets for mosquito control in an area of pyrethroid resistance. Bull Entomol Res 9 : 125–132. [Google Scholar]
  30. Lynd A, Ranson H, McCall PJ, Randle NP, Black WC IV, Walker ED, Donelly MJ, 2005. A simplified high-throughput method for pyrethroid knock-down resistance (kdr) detection in Anopheles gambiae. Malaria J 4 : 16. [Google Scholar]
  31. Scott JA, Brogdon WG, Collins FH, 1993. Identification of single specimens of the Anopheles gambiae complex by the polymerase chain reaction. Am J Trop Med Hyg 49 : 520–529. [Google Scholar]
  32. Fanello C, Santolamazza F, Della Torre A, 2002. Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP. Med Vet Entomol 16 : 461–464. [Google Scholar]
  33. Rychlik W, 2002. Oligo—Primer Analysis software. Version 6. Molecular Insights, West Cascade, CO.
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  • Received : 18 Oct 2005
  • Accepted : 08 Nov 2005

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