1921
Volume 74, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Improving strategies for diagnosing infection by the four human species parasites is important as field-based epidemiologic and clinical studies focused on malaria become more ambitious. Expectations for malaria diagnostic assays include rapid processing with minimal expertise, very high specificity and sensitivity, and quantitative evaluation of parasitemia to be delivered at a very low cost. Toward fulfilling many of these expectations, we have developed a post–polymerase chain reaction (PCR)/ligase detection reaction–fluorescent microsphere assay (LDR-FMA). This assay, which uses Luminex FlexMAP™ microspheres, provides simultaneous, semi-quantitative detection of infection by all four human malaria parasite species at a sensitivity and specificity equal to other PCR-based assays. In blinded studies using -infected blood from cultures, we identified infected and uninfected samples with 100% concordance. Additionally, in analyses of cultures and -infected monkeys, comparisons between parasitemia and LDR-FMA signal intensity showed very strong positive correlations (r > 0.95). Application of this multiplex species LDR-FMA diagnostic assay will increase the speed, accuracy, and reliability of diagnosing human species infections in epidemiologic studies of complex malaria-endemic settings.

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2006-03-01
2017-09-22
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  • Received : 13 Jul 2005
  • Accepted : 29 Aug 2005

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